Indlela yokwahlulahlula imixube ngaphakathi kwe-centrifuge
Intshayelelo kwiCentrifugation
I-Centrifugation yindlela esetyenziswa kakhulu kwiindawo ezahlukeneyo zesayensi, zonyango, kunye nezoshishino ukwahlula imixube ngokusekelwe kubuninzi bayo. Le nkqubo isebenzayo ibandakanya ukusetyenziswa komatshini we-centrifuge, osebenzisa amandla e-centrifugal ukwahlula izinto zomxube. Kwiminyaka edlulileyo, i-centrifugation ibe sisixhobo esibalulekileyo kwiilabhoratri zophando, amaziko okuxilonga, kunye neendawo zoshishino ngenxa yokukwazi kwayo ukukhupha izinto ezithile kunye namasuntswana kwimixube enzima. Kweli nqaku, siza kujonga kwihlabathi elinomdla le-centrifugation kwaye sihlolisise indlela imixube eyahlulwa ngayo ngaphakathi kwe-centrifuge.
Ukuqonda Amandla E-Centrifugal
Ukuze uqonde indlela imixube eyahlulwa ngayo ngaphakathi kwi-centrifuge, kubalulekile ukuqonda amandla e-centrifugal. Xa into ifakwa kwi-centrifuge, ijikeleza ngokukhawuleza, idala amandla e-centrifugal ajoliswe ngaphandle ukusuka embindini. Izinto ezikumxube zifumana ubukhulu obahlukeneyo balo mandla ngokusekelwe kubuninzi bazo. Amasuntswana anzima anyanzelwa ukuya kwimiphetho engaphandle yegumbi elijikelezayo, elibizwa ngokuba yintsimi yamandla e-centrifugal.
Ukwahlulwa kwenkunkuma: Ukwahlulwa ngobuninzi
Enye yeendlela eziphambili ezisetyenziswa kwi-centrifuge kukusasazwa kwe-sedimentation. Kule nkqubo, izinto ezixineneyo ngaphakathi komxube zihlala ezantsi kwetyhubhu ye-centrifuge, zenze i-pellet okanye i-sediment. Oku kuvumela ukwahlukana kwezinto ezinobunzima obahlukeneyo. Umzekelo, ekuhlukaniseni igazi, iiseli ezibomvu zegazi (ii-RBC) zixineneyo kwaye zihlala ezantsi, ngelixa i-plasma, equlethe ezinye izinto, ihlala kwi-supernatant. Ngokususa i-supernatant ngononophelo, ii-RBC zinokuhlukaniswa ukuze kuhlalutywe ngakumbi okanye kusetyenziswe iinkqubo zonyango.
Isantya seCentrifugation kunye noLungiso lweXesha
Impumelelo ye-centrifugation ixhomekeke kakhulu ekumiseleni isantya esifanelekileyo kunye nobude bokwahlukana. Izinto ezahlukeneyo okanye amasuntswana anokufuna useto oluhlukeneyo lwe-centrifuge ukuze kufezekiswe ukwahlukana okufanelekileyo. Ngokulungisa isantya kunye nexesha lokujikeleza, izazinzulu zinokwenza ngokwezifiso inkqubo ukuze zijolise ngokukodwa kwizinto okanye amasuntswana afunekayo. Isantya esikhawulezayo senza amandla aphezulu e-centrifugal, enceda ekwahlulweni kwamasuntswana amancinci okanye iikhompawundi. Nangona kunjalo, amandla amaninzi e-centrifugal anokonakalisa amasuntswana okanye iiseli ezibuthathaka, nto leyo egxininisa imfuneko yokufumana ibhalansi efanelekileyo.
Iindlela zokufaka i-Centrifugation: i-Differential kunye ne-Density Gradient
Ngaphakathi kwe-centrifugation, iindlela ezimbini eziphambili zisetyenziswa ukwahlula imixube: i-differential centrifugation kunye ne-density gradient centrifugation. I-differential centrifugation ibandakanya imijikelo emininzi ye-centrifugation ngesantya esonyukayo, okuvumela ukwahlulwahlulwa kwezixhobo ezahlukeneyo kwizigaba ezahlukeneyo. Ngokulungisa ubude kunye nesantya senyathelo ngalinye elilandelayo, izazinzulu zinokuhlulahlula kancinci kancinci izixhobo ezinobukhulu kunye noxinano olwahlukeneyo.
Kwelinye icala, i-centrifugation ye-density gradient isebenzisa ityhubhu ye-centrifuge equlathe isisombululo se-density gradient. Esi sisombululo senza i-concentration gradient, evumela amasuntswana ukuba ahlale kwiindawo ezahlukeneyo ngokusekelwe kubuninzi bawo. Ngokubeka umxube ngononophelo phezu kwe-gradient, amasuntswana anobunzima obufanayo aya kwiindawo ezithile ngaphakathi kwityhubhu ye-centrifuge, okwenza kube lula ukwahlukana kwawo. I-density gradient centrifugation iluncedo ngakumbi xa kujongwana nemixube equlethe izinto ezinxulumeneyo okanye amasuntswana ahluke kancinci kubunzima.
Isiphelo:
I-Centrifugation yindlela eguquguqukayo nebalulekileyo yokwahlula imixube kwiindlela ezininzi zesayensi, zonyango, kunye nezoshishino. Ngokusebenzisa amandla e-centrifugal kunye ne-sedimentation, izazinzulu zinokufikelela ekuhlukaniseni ngokuchanekileyo izinto ngokusekelwe kubuninzi bazo. Ngokusebenzisa kakuhle useto lwe-centrifuge kunye nokusebenzisa iindlela ezifana ne-differential kunye ne-density gradient centrifugation, abaphandi banokuhlukanisa izinto ezithile okanye amasuntswana ngokuchanekileyo nangokusebenza kakuhle. Ngokuqhubeka nokuqhubela phambili kwitekhnoloji ye-centrifuge, esi sixhobo sibalulekileyo siyaqhubeka nokuguqula iinkalo ezahlukeneyo zokufunda kunye nokuphucula ukuqonda kwethu imixube enzima.
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