Ho arola metsoako ka sekontiri ho arola metsoako joang

Ho Centrifugation: Mokhoa o Matla oa ho Arohanya Metswako

Selelekela:

Ho Centrifugation ke mokhoa o sebelisoang haholo oa laboratori bakeng sa ho arola metsoako ho latela sekhahla sa eona sa ho senngoa ha seretse tšimong ea matla a centrifugal. Mokhoa ona o lumella ho aroloa le ho hloekisoa ha likarolo tse fapaneng ka har'a motsoako, ho kenyeletsoa lintho tse tiileng, metsi, esita le likaroloana tse emisitsoeng ka har'a ho emisoa.

1. Melao-motheo ea Motheo ea ho Centrifugation:

Ho utloisisa hore na ho centrifugation ho sebetsa joang, ho bohlokoa ho utloisisa melao-motheo ea eona ea motheo. Botebong ba eona, ho centrifugation ho itšetlehile ka molao-motheo oa ho sedimentation, o kenyeletsang ho lula ha likaroloana tse teteaneng tlas'a tšusumetso ea matla a khoheli. Ka ho sebelisa matla a centrifugal, a matla ho feta matla a khoheli ka makhetlo a 'maloa, thepa e nang le makhabane a fapaneng e ka aroloa ka katleho.

2. Ho Lokisa Sampole bakeng sa ho Centrifugation:

Pele o etsa centrifugation, ho bohlokoa ho lokisa sampole hantle. Ho latela mofuta oa motsoako o hlahlojoang, mehato e fapaneng ea pele ho centrifugation e ka hlokahala. Sena se ka kenyelletsa ho eketsa tharollo e itseng ea buffer, centrifugation medium, kapa esita le ho phekola sampole pele ho tlosa lintho tse sa batleheng tse ka sitisang ts'ebetso ea karohano.

3. Mefuta ea ho Centrifugation le Litšebeliso tsa eona:

Ho centrifugation ho ka aroloa ka mefuta e fapaneng ho latela lintlha tse kang lebelo, mokhoa, le ts'ebeliso e reriloeng. Mefuta e tloaelehileng haholo ea mekhoa ea karohano ea centrifugal e sebelisoang lilaboratoring e kenyelletsa ho centrifugation e fapaneng, ho centrifugation ea gradient ea density, le ho centrifugation ea ultracentrifugation. A re shebisiseng mefuta ena le lits'ebetso tsa eona tse ikhethileng.

3.1 Phapang ea ho Fokotsa Matla:

Ho arola likaroloana, ho boetse ho tsejoa e le ho arola likaroloana, ke mokhoa o sebelisoang haholo o sebelisetsoang ho arola likarolo tsa boholo bo fapaneng. Mokhoa ona o sebetsa ka ho eketsa butle-butle matla a ho arola likaroloana ho ea ho sediment ka tatellano le ho bokella likaroloana kapa likaroloana ho latela boholo le bongata ba tsona. Mohlala oa ts'ebeliso ea ona ke ho arola li-organelle tsa sele ho tsoa liseleng tse homogenized.

3.2 Ho Fokotsa Botenya ba Gradient:

Ho kopanya di-gradient ka bongata ho kenyelletsa ho beha sampole hodima sebaka se kopanyang di-gradient ka bongata, jwalo ka sucrose kapa cesium chloride. Ha se-centrifuge se ntse se potoloha, dikarolwana tse ka hara sampole di tsamaya ka hara sebaka se kopanyang di-gradient ka bongata ho fihlela di fihla boemong ba tsona ba tekano ho latela bongata ba tsona. Mokgwa ona o thusa haholo bakeng sa ho arola dikarolwana kapa dikarolo tse nang le boholo bo tshwanang empa di na le boima bo fapaneng. O fumana ditshebediso ho hlwekiseng divaerase, diprotheine le di-nucleic acid.

3.3 Ho kenya disenthifere tse ngata ka lebelo le phahameng:

Ultracentrifugation ke mokhoa o matla o sebelisoang ho arola likaroloana ho latela sebopeho sa tsona, boholo le bongata ba tsona. Ka ho sebelisa lebelo le phahameng le linako tse telele tsa ho matha, ultracentrifugation e ka khetholla ka katleho lipakeng tsa likaroloana tse fapaneng ka li-nanometer kapa li-milligram tse 'maloa feela. Mokhoa ona o sebelisoa khafetsa ho biochemistry le biology ea limolek'hule bakeng sa ho arola likaroloana tse ka tlas'a sele, ho ithuta likamano tsa protheine le protheine, le ho fumana boima ba limolek'hule tsa li-macromolecule.

4. Mabaka a Amang Bokgoni ba ho Centrifugation:

Mabaka a 'maloa a ka susumetsa katleho le katleho ea ts'ebetso ea ho centrifugation. Tsena li kenyelletsa lebelo la ho centrifugation, nako ea ho matha, mocheso, le khetho ea lipeipi tsa centrifuge kapa li-rotor. Ho utloisisa lintlha tsena le ho li ntlafatsa ho ka ntlafatsa haholo liphetho tsa karohano tse fumanoeng ho centrifugation.

5. Melemo le Mefokolo ea ho Centrifugation:

Ho centrifugation ho fana ka melemo e 'maloa ho feta mekhoa e meng ea karohano. Ke mokhoa o potlakileng le o feto-fetohang o ka sebetsanang le mefuta e mengata ea lisampole 'me o na le sekhahla se phahameng sa ho ikatisa hape. Mokhoa ona o ka boela oa atolosoa habonolo bakeng sa lits'ebetso tsa indasteri. Leha ho le joalo, ho centrifugation ho boetse ho na le mefokolo ea oona, joalo ka tšenyo ea sampole ka lebelo le phahameng, tlhoko ea lisebelisoa tse khethehileng, le mefokolo ea ho arola likaroloana tse nang le makhakhapha a tšoanang.

Qetello:

Ho thiba metsoako ke mokhoa oa bohlokoa oa ho arola metsoako masimong a fapaneng a saense le a indasteri. Bokhoni ba eona ba ho arola le ho hloekisa lintho ho latela bongata ba tsona bo fetotse lipatlisiso le nts'etsopele ho pholletsa le mefuta e fapaneng ea lithuto. Ka tsoelo-pele e tsoelang pele le nts'etsopele ea mekhoa e mecha ea ho thiba metsoako, mokhoa ona o matla o tla tsoela pele ho bapala karolo ea bohlokoa litšibollong tsa saense le tsoelo-peleng ea theknoloji lilemong tse tlang.