Molao-motheo oa karohano o sebelisoang ho centrifugation ke ofe?

Ho Centrifugation: Melao-motheo ea Karohano le Litšebeliso

Selelekela

Ho kopanya metsoako ka sekontiri ke mokhoa o sebelisoang haholo bakeng sa ho arola metsoako ka likarolo tsa eona ho latela bongata ba eona. Mokhoa ona o matla o na le melao-motheo e thehiloeng fisiks ea matla a sekontiri 'me o sebelisoa haholo masimong a fapaneng a saense, bongaka le indasteri. Sehloohong sena, re tla hlahloba molao-motheo oa karohano o sebelisoang ho kopanya metsoako ka botlalo, re totobatsa lits'ebetso le melemo ea oona.

Ho utloisisa Centrifugation

Ho centrifugation ho sebetsa ho latela molao-motheo oa hore likaroloana tse motsoakong o nang le makhabane a fapaneng li tla qhalana ka lebelo le fapaneng ha li le tlas'a matla a phahameng a centrifugal. Ka ho potoloha motsoako ka lebelo le phahameng, likarolo tse teteaneng li ea tlase ha tse bobebe li lula holimo, e leng se lumellang karohano ea tsona. Ts'ebetso ena e itšetlehile ka litšobotsi tsa ho potlakisa ha centripetal, e leng matla a sebetsang ka ntle ho ntho e tsamaeang tseleng e chitja.

Molao-motheo oa 1: Phapang ea ho Fokotsa ho Fapana

Ho potoloha ha differential ke mokhoa o sebediswang haholo baelojing le biochemistry. Ho kenyeletsa mekhahlelo e mengata ya ho potoloha ha di-centrifugation ka lebelo le fapaneng ho arola dikarolo tsa boholo le boima bo fapaneng ka tatellano. Ho potoloha ha pele ho etswa ka lebelo le tlase ho tlosa ditshila tse kgolo le disele, mme ho potoloha ho latelang ka lebelo le phahameng ho arola di-organelle tse nyane kapa dimolek'hule. Ka ho fetola matla a centrifugal le nako bakeng sa ho potoloha ka ho hong le ho hong, mefuta e mengata ya boholo ba dikarolwana e ka arolwa ka katleho.

Molao-motheo oa 2: Ho Fokotsa Botenya ba Gradient

Ho centrifugation ya gradient e teteaneng ke mokhoa o tswetseng pele haholo o kopanyang differential centrifugation le tshebediso ya density gradient medium. Density gradient medium e na le tharollo e nang le density e eketsehang butle-butle ho tloha hodimo ho ya tlase ho tube. Ha sampole e kenngwa hodima gradient mme e sothwa, dikarolwana di falla ka hara medium ho latela density ya tsona e phaphametseng. Mokgwa ona o thusa haholo bakeng sa ho arola di-macromolecule le dikarolo tse ka tlase ho sele tse nang le dipharologanyo tse nyane tsa density.

Tšebeliso ea 1: Karolo ea Protheine

Ho arola liprotheine ka sekontiri ho phetha karolo ea bohlokoa ho aroleng liprotheine, e leng ts'ebetso e sebelisoang libakeng tse fapaneng tsa lipatlisiso, ho kenyeletsoa le liprotheine. Ka ho sebelisa ho arola li-differential centrifugation, hoa khoneha ho arola lisele ho tloha setsing sa temo, ho arola li-organelle, kapa ho arola li-lysate tsa sele kaofela ho fumana liprotheine tse itseng tse khahlisang. Mabelo a phahameng a fihlellehang nakong ea ho arola liprotheine a lumella karohano e sebetsang hantle, ho netefatsa hore na liprotheine li tsebahatsoa joang le hore na li hlahlojoe hantle hakae.

Tšebeliso ea 2: Karohano ea Lisele tsa Mali

Ho centrifugation ho sebediswa haholo dilaboratoring tsa bongaka bakeng sa karohano ya dikarolo tsa madi. Ka ho potoloha disampole tsa madi ka hara diphaephe tse ikgethang tsa centrifuge, disele tse kgubedu tsa madi, disele tse tshweu tsa madi le diplatelete di ka arolwa ka katleho ho tswa ho plasma. Mokgwa ona o bohlokwa bakeng sa merero ya ho hlahloba, kaha o dumella ho sekaseka dikarolo tse itseng tsa madi, tse kang ho bala disele tse tshweu tsa madi kapa ho fumana hore na dikhemikhale kapa dimolek'hule tse itseng di na le bokae.

Molao-motheo oa 3: Ho lokisa Ultracentrifugation

Ho lokisa ultracentrifugation ke mokhoa o sebelisang ultracentrifuges, o khonang ho fihlella lebelo le phahameng ka mokhoa o makatsang, ho arola le ho arola likaroloana le limolek'hule ka tekanyo ea ho lokisa. Mokhoa ona o sebelisoa haholo ho hloekiseng livaerase, li-nucleic acid le liprotheine tse kholo tse rarahaneng. Ka ho khetha ka hloko maemo a centrifugation, joalo ka mofuta oa rotor, lebelo le nako, bafuputsi ba ka arola le ho khutlisa lintho ka katleho bakeng sa ho ithuta kapa ho sebelisa tse ling.

Kopo ea 3: Tlhahlobo ea Tikoloho

Ho centrifugation ho fumana dikopo tsa bohlokwa tlhahlobong ya disampole tsa tikoloho. Mohlala, dibakeng tsa kalafo ya metsi a ditshila, ho centrifugation ho thusa ho arola le ho ntsha dintho tse tiileng metsing, ho netefatsa hore metsi a hlwekileng a a ntshwa. Ka ho tshwanang, tlhahlobong ya mobu le seretse, ho centrifugation ho thusa ho arola dikarolwana le ho ntsha dikarolo tse fapaneng, ho tlatsetsa kutlwisisong ya kabo ya tshilafatso le bophelo bo botle ba tikoloho.

Qetello

Ho centrifugation ke mokhoa o feto-fetohang o fetotseng mekhoa ea karohano mafapheng a mangata a saense. Ka ho utloisisa melao-motheo e ka morao ho ho centrifugation le ho fetola mokhoa ona ho latela lits'ebetso tse itseng, bo-rasaense le bafuputsi ba ka arola metsoako e rarahaneng ka katleho likarolong tsa bona ka bomong. Ho tloha ho aroloeng ha protheine ho ea ho karohano ea lisele tsa mali le tlhahlobo ea tikoloho, ho centrifugation e ntse e le sesebelisoa sa bohlokoahali, se nolofalletsang litšibollo tse ncha le tsoelo-pele mafapheng a fapaneng.