munyu unoparadzaniswa nemvura here kana uchitenderera

Munyu Unoparadzaniswa Nemvura Here Kana Wakaiswa Mukati?

Nhanganyaya

Kupinza musanganiswa (centrifugation) inzira inoshandiswa zvakanyanya mumarabhoritari nemaindasitiri kupatsanura musanganiswa zvichienderana nekuwanda kwawo. Izvi zvinosanganisira kutenderedza musanganiswa nekumhanya kwakanyanya uchishandisa muchina we centrifuge, uyo unogadzira simba re centrifugal. Simba iri rinoita kuti zvikamu zvakasiyana zvemusanganiswa zviparadzane nekuda kwekuwanda kwazvo kwakasiyana. Muchinyorwa chino, tinoongorora mubvunzo wekuti, "Munyu unoparadzana nemvura here kana uchiiswa mu centrifuge?" uye tinokurukura misimboti iri shure kwekupatsanurwa kwemunyu nemvura kuburikidza ne centrifugation.

Kunzwisisa Centrifugation

1. Nheyo yeCentrifugation

Kutenderera kunoshanda panheyo yekudzika kwevhu, inova nzira yekudzika kwezvikamu nekuda kwegiravhiti. Kana muchina we centrifuge uchitenderedza musanganiswa nekumhanya kwakanyanya, unogadzira simba re centrifugal rinotevedzera mhedzisiro yegiravhiti. Simba iri rinoita kuti zvikamu zvakawandisa zvifambe zvichienda pasi nepo zvinhu zvakareruka zvichiramba zvakamira kana kugara pamusoro. Nekugadzira aya ma gradients e density, tinogona kuparadzanisa zvinobudirira zvikamu zvakasiyana zvemusanganiswa.

2. Munyu neMvura: Kuwanda kwakasiyana

Munyu nemvura zvine huwandu hwakasiyana, izvo zvinoita kuti zvive zvakanaka pakupatsanurwa kuburikidza nekutenderera. Munyu, sezvo uri chinhu chakasimba, wakaoma kupfuura mvura, inova mvura. Nekuda kwemusiyano uyu wekutenderera, zvidimbu zvemunyu zvinofamba zvichienda pasi panguva yekutenderera, nepo mvura ichiramba iri pedyo nepamusoro.

Maitiro Ekuyedza

Kuti uedze kana munyu uchiparadzana nemvura kana waiswa mu centrifuge, tevera matanho aya:

Danho 1: Gadzirira Musanganiswa weMunyu neMvura

Tora bhodhoro remvura woisa mvura yakati wandei mariri. Wedzera munyu zvishoma nezvishoma mubhodhoro wokurunga mvura yacho kuti uve nechokwadi chekusanganisa zvakanaka. Ramba uchitarisa huwandu hwemunyu nemvura zvinoshandiswa kuchengetedza huwandu hwakati.

Danho rechipiri: Tumira Musanganiswa kuCentrifuge Tubes

Dururirai musanganiswa wemvura ine munyu nemazvo mumachubhu e centrifuge akakura zvakakodzera. Iva nechokwadi chekuti machubhu acho akaenzana zvakanaka uye akabatana zvakanaka kudzivirira tsaona panguva ye centrifuge.

Danho rechitatu: Kuisa musanganiswa pakati pema "centrifuge"

Isa machubhu mumuchina we centrifuge woisa kumhanya nenguva yaunoda yekuedza. Tanga centrifuge woirega ichitenderera panguva yakatarwa.

Danho rechina: Tarisa Mhedzisiro

Kana centrifugation yapera, bvisa machubhu acho nokungwarira uye tarisa zvikamu zvakaparadzana. Ungangoona jira rakajeka rine mvura pamusoro, nepo munyu wacho waizogara pasi pechubhu dzecentrifuge.

Kukurukurirana uye Mashandisirwo

3. Kugadzira Munyu muCentrifugation

Kugadzira munyu uchishandisa centrifugation kunoita basa guru mukugadzira munyu, uko kunobatsira mukuparadzanisa tsvina uye kuwana makristaro emunyu emhando yepamusoro. Mushure mekutorwa kwemunyu kubva mumvura yegungwa kana pasi pevhu, tsvina yakaita sejecha, ivhu, nezvimwe zvicherwa inogona kunge iripo. Kugadzira munyu uchishandisa centrifugation kunobatsira kuparadzanisa tsvina iyi kubva mumunyu, zvichiita kuti chigadzirwa chive nani.

4. Mashandisirwo muKutsvagurudza Kwezvinhu Zvipenyu

Kupinza ropa mumasero (centrifugation) kunoshandiswa zvakanyanya mukutsvagisa kwezvehupenyu kupatsanura zvikamu zvakasiyana zvemasero zvichienderana nekuwanda kwazvo. Iyi nzira inobvumira masayendisiti kupatsanura ma organelles emasero chaiwo, mapuroteni, uye DNA nekugadzira density gradients. Semuenzaniso, mukudzidza ropa, kupinza ropa mumasero kunoparadzanisa masero matsvuku eropa kubva muplasma, zvichiita kuti pave nekuongororwa kwakawanda uye kuongororwa kwezvirwere zvakasiyana-siyana.

Mhedziso

Kugadzira centrifugation inzira ine simba yekuparadzanisa musanganiswa zvichibva pahuwandu hwe density gradients, uye inogona kuparadzanisa munyu nemvura zvinobudirira. Nekushandisa musimboti we sedimentation, zvidimbu zvemunyu zvinogara pasi panguva ye centrifugation nepo mvura ichiramba iri pamusoro. Kupatsanurana uku kwakakosha munzvimbo dzakasiyana-siyana, dzakadai sekugadzira munyu uye ongororo yezvehupenyu, uko kuwana zvikamu zvakachena kwakakosha. Saka, mhinduro yemubvunzo wekuti, "Munyu unoparadzaniswa nemvura here kana waiswa centrifuge?" ihongu yakasimba.