Na letsoai le arohana le metsi ha ho centrifuge

Na Letswai le Arohane le Metsi ha le Sebelisoa ka Seterifu?

Selelekela

Ho centrifugation ke mokhoa o sebediswang haholo dilaboratoring le diindastering ho arola metswako ho latela bongata ba yona. E kenyeletsa ho phutholla motswako ka lebelo le phahameng ho sebediswa mochini wa centrifuge, o hlahisang matla a centrifuge. Matla ana a etsa hore dikarolo tse fapaneng tsa motswako di arohane ka lebaka la bongata ba tsona bo fapaneng. Sehloohong sena, re hlahloba potso e reng, "Na letswai le arohana le metsi ha le centrifugation?" mme re buisana ka melaomotheo e ka mora karohano ya metsi a letswai ka ho centrifugation.

Ho utloisisa Centrifugation

1. Molao-motheo oa Centrifugation

Ho centrifugation ho sebetsa hodima molao-motheo wa ho sedimentation, e leng tshebetso ya ho dula ha dikarolwana ka lebaka la matla a kgoheli. Ha mochini wa ho centrifuga o potoloha motswako ka lebelo le phahameng, o hlahisa matla a ho centrifuga a etsisang phello ya matla a kgoheli. Matla ana a etsa hore dikarolwana tse teteaneng di tsamaye ho ya tlase ha dikarolo tse bobebe di ntse di leketlile kapa di dula hodimo. Ka ho theha di-gradients tsena tsa bongata, re ka arola dikarolo tse fapaneng tsa motswako ka katleho.

2. Letswai le Metsi: Bongata bo Fapaneng

Letswai le metsi di na le botenya bo fapaneng, e leng se etsang hore e be bakhethoa ba loketseng ho arolwa ka ho centrifugation. Letswai, kaha ke ntho e tiileng, le teteaneng ho feta metsi, e leng mokelikeli. Ka lebaka la phapang ena ya botenya, dikarolwana tsa letswai di tla tsamaya ho ya tlase nakong ya centrifugation, ha metsi a tla dula a le haufi le karolo e ka hodimo.

Mokhoa oa Teko

Ho leka hore na letsoai le arohana le metsi ha le kenngoa ka seterifung, latela mehato ena:

Mohato oa 1: Lokisa Motsoako oa Metsi a Letsoai

Nka beaker 'me u kenye metsi a itseng ho eona. Kenya letsoai butle-butle beaker 'me u hlohlelletse tharollo ho netefatsa hore e tsoakoa hantle. Boloka tlaleho ea bongata ba letsoai le metsi a sebelisitsoeng ho boloka khatello e itseng.

Mohato oa 2: Fetisetsa Motsoako ho Li-Centrifuge Tubes

Tšela motsoako oa metsi a letsoai ka hloko ka har'a liphaephe tsa centrifuge tse boholo bo loketseng. Netefatsa hore liphaephe li leka-lekane hantle 'me li tlamiloe ka thata ho qoba likotsi leha e le life nakong ea ho centrifuge.

Mohato oa 3: Ho Seta Motsoako ka Seterifu

Beha lipeipi ka har'a mochini oa centrifuge 'me u behe lebelo le nako e lakatsehang bakeng sa teko. Qala centrifuge 'me u e lumelle ho potoloha ka nako e behiloeng.

Mohato oa 4: Hlokomela Liphetho

Kamora hore ho centrifugation ho phethehe, tlosa lipeipi ka hloko mochining 'me u shebe likarolo tse arohaneng. Mohlomong u tla bona lera le hlakileng le nang le metsi holimo, ha letsoai le ne le tla lula tlase lipeiping tsa centrifuge.

Puisano le Litšebeliso

3. Ho Centrifugation Tlhahisong ea Letsoai

Ho ntsha letsoai ka sekontiri ho bapala karolo ea bohlokoa tlhahisong ea letsoai, moo ho thusang ho arola litšila le ho fumana likristale tsa letsoai tsa boleng bo holimo. Kamora ho ntša letsoai la pele metsing a leoatle kapa merafong e ka tlas'a lefatše, litšila tse kang lehlabathe, letsopa le liminerale tse ling li ka ba teng. Ho ntsha letsoai ka sekontiri ho thusa ho arola litšila tsena ho tsoa tharollong ea letsoai, e leng se lebisang boleng bo betere ba sehlahisoa.

4. Likopo tsa Lipatlisiso tsa Baeloji

Ho centrifugation ho sebediswa haholo dipatlisisong tsa baeloji ho arola dikarolo tse fapaneng tsa sele ho latela bongata ba tsona. Mokgwa ona o dumella borasaense ho arola di-organelle tse itseng tsa sele, diprotheine le DNA ka ho theha di-gradient tsa bongata. Mohlala, thutong ya madi, ho centrifugation ho arola disele tse kgubedu tsa madi ho plasma, ho nolofalletsa tlhahlobo e eketsehileng le ho fumana mafu a fapaneng.

Qetello

Ho centrifugation ke mokhoa o matla oa ho arola metsoako ho latela maemo a teteaneng, 'me e ka arola letsoai ho metsi ka katleho. Ka ho sebelisa molao-motheo oa ho sedimentation, likaroloana tsa letsoai tse teteaneng li lula tlase nakong ea ho centrifugation ha metsi a ntse a le holimo. Karohano ena e bohlokoa haholo masimong a fapaneng, joalo ka tlhahiso ea letsoai le lipatlisiso tsa baeloji, moo ho fumana likarolo tse hloekileng ho leng bohlokoa. Kahoo, karabo ea potso e reng, "Na letsoai le arohana le metsi ha le centrifuged?" ke e e e utloahalang.