Pehea e hoʻokaʻawale ai ka centrifugation i nā hui ʻana

Centrifugation: He ʻenehana ikaika no ka hoʻokaʻawale ʻana i nā hui

Hoʻolauna:

He ʻenehana hoʻokolohua i hoʻohana nui ʻia ka centrifugation no ka hoʻokaʻawale ʻana i nā hui ʻana ma muli o ko lākou mau helu sedimentation i loko o kahi kahua ikaika centrifugal. ʻAe kēia ʻenehana i ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe ʻana i nā ʻāpana like ʻole i loko o kahi hui ʻana, me nā mea paʻa, nā wai, a me nā ʻāpana i hoʻokuʻu ʻia i loko o kahi hoʻokuʻu.

1. Nā Kumumanaʻo Kumu o ka Centrifugation:

I mea e maopopo ai pehea e hana ai ka centrifugation, he mea nui e loaʻa ka ʻike i kona mau loina kumu. Ma kona kumu, hilinaʻi ka centrifugation i ke kumumanaʻo o ka sedimentation, ʻo ia hoʻi ka hoʻonoho ʻana o nā ʻāpana ʻoi aku ka paʻa ma lalo o ka mana o ka umekaumaha. Ma ka hoʻopili ʻana i ka ikaika centrifugal, ʻoi aku ka ikaika ma mua o ka ikaika umekaumaha, hiki ke hoʻokaʻawale pono ʻia nā mea me nā densities like ʻole.

2. Ke hoʻomākaukau ʻana i ka hāpana no ka Centrifugation:

Ma mua o ka hana ʻana i ka centrifugation, he mea nui e hoʻomākaukau pono i ka hāpana. Ma muli o ke ʻano o ka hui ʻana e kālailai ʻia ana, pono paha nā ʻanuʻu pre-centrifugation like ʻole. Hiki i kēia ke pili i ka hoʻohui ʻana i kahi hopena buffer kikoʻī, ka centrifugation medium, a i ʻole ka mālama mua ʻana i ka hāpana e wehe i nā mea makemake ʻole e hoʻopilikia i ke kaʻina hana hoʻokaʻawale.

3. Nā ʻAno Centrifugation a me kā lākou mau noi:

Hiki ke hoʻokaʻawale ʻia ka centrifugation i nā ʻano like ʻole e pili ana i nā mea e like me ka wikiwiki, ke ʻano hana, a me ka hoʻohana i manaʻo ʻia. ʻO nā ʻano maʻamau o nā ʻano hana hoʻokaʻawale centrifugal i hoʻohana ʻia i loko o nā keʻena hoʻokolohua e komo pū ana me ka centrifugation differential, density gradient centrifugation, a me ka ultracentrifugation. E nānā pono kākou i kēia mau ʻano a me kā lākou mau hoʻohana kikoʻī.

3.1 Centrifugation ʻokoʻa:

ʻO ka centrifugation ʻokoʻa, i ʻike ʻia hoʻi ʻo ka centrifugation fractionation, he ʻano hana i hoʻohana nui ʻia e hoʻokaʻawale i nā ʻāpana o nā nui like ʻole. Hana kēia ʻano hana ma ka hoʻonui mālie ʻana i ka ikaika centrifugal e hoʻomoe a hōʻiliʻili i nā ʻāpana a i ʻole nā ​​​​ʻāpana e pili ana i ko lākou nui a me ka nui. ʻO kahi laʻana o kāna noi ʻana, ʻo ia ka hoʻokaʻawale ʻana i nā organelles cellular mai nā cell homogenized.

3.2 Ke Keʻena Hoʻokaʻawale ʻana o ka Gradient Density:

ʻO ka centrifugation gradient density e pili ana i ke kau ʻana i kahi hāpana ma luna o kahi medium gradient density, e like me ka sucrose a i ʻole cesium chloride. I ka milo ʻana o ka centrifuge, neʻe nā ʻāpana i loko o ka hāpana ma o ka medium gradient density a hiki i ko lākou kūlana kaulike ma muli o ko lākou density. He mea pono loa kēia ʻenehana no ka hoʻokaʻawale ʻana i nā ʻāpana a i ʻole nā ​​​​ʻāpana i loaʻa ka nui like akā nā densities like ʻole. Loaʻa iā ia nā noi i ka hoʻomaʻemaʻe ʻana i nā virus, nā protein, a me nā waikawa nucleic.

3.3 Hoʻokaʻawale ʻana i nā mea he nui:

He ʻenehana ikaika ka Ultracentrifugation i hoʻohana ʻia e hoʻokaʻawale i nā ʻāpana ma muli o ko lākou ʻano, ka nui, a me ka nui. Ma ka hoʻohana ʻana i nā wikiwiki kiʻekiʻe a me nā manawa holo lōʻihi, hiki i ka ultracentrifugation ke hoʻokaʻawale pono ma waena o nā ʻāpana i ʻokoʻa i kekahi mau nanometer a i ʻole milligrams. Hoʻohana pinepine ʻia kēia ʻenehana i ka biochemistry a me ka biology molecular no ka hoʻokaʻawale ʻana i nā ʻāpana subcellular, ke aʻo ʻana i nā pilina protein-protein, a me ka hoʻoholo ʻana i ke kaumaha molecular o nā macromolecules.

4. Nā kumu e pili ana i ka pono o ka Centrifugation:

Hiki i kekahi mau kumu ke hoʻohuli i ka pono a me ka holomua o ke kaʻina hana centrifugation. ʻO kēia mau mea ka wikiwiki o ka centrifuge, ka lōʻihi o ka holo ʻana, ka mahana, a me ke koho ʻana o nā paipu centrifuge a i ʻole nā ​​rotors. ʻO ka hoʻomaopopo ʻana i kēia mau mea a me ka hoʻomaikaʻi ʻana iā lākou hiki ke hoʻomaikaʻi nui i nā hopena hoʻokaʻawale i loaʻa mai ka centrifugation.

5. Nā Pōmaikaʻi a me nā Palena o ka Centrifugation:

Hāʻawi ka Centrifugation i kekahi mau pono ma luna o nā ʻano hana hoʻokaʻawale ʻē aʻe. He ʻano wikiwiki a maʻalahi hoʻi ia e hiki ke lawelawe i kahi ākea o nā nui o ka laʻana a he kiʻekiʻe ka helu hana hou ʻana. Hiki ke hoʻonui maʻalahi ʻia ke ʻano hana no nā noi ʻoihana. Eia nō naʻe, he mau palena ko ka centrifugation, e like me ka hōʻino ʻana o ka laʻana ma nā wikiwiki kiʻekiʻe, ka pono no nā lako kūikawā, a me nā palena i ka hoʻokaʻawale ʻana i nā ʻāpana me nā densities like.

Hopena:

He ʻano hana koʻikoʻi ka centrifugation no ka hoʻokaʻawale ʻana i nā hui ʻana ma nā ʻano ʻepekema like ʻole a me nā ʻoihana. ʻO kona hiki ke hoʻokaʻawale a hoʻomaʻemaʻe i nā mea ma muli o ko lākou mau mānoanoa ua hoʻololi i ka noiʻi a me ka hoʻomohala ʻana ma nā ʻano aʻo like ʻole. Me ka holomua mau a me ka hoʻomohala ʻana i nā ʻano hana centrifugation hou, e hoʻomau kēia ʻano hana ikaika i ka hana koʻikoʻi i nā ʻike ʻepekema a me nā holomua ʻenehana no nā makahiki e hiki mai ana.