ka wehea te tote i te wai i te wā e whakamahia ana te centrifuge
Ka wehea te tote i te wai ina hurihia ki te porotītaha?
Kupu Whakataki
Ko te whakamahi i te pūwero he tikanga e whakamahia whānuitia ana i roto i ngā taiwhanga me ngā ahumahi hei wehewehe i ngā ranunga i runga i ō rātou matotoru. Ko te huri i te ranunga i ngā tere teitei mā te whakamahi i te mīhini pūwero, e whakaputa ana i te kaha pūwero. Mā tēnei kaha ka wehea ngā wāhanga rerekē o te ranunga nā te rerekētanga o ō rātou matotoru. I roto i tēnei tuhinga, ka tūhuratia te pātai, "Ka wehea te tote mai i te wai ina pūwerohia?" ā, ka matapakihia ngā mātāpono kei muri i te wehenga tote-wai mā te pūwero.
Te Mārama ki te Whakapūngao
1. Te Mātāpono o te Whakapūtanga
Ko te mahi a te centrifugation i runga i te kaupapa o te whakatōputanga, arā, te tukanga o te taunga o ngā matūriki nā te kaha o te kaha ā-papatipu. Ina hurihia e te mīhini centrifugation te ranunga i ngā tere teitei, ka puta he kaha centrifugation e whai ana i te pānga o te kaha ā-papatipu. Mā tēnei kaha ka neke ngā matūriki matotoru ki raro, ko ngā mea māmā ake ka noho iri, ka tau rānei ki runga. Mā te waihanga i ēnei pikinga kiato, ka taea e tātou te wehewehe i ngā wāhanga rerekē o te ranunga.
2. Te Tote me te Wai: Ngā Matū Rerekē
He rerekē ngā mātotoru o te tote me te wai, ā, he pai rawa atu ēnei mō te wehewehe mā te whakamahi i te centrifugation. He totoka te tote, engari he mātotoru ake i te wai, he wai. Nā tēnei rerekētanga mātotoru, ka neke ngā matūriki tote ki raro i te wā e centrifugation ana, ko te wai ia ka noho tata ki runga.
Tukanga Whakamātautau
Hei whakamātautau mēnā ka wehe te tote i te wai ina hurihia, whai i ēnei mahi:
Hipanga 1: Whakaritea te Ranunga Tote-Wai
Tangohia he kapurete ka tāpirihia he nui o te wai ki roto. Tāpirihia mārire te tote ki roto i te kapurete ka whakaohokia te otinga kia pai ai te whakaranu. Aroturukihia te nui o te tote me te wai e whakamahia ana hei pupuri i tētahi kukū motuhake.
Hipanga 2: Whakawhitihia te Ranunga ki ngā Ngongo Pūwero
Ringihia marietia te ranunga wai tote ki roto i ngā ngongo pūwero e tika ana te rahi. Me whakarite kia taurite pai ngā ngongo, kia mau hoki te here kia kore ai e puta he aituā i te wā o te pūwero.
Hipanga 3: Whakakotahitia te Ranunga
Whakanohoia ngā ngongo ki roto i te mīhini whakapūkara, ka whakatakoto i te tere me te wā e hiahiatia ana mō te whakamātautau. Tīmatahia te whakapūkara, ā, tukua kia huri mō te wā kua tohua.
Hipanga 4: Tirohia ngā Hua
Kia oti te whakapūtā, tangohia marietia ngā ngongo mai i te mīhini, ka mātakitaki i ngā wāhanga kua wehea. Tērā pea ka kite koe i tētahi paparanga mārama kei roto he wai i te tihi, ko te tote ia kua tau ki raro o ngā ngongo whakapūtā.
Kōrero me ngā Whakamahinga
3. Te Whakapūngao i roto i te Hanga Tote
He mea nui te mahi a te pūwero i roto i te hanga tote, ā, ka āwhina i te wehewehe i ngā poke me te whiwhi i ngā tioata tote kounga teitei. Whai muri i te tangohanga tuatahi o te tote mai i te wai moana, i ngā maina whenua rānei, tera pea ka kitea ngā poke pēnei i te onepu, te uku, me ētahi atu kohuke. Ka āwhina te pūwero ki te wehewehe i ēnei poke mai i te wai tote, ka pai ake te kounga o te hua.
4. Ngā Whakamahinga i roto i te Rangahau Koiora
He whānuitia te whakamahinga o te centrifugation i roto i ngā rangahau koiora hei wehewehe i ngā wāhanga pūtau rerekē i runga i ō rātou kiato. Mā tēnei tikanga ka taea e ngā kaipūtaiao te wehewehe i ngā whekau pūtau motuhake, ngā pūmua, me te DNA mā te waihanga i ngā pikinga kiato. Hei tauira, i roto i te rangahau toto, ka wehea e te centrifugation ngā pūtau toto whero mai i te plasma, ka taea ai te tātaritanga me te tautuhi i ngā mate maha.
Whakamutunga
He tikanga kaha te whakamahi i te pūwero hei wehewehe i ngā ranunga i runga i ngā pikinga mātotoru, ā, ka taea te wehewehe i te tote mai i te wai. Mā te whakamahi i te kaupapa o te whakatō, ka tau ngā matūriki tote matotoru ki raro i te wā e pūwero ana, ko te wai kei runga tonu. He mea nui tēnei wehenga i roto i ngā momo mara, pērā i te hanga tote me te rangahau koiora, he mea nui te whiwhi i ngā wāhanga parakore. Nō reira, ko te whakautu ki te pātai, "Ka wehe te tote mai i te wai ina pūwerohia?" he āe tino kaha.
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