yuav ua li cas cov khoom sib xyaw sib cais hauv lub centrifuge
Kev Taw Qhia Txog Centrifugation
Kev siv centrifugation yog ib txoj kev siv dav hauv ntau yam kev tshawb fawb, kev kho mob, thiab kev lag luam rau kev sib cais cov khoom sib xyaw raws li lawv qhov ceev. Cov txheej txheem zoo no suav nrog kev siv lub tshuab centrifuge, uas siv lub zog centrifugal los cais cov khoom sib xyaw. Tau ntau xyoo, centrifugation tau dhau los ua ib qho cuab yeej tseem ceeb hauv cov chaw soj ntsuam, cov chaw kuaj mob, thiab cov chaw lag luam vim nws muaj peev xwm rho tawm cov tshuaj tshwj xeeb thiab cov khoom me me los ntawm cov khoom sib xyaw nyuaj. Hauv tsab xov xwm no, peb yuav nkag mus rau hauv lub ntiaj teb zoo kawg nkaus ntawm centrifugation thiab tshawb nrhiav seb cov khoom sib xyaw raug sib cais li cas hauv lub centrifuge.
Nkag Siab Txog Lub Zog Centrifugal
Yuav kom nkag siab txog kev sib cais cov khoom sib xyaw hauv lub centrifuge, nws yog ib qho tseem ceeb kom nkag siab txog lub zog centrifugal. Thaum muab ib yam khoom tso rau hauv lub centrifuge, nws tig sai sai, tsim lub zog centrifugal uas taw qhia tawm ntawm qhov chaw nruab nrab. Cov khoom hauv cov khoom sib xyaw muaj qhov sib txawv ntawm lub zog no raws li lawv qhov ceev. Cov khoom hnyav dua raug yuam mus rau ntug sab nraud ntawm lub chamber tig, hu ua lub zog centrifugal.
Kev Tso Tawm: Kev Sib Cais Los Ntawm Qhov Ceev
Ib qho ntawm cov txheej txheem tseem ceeb uas siv hauv lub centrifuge yog kev tso dej tawm. Hauv cov txheej txheem no, cov khoom sib xyaw ua ke ntau dua hauv cov khoom sib xyaw yuav poob rau hauv qab ntawm lub raj centrifuge, tsim cov pellet lossis cov av. Qhov no tso cai rau kev sib cais ntawm cov khoom sib xyaw uas muaj qhov sib txawv. Piv txwv li, hauv kev sib cais ntshav, cov qe ntshav liab (RBCs) yog qhov ntom dua thiab poob rau hauv qab, thaum cov plasma, uas muaj lwm cov khoom, tseem nyob hauv cov supernatant. Los ntawm kev ua tib zoo tshem tawm cov supernatant, cov RBCs tuaj yeem raug cais tawm rau kev tshuaj xyuas ntxiv lossis cov txheej txheem kho mob.
Kev Ceev thiab Lub Sijhawm Centrifugation Zoo Tshaj Plaws
Qhov kev vam meej ntawm centrifugation feem ntau nyob ntawm kev txiav txim siab qhov ceev thiab lub sijhawm tsim nyog rau kev sib cais. Cov khoom sib txawv lossis cov khoom me me yuav xav tau ntau qhov chaw centrifuge kom ua tiav kev sib cais zoo tshaj plaws. Los ntawm kev kho qhov ceev thiab lub sijhawm tig, cov kws tshawb fawb tuaj yeem kho cov txheej txheem kom tsom mus rau cov khoom lossis cov khoom me me uas xav tau. Qhov ceev ceev dua tsim cov zog centrifugal siab dua, pab txhawb kev sib cais ntawm cov khoom me me lossis cov khoom sib xyaw. Txawm li cas los xij, cov zog centrifugal ntau dhau tuaj yeem ua rau cov khoom me me lossis cov cell puas tsuaj, qhia txog qhov tsim nyog ntawm kev nrhiav qhov sib npaug zoo.
Cov Txuj Ci Centrifugation: Kev Sib Txawv thiab Kev Nrawm Gradient
Hauv thaj chaw ntawm centrifugation, ob txoj kev tseem ceeb yog siv rau kev sib cais cov khoom sib xyaw: differential centrifugation thiab density gradient centrifugation. Differential centrifugation cuam tshuam ntau qhov kev sib hloov ntawm centrifugation ntawm qhov ceev nce ntxiv, tso cai rau kev sib cais ntawm cov khoom sib txawv ntawm ntau theem. Los ntawm kev kho lub sijhawm thiab qhov ceev rau txhua kauj ruam tom ntej, cov kws tshawb fawb tuaj yeem maj mam sib cais cov khoom nrog ntau qhov ntau thiab tsawg thiab qhov ceev.
Ntawm qhov tod tes, kev centrifugation density gradient siv lub raj centrifuge uas muaj cov kua density gradient. Cov kua no tsim kom muaj qhov concentration gradient, uas cia cov khoom me me nyob rau ntau qhov chaw sib txawv raws li lawv qhov densities. Los ntawm kev ua tib zoo txheej cov khoom sib xyaw rau saum qhov gradient, cov khoom me me uas muaj qhov density zoo sib xws yuav tsiv mus rau qhov chaw tshwj xeeb hauv lub raj centrifuge, ua kom lawv sib cais tau yooj yim. Kev centrifugation density gradient yog qhov tshwj xeeb tshaj yog thaum cuam tshuam nrog cov khoom sib xyaw uas muaj cov khoom sib txuas ze lossis cov khoom me me uas tsuas yog txawv me ntsis hauv qhov density.
Xaus lus:
Kev siv centrifugation yog ib txoj kev siv tau ntau yam thiab tseem ceeb rau kev sib cais cov khoom sib xyaw hauv ntau yam kev tshawb fawb, kev kho mob, thiab kev lag luam. Los ntawm kev siv lub zog centrifugal thiab kev tso dej tawm, cov kws tshawb fawb tuaj yeem ua tiav kev sib cais ntawm cov khoom raws li lawv qhov ceev. Los ntawm kev ua kom zoo dua qhov chaw centrifuge thiab siv cov txheej txheem xws li kev sib txawv thiab qhov ceev gradient centrifugation, cov kws tshawb fawb tuaj yeem cais cov khoom tshwj xeeb lossis cov khoom me me nrog qhov tseeb thiab ua haujlwm tau zoo. Nrog rau kev nce qib hauv kev siv tshuab centrifuge, cov cuab yeej tseem ceeb no txuas ntxiv hloov pauv ntau yam kev kawm thiab txhawb peb txoj kev nkag siab txog cov khoom sib xyaw ua ke.
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