ingabe usawoti uhlukana namanzi uma ku-centrifuge

Ingabe Usawoti Uhlukana Namanzi Uma Ufakwa Emkhathini?

Isingeniso

I-Centrifugation iyindlela evame ukusetshenziswa emalabhorethri nasezimbonini ukuhlukanisa izingxube ngokusekelwe ekuminyaneni kwazo. Kuhilela ukuphotha ingxube ngesivinini esikhulu kusetshenziswa umshini we-centrifuge, okhiqiza amandla e-centrifugal. La mandla abangela ukuthi izingxenye ezahlukene zengxube zihlukane ngenxa yobukhulu bazo obuhlukahlukene. Kulesi sihloko, sihlola umbuzo othi, "Ingabe usawoti uyahlukana namanzi uma ufakwa i-centrifuge?" futhi sixoxe ngezimiso ezingemuva kokuhlukaniswa kwamanzi anosawoti ngokusebenzisa i-centrifugation.

Ukuqonda i-Centrifugation

1. Isimiso Sokugqamisa I-Centrifugation

I-Centrifugation isebenza ngesimiso se-sedimentation, okuyinqubo yokuhlalisa phansi kwezinhlayiya ngenxa yamandla adonsela phansi. Lapho umshini we-centrifuge ujikeleza ingxube ngesivinini esikhulu, ukhiqiza amandla e-centrifugal alingisa umphumela wamandla adonsela phansi. La mandla abangela ukuthi izinhlayiya eziqinile zihambe ziye phansi kuyilapho izinto ezilula zihlala zilenga noma zihlala phezulu. Ngokudala la ma-density gradient, singahlukanisa ngempumelelo izingxenye ezahlukene zengxube.

2. Usawoti Namanzi: Ubuningi Obuhlukene

Usawoti namanzi anobukhulu obuhlukene, okwenza kube yizinto ezifanele ukuhlukaniswa ngokusebenzisa i-centrifugation. Usawoti, njengoba uqinile, unzima kunamanzi, okuyi-liquid. Ngenxa yalo mehluko wobuningi, izinhlayiya zikasawoti zizohamba ziye phansi ngesikhathi se-centrifugation, kuyilapho amanzi ezohlala eduze kwaphezulu.

Inqubo Yokuhlola

Ukuze uhlole ukuthi usawoti uyahlukana yini namanzi uma ufakwa ku-centrifuge, landela lezi zinyathelo:

Isinyathelo 1: Lungisa Ingxube Yamanzi Anosawoti

Thatha i-beaker bese ufaka inani elithile lamanzi kuyo. Faka kancane kancane usawoti e-beaker bese uvuselela isixazululo ukuqinisekisa ukuxubana okufanele. Landelela inani likasawoti namanzi asetshenziswayo ukugcina ukuhlushwa okuthile.

Isinyathelo 2: Dlulisa Ingxube Kumapayipi E-Centrifuge

Thela ingxube yamanzi anosawoti ngokucophelela emapayipini e-centrifuge anobukhulu obufanele. Qinisekisa ukuthi amapayipi alinganiswe kahle futhi aboshwe kahle ukuze kugwenywe noma yiziphi izingozi ngesikhathi se-centrifuge.

Isinyathelo 3: Faka i-Centrifuge ku-Mixture

Faka amapayipi emshinini we-centrifuge bese usetha isivinini nesikhathi osifunayo sokuhlola. Qala i-centrifuge bese uyivumela ukuthi ijikeleze ngesikhathi esibekiwe.

Isinyathelo 4: Qaphela Imiphumela

Ngemva kokuthi i-centrifuge isiqediwe, susa ngokucophelela amapayipi emshinini bese ubheka izingxenye ezihlukanisiwe. Cishe uzobona ungqimba olucacile oluqukethe amanzi phezulu, kuyilapho usawoti ubuzohlala phansi kwamapayipi e-centrifuge.

Ingxoxo kanye Nezicelo

3. Ukufakwa kwe-Centrifugation ekukhiqizweni kukasawoti

Ukufakwa kwe-centrifugation kudlala indima ebalulekile ekukhiqizweni kukasawoti, lapho kusiza khona ekuhlukaniseni ukungcola nokuthola amakristalu kasawoti asezingeni eliphezulu. Ngemva kokukhishwa kokuqala kukasawoti emanzini olwandle noma ezimayini ezingaphansi komhlaba, kungase kube nokungcola okufana nesihlabathi, ubumba, kanye nezinye izimbiwa. Ukufakwa kwe-centrifugation kusiza ukuhlukanisa lokhu kungcola nesixazululo sikasawoti, okuholela ekhwalithini engcono yomkhiqizo.

4. Izicelo kuCwaningo Lwezinto Eziphilayo

I-Centrifugation isetshenziswa kabanzi ocwaningweni lwezinto eziphilayo ukuze kuhlukaniswe izingxenye ezahlukene zamaseli ngokusekelwe ekuminyaneni kwazo. Le ndlela ivumela ososayensi ukuthi bahlukanise ama-organelle athile amaseli, amaprotheni, kanye ne-DNA ngokudala i-density gradients. Isibonelo, ocwaningweni lwegazi, i-centrifugation ihlukanisa amaseli abomvu egazi ku-plasma, okwenza kube lula ukuhlaziya okwengeziwe kanye nokuxilongwa kwezifo ezahlukahlukene.

Isiphetho

I-Centrifugation iyindlela enamandla yokuhlukanisa izingxube ngokusekelwe kuma-density gradients, futhi ingahlukanisa ngempumelelo usawoti emanzini. Ngokusebenzisa isimiso se-sedimentation, izinhlayiya zikasawoti ezixineneyo zihlala phansi ngesikhathi se-centrifugation kuyilapho amanzi ehlala phezulu. Lokhu kuhlukaniswa kubalulekile emikhakheni ehlukahlukene, njengokukhiqizwa kukasawoti kanye nocwaningo lwezinto eziphilayo, lapho ukuthola izingxenye ezihlanzekile kubalulekile khona. Ngakho-ke, impendulo yombuzo othi, "Ingabe usawoti uyahlukana namanzi uma u-centrifuged?" inguyebo ozwakalayo.