Centrifugation inoparadzanisa sei musanganiswa

Kuparadzanisa Misanganiswa: Nzira Ine Simba Yekuparadzanisa Misanganiswa

Nhanganyaya:

Kugadzira simbi (centrifugation) inzira inoshandiswa zvakanyanya murabhoritari yekuparadzanisa misanganiswa zvichienderana nehuwandu hwayo hwekudonha kwemvura munzvimbo ine simba repakati. Iyi nzira inobvumira kupatsanurwa nekucheneswa kwezvikamu zvakasiyana-siyana mumusanganiswa, zvinosanganisira zvinhu zvakasimba, mvura, uye kunyange zvidimbu zvakasungirirwa musimbi.

1. Nheyo Dzekutanga dzeCentrifugation:

Kuti unzwisise mashandiro anoita centrifugation, zvakakosha kuti unzwisise misimboti yayo yekutanga. Pachinyanya chayo, centrifugation inotsamira pamisimboti yekudonha kwevhu, iyo inosanganisira kugadzika kwezvikamu zvakakora pasi pesimba regiravhiti. Nekushandisa simba recentrifugal, iro rakasimba kanoverengeka kupfuura simba regiravhiti, zvinhu zvine huwandu hwakasiyana zvinogona kupatsanurwa zvinobudirira.

2. Kugadzirira Muenzaniso weCentrifugation:

Usati watanga centrifugation, zvakakosha kugadzirira sampuro nemazvo. Zvichienderana nemhando yemusanganiswa uri kuongororwa, matanho akasiyana epre-centrifugation anogona kudiwa. Izvi zvinogona kusanganisira kuwedzera imwe buffer solution, centrifugation medium, kana kutotanga kurapa sampuro kubvisa zvinhu zvisingadiwe zvinogona kukanganisa maitiro ekuparadzanisa.

3. Mhando dzeCentrifugation uye Mashandisirwo Adzo:

Kupinza simbi mu "centrifugation" kunogona kuiswa mumhando dzakasiyana-siyana zvichienderana nezvinhu zvakaita sekumhanya, nzira, uye chinangwa chekushandisa. Mhando dzinonyanya kushandiswa dze "centrifugal separation techniques" mumarabhoritari dzinosanganisira "differential centrifugation", "density gradient centrifugation", uye "ultracentrifugation". Ngatinyatsoongororai mhando idzi nemashandisirwo adzo chaiwo.

3.1 Kusiyanisa Kudzora Kupenya:

Kuparadzanisa masero (differential centrifugation), kunozivikanwawo se fractionation centrifugation, inzira inoshandiswa zvakanyanya kupatsanura zvikamu zvehukuru hwakasiyana. Nzira iyi inoshanda nekuwedzera simba re centrifugal zvishoma nezvishoma kuti riite sequentially sediment uye kuunganidza zvidimbu kana zvikamu zvichienderana nehukuru hwazvo uye density. Muenzaniso wekushandiswa kwayo kuparadzanisa masero emasero kubva kumasero akafanana.

3.2 Kudzora Kureba Kwemagiraidhi Ekuwedzera Huremu:

Kubatanidza masero emhando ye "density gradient centrifugation" kunosanganisira kuisa sample pamusoro pe "density gradient medium", senge sucrose kana cesium chloride. Sezvo "centrifuge" ichitenderera, zvidimbu zviri mukati mesample zvinofamba nepakati pe "density gradient medium" kusvika zvasvika panzvimbo yazvo ye "equilibrium" zvichienderana ne "density" yazvo. Iyi nzira inonyanya kubatsira pakuparadzanisa zvidimbu kana zvikamu zvine saizi yakafanana asi "densities" dzakasiyana. Inowana mashandisirwo mukuchenesa mavhairasi, mapuroteni, uye nucleic acids.

3.3 Kudzora ma "Ultracentrifugation":

Kupinza masero zvakanyanya inzira ine simba inoshandiswa kupatsanura zvidimbu zvichienderana nechimiro chazvo, saizi, uye huwandu hwazvo. Nekushandisa kumhanya kwakanyanya uye nguva refu, kupinza masero zvakanyanya kunogona kusiyanisa zvinobudirira zvidimbu zvakasiyana muma nanometers mashoma kana ma milligrams. Iyi nzira inowanzoshandiswa mu biochemistry uye molecular biology pakuparadzanisa zvikamu zve subcellular, kudzidza kudyidzana kwemapuroteni nemapuroteni, uye kuona huremu hwemamorekuru emacromolecules.

4. Zvinhu Zvinokanganisa Kushanda Zvakanaka kweCentrifugation:

Zvinhu zvakawanda zvinogona kukanganisa kushanda zvakanaka uye kubudirira kwe centrifuge process. Izvi zvinosanganisira kumhanya kwe centrifuge, nguva yekumhanya, tembiricha, uye sarudzo yemachubhu e centrifuge kana ma rotor. Kunzwisisa zvinhu izvi uye kuzvigadzirisa kunogona kuvandudza zvakanyanya mhedzisiro yekuparadzanisa inowanikwa kubva mu centrifuge.

5. Zvakanakira uye Zvisingaitwe neCentrifugation:

Kupinza zvinhu mu centrifugation kune zvakanakira zvakawanda kupfuura dzimwe nzira dzekuparadzanisa. Inzira inokurumidza uye inoshanda zvakasiyana-siyana inogona kubata huwandu hwakawanda hwesampuru uye ine mwero wepamusoro wekudzokorodza. Nzira iyi inogonawo kuwedzerwa nyore nyore kushandiswa mumaindasitiri. Zvisinei, kupinza zvinhu mu centrifugation kunewo zvakusingakwanisi, zvakaita sekukuvadzwa kwesampuru nekumhanya kwakanyanya, kudiwa kwemidziyo yakasarudzika, uye zvipingamupinyi mukuparadzanisa zvidimbu zvine huwandu hwakafanana.

Mhedziso:

Kugadzira zvinhu zvinosanganisa makemikari (centrifugation) inzira yakakosha yekuparadzanisa zvinhu zvakasiyana-siyana zvesainzi nemaindasitiri. Kukwanisa kwayo kuparadzanisa nekuchenesa zvinhu zvichienderana nehuwandu hwazvo kwakachinja tsvakiridzo nekuvandudzwa kwemhando dzakasiyana-siyana dzezvinhu. Nekufambira mberi kuri kuramba kuchiita uye kuvandudzwa kwenzira itsva dzekugadzira zvinhu zvinosanganisa makemikari, nzira iyi ine simba icharamba ichiita basa rakakosha mukuwana zvinhu zvesainzi uye kufambira mberi kwetekinoroji kwemakore anotevera.