Misanganiswa inoparadzaniswa sei mukati me centrifuge

Nhanganyaya yeCentrifugation

Kupinza zvinhu mu "centrifugation" inzira inoshandiswa zvakanyanya muzvikamu zvakasiyana-siyana zvesainzi, zvekurapa, uye zvemaindasitiri pakuparadzanisa misanganiswa zvichienderana nekuwanda kwayo. Maitiro aya anoshanda anosanganisira kushandisa muchina we "centrifuge", uyo unoshandisa simba re "centrifugal" kuparadzanisa zvikamu zvemusanganiswa. Mumakore apfuura, "centrifugation" yave chishandiso chakakosha mumarabhoritari ekutsvagisa, nzvimbo dzekuongorora, uye nzvimbo dzemaindasitiri nekuda kwekugona kwayo kubvisa zvinhu zvakati wandei nezvidimbu kubva mumisanganiswa yakaoma. Muchinyorwa chino, tichaongorora nyika inonakidza ye "centrifugation" uye kuongorora kuti misanganiswa inoparadzaniswa sei mukati me "centrifuge".

Kunzwisisa Simba reCentrifugal

Kuti unzwisise kuti misanganiswa inoparadzaniswa sei mukati me centrifuge, zvakakosha kunzwisisa simba re centrifugal. Kana chinhu chikaiswa mu centrifuge, chinotenderera nekukurumidza, zvichigadzira simba re centrifugal rinotungamirwa kunze kubva pakati. Zvikamu zviri mumusanganiswa zvinosangana nehukuru hwakasiyana hwesimba iri zvichienderana nekuwanda kwazvo. Zvidimbu zvinorema zvinomanikidzwa kuenda kumucheto wekunze wekamuri rinotenderera, rinonzi centrifugal force field.

Kucherwa kwevhu: Kuparadzaniswa neKuwanda kwevhu

Imwe yenzira huru dzinoshandiswa mu centrifuge ndeye sedimentation. Mukuita uku, zvikamu zvakakora mukati memusanganiswa zvinogara pasi pechubhu ye centrifuge, zvichiita pellet kana sediment. Izvi zvinobvumira kupatsanurwa kwezvikamu zvine densities dzakasiyana. Semuenzaniso, mukupatsanurwa kweropa, masero matsvuku eropa (RBCs) anokora uye anogara pasi, nepo plasma, ine zvimwe zvikamu, ichiramba iri mu supernatant. Nekubvisa supernatant nokungwarira, ma RBC anogona kuparadzaniswa kuti aongororwe zvakanyanya kana kuti kurapwa.

Kukurumidza kweCentrifugation uye Kugadzirisa Nguva

Kubudirira kwe centrifugation kunonyanya kutsamira pakuona kumhanya kwakakodzera uye nguva yekuparadzanisa. Zvinhu zvakasiyana-siyana kana zvidimbu zvinogona kuda magadzirirwo akasiyana e centrifuge kuti pave nekupatsanurwa kwakakodzera. Nekugadzirisa kumhanya kwenguva nenguva, masayendisiti anogona kugadzirisa maitiro acho kuti anyatsonanga zvikamu kana zvidimbu zvinodiwa. Kumhanya nekukurumidza kunogadzira masimba e centrifugal akakwira, zvichibatsira mukupatsanurwa kwezvidimbu kana makemikari akapfava. Zvisinei, masimba e centrifugal akawandisa anogona kukuvadza zvidimbu kana masero asina kusimba, zvichisimbisa kukosha kwekuwana chiyero chakakodzera.

Maitiro eCentrifugation: Kusiyana uye Density Gradient

Mukati menyaya ye centrifugation, nzira mbiri huru dzinoshandiswa pakupatsanura misanganiswa: differential centrifugation uye density gradient centrifugation. Differential centrifugation inosanganisira madenderedzwa akawanda e centrifugation nekumhanya kunowedzera, zvichibvumira kupatsanurwa kwezvikamu zvakasiyana pamatanho akasiyana. Nekugadzirisa nguva nekumhanya kwedanho rega rega rinotevera, masayendisiti anogona kupatsanura zvishoma nezvishoma zvikamu zvine saizi dzakasiyana uye densities.

Kune rumwe rutivi, density gradient centrifugation inoshandisa chubhu yecentrifuge ine density gradient solution. Iyi solution inogadzira concentration gradient, zvichibvumira zvidimbu kuti zvigare panzvimbo dzakasiyana zvichienderana ne density yazvo. Nekuisa musanganiswa wacho pamusoro pe gradient nokungwarira, zvidimbu zvine density dzakafanana zvinotamira kunzvimbo dzakatarwa mukati mechubhu yecentrifuge, zvichiita kuti zviparadzane. Density gradient centrifugation inonyanya kubatsira pakubata nemisanganiswa ine zvikamu zvakabatana kana zvidimbu zvinosiyana zvishoma mu density.

Mhedziso:

Kugadzira centrifugation inzira inoshandiswa zvakasiyana-siyana uye inokosha yekuparadzanisa misanganiswa mune zvakawanda zvesainzi, zvokurapa, uye maindasitiri. Kuburikidza nekushandisa simba re centrifugal uye sedimentation, masayendisiti anogona kuwana kupatsanurwa kwakanyatsojeka kwezvikamu zvichienderana nehuwandu hwazvo. Nekugadzirisa marongero e centrifuge uye kushandisa matekiniki akadai se differential uye density gradient centrifugation, vaongorori vanogona kusiyanisa zvinhu kana zvidimbu zvakananga uye nemazvo. Nekufambira mberi kuri kuitika mu centrifuge technology, chishandiso ichi chakakosha chinoramba chichichinja nzvimbo dzakasiyana dzekudzidza uye kuwedzera kunzwisisa kwedu misanganiswa yakaoma.