Me pēhea te wehewehe a te centrifugation i ngā ranunga

Te Whakapūngao: He Tikanga Kaha mō te Wehewehe i ngā Ranunga

Kupu Whakataki:

He tikanga taiwhanga whānui te whakamahi i te pūwero hei wehewehe i ngā ranunga i runga i ō rātou tere whakatōtō i roto i te āpure kaha pūwero. Mā tēnei tikanga ka taea te wehe me te pure i ngā wāhanga rerekē o roto i te ranunga, tae atu ki ngā totoka, ngā wai, tae atu ki ngā matūriki e iri ana i roto i te whakawero.

1. Ngā Mātāpono Taketake o te Whakapūngao:

Hei mārama ki te mahi a te centrifugation, he mea nui kia mārama ki ōna mātāpono taketake. Ko te kaupapa matua o te centrifugation ko te whakatōnga, arā, ko te taunga o ngā matūriki matotoru i raro i te mana o te kaha ā-papa. Mā te whakamahi i te kaha centrifugation, he maha ngā wā e kaha ake ana i te kaha ā-papa, ka taea te wehewehe pai i ngā rauemi he rerekē ngā matotoru.

2. Te Whakarite i te Tauira mō te Whakapūngao:

I mua i te mahi i te centrifugation, he mea nui kia tika te whakarite i te tauira. I runga i te āhua o te ranunga e tātarihia ana, me mahi pea ētahi mahi i mua i te centrifugation. Tērā pea ko te tāpiri i tētahi otinga ārai motuhake, te reo centrifugation, te maimoatanga rānei i mua i te tauira hei tango i ngā matū kāore e hiahiatia ana ka whakararuraru i te tukanga wehenga.

3. Ngā Momo Whakapūtanga me ā rātou Whakamahinga:

Ka taea te whakarōpū i te centrifugation ki ngā momo rerekē i runga i ngā āhuatanga pēnei i te tere, te tikanga, me te whakamahinga e whakaarohia ana. Ko ngā momo tikanga wehewehe centrifugation e whakamahia whānuitia ana i roto i ngā taiwhanga ko te centrifugation rerekētanga, te centrifugation pikinga mātotoru, me te ultracentrifugation. Kia tirohia tata atu ēnei momo me ā rātou whakamahinga motuhake.

3.1 Te Whakapūtanga Rerekētanga:

Ko te centrifugation rerekē, e mōhiotia ana ko te centrifugation wehewehenga, he tikanga e whakamahia whānuitia ana hei wehewehe i ngā wāhanga o ngā rahi rerekē. Ka mahi tēnei tikanga mā te whakanui haere i te kaha centrifugal kia whakatōkia, kia kohia ai ngā matūriki, ngā wāhanga rānei i runga i tō rātou rahi me te mātotoru. Ko tētahi tauira o tōna whakamahinga ko te wehe i ngā whekau pūtau mai i ngā pūtau kua whakaranua.

3.2 Te Whakapūtanga o te Rerekētanga Kiato:

Ko te whakapūtanga i te pikinga matotoru ko te whakatakoto i tētahi tauira ki runga i tētahi mea whakapūtanga matotoru, pērā i te sucrose, te cesium chloride rānei. I te hurihuri o te whakapūtanga, ka neke ngā matūriki i roto i te tauira mā roto i te mea whakapūtanga matotoru kia tae rā anō ki tō rātou tūranga taurite i runga i tō rātou matotoru. He tino whai hua tēnei tikanga mō te wehewehe i ngā matūriki, i ngā wāhanga rānei he rite te rahi engari he rerekē te matotoru. Ka kitea ngā tono i roto i te purenga o ngā huaketo, ngā pūmua, me ngā waikawa karihi.

3.3 Te Whakapūngao-ā-Ulu:

He tikanga kaha te ultracentrifugation e whakamahia ana hei wehewehe i ngā matūriki i runga i te āhua, te rahi, me te mātotoru. Mā te whakamahi i ngā tere teitei me ngā wā oma roa, ka taea e te ultracentrifugation te wehewehe pai i waenga i ngā matūriki he rerekē noa iho i roto i ētahi nanometa, i ētahi mirikaramu rānei. He maha ngā whakamahinga o tēnei tikanga i roto i te matū koiora me te koiora ngota hei wehe i ngā wāhanga pūtau-iti, hei ako i ngā taunekeneke pūmua-pūmua, me te whakatau i te taumaha ngota o ngā macromolecules.

4. Ngā Take e Pā Ana ki te Whai Hua o te Whakapūtanga:

He maha ngā āhuatanga ka pā ki te whai huatanga me te angitu o te tukanga centrifugation. Kei roto i ēnei ko te tere o te centrifugation, te roa o te oma, te pāmahana, me te kōwhiringa o ngā ngongo centrifugation, o ngā rotor rānei. Mā te mārama ki ēnei āhuatanga me te arotau i aua āhuatanga ka tino whakapai ake i ngā hua wehenga i puta mai i te centrifugation.

5. Ngā Painga me ngā Here o te Whakapūngao:

He maha ngā painga o te centrifugation i ērā atu tikanga wehewehe. He tikanga tere, he maha hoki ngā whakamahinga, ka taea te whakahaere i te whānuitanga o ngā rahinga tauira, ā, he tere te whakaputa anō. Ka taea hoki te whakanui ake i te tikanga mō ngā tono ahumahi. Heoi, he iti anō hoki ngā herenga o te centrifugation, pērā i te kino o te tauira i ngā tere teitei, te hiahia mō ngā taputapu motuhake, me ngā herenga ki te wehewehe i ngā matūriki he rite te matotoru.

Whakatau:

He tikanga nui te whakamahi i te pūwero hei wehewehe i ngā ranunga i roto i ngā momo mara pūtaiao me ngā mara ahumahi. Nā tōna kaha ki te wehe me te pure i ngā matū i runga i ō rātou matotoru kua huri te rangahau me te whanaketanga puta noa i ngā momo mara. Mā te haere tonu o ngā whanaketanga me te whanaketanga o ngā huarahi pūwero hou, ka whai wāhi nui tonu tēnei tikanga kaha ki ngā kitenga pūtaiao me ngā whanaketanga hangarau mō ngā tau e haere ake nei.