chii chinonzi musimboti wekuparadzanisa unoshandiswa mu centrifugation

Kuchengetedza: Nheyo dzekuparadzanisa uye mashandisirwo

Nhanganyaya

Kupinza zvinhu mu "centrifugation" inzira inowanzoshandiswa pakupatsanura musanganiswa muzvikamu zvawo zvichienderana nehuwandu hwazvo. Nzira iyi ine simba ine misimboti yakadzika midzi mufizikisi yesimba re "centrifugal" uye inoshandiswa zvakanyanya muzvikamu zvakasiyana-siyana zvesainzi, zvokurapa, uye zvemaindasitiri. Muchinyorwa chino, tichaongorora musimboti wekuparadzanisa unoshandiswa mukuisa zvinhu mu "centrifugation" zvakadzama, tichiratidza mashandisirwo azvo uye mabhenefiti azvo.

Kunzwisisa Centrifugation

Kutenderera kunoshanda nepfungwa yekuti zvidimbu zviri mumusanganiswa une huwandu hwakasiyana hwezvinhu zvinodonha nemazinga akasiyana kana zvaiswa simba guru repakati. Nekutenderera musanganiswa nekumhanya kwakanyanya, zvikamu zvakakora zvinofamba zvichienda pasi nepo zvakareruka zvichiramba zviri pamusoro, zvichibvumira kuparadzaniswa kwazvo. Maitiro aya anoenderana nehunhu hwekumhanya kwepakati, inova simba rinoshanda richibuda kunze pane chinhu chinofamba munzira yakatenderera.

Nheyo 1: Kusiyanisa Kwekudzora Kutenderera

Kusiyanisa centrifugation inzira inoshandiswa zvakanyanya mu biology ne biochemistry. Inosanganisira kutenderera kwakawanda kwe centrifugation nekumhanya kwakasiyana kuti zvikamu zvehukuru hwakasiyana uye densities zviparadzaniswe zvakatevedzana. Kutenderera kwekutanga kunoitwa nekumhanya kwakaderera kubvisa marara makuru nemasero, nekutenderera kunotevera nekumhanya kwakakwirira kuti zviparadzanise organelles diki kana mamorekuru. Nekugadzirisa simba re centrifugal nenguva yekutenderera kwega kwega, huwandu hwakawanda hwezvimedu zvinogona kupatsanurwa zvinobudirira.

Nheyo yechipiri: Kudzora Kureba Kwemagiraidhi

Kuyera density gradient centrifugation inzira yepamusoro inosanganisa differential centrifugation nekushandisa density gradient medium. Density gradient medium ine mhinduro ine density inowedzera zvishoma nezvishoma kubva kumusoro kusvika pasi pechubhu. Kana sampuro yaiswa pamusoro pe gradient yozopotwa, zvidimbu zvinofamba nepakati zvichienderana ne density yazvo inoyerera. Iyi nzira inonyanya kubatsira pakuparadzanisa macromolecules nezvikamu zve subcellular zvine musiyano mudiki mu density.

Kushanda 1: Kupatsanura Mapuroteni

Kuparadzanisa mapuroteni kunoita basa guru mukupatsanura mapuroteni, nzira inoshandiswa munzvimbo dzakasiyana-siyana dzekutsvagisa, kusanganisira mapuroteni. Nekushandisa differential centrifugation, zvinokwanisika kuparadzanisa masero kubva kune culture medium, kupatsanura maorganelles, kana kupatsanura masero ese kuti uwane mapuroteni chaiwo anodiwa. Kumhanya kwakanyanya kunowanikwa panguva yekupatsanura mapuroteni kunobvumira kupatsanurana kwakanaka, zvichiita kuti mapuroteni aonekwe nemazvo uye aongororwe nemazvo.

Kushanda 2: Kuparadzaniswa Kwemasero Eropa

Kutenderera ropa kunoshandiswa zvakanyanya mumarabhoritari ekiriniki ekuparadzanisa zvikamu zveropa. Nekutenderedza sampuli dzeropa mumachubhu e centrifuge akasarudzika, masero matsvuku eropa, masero machena eropa, uye maplatelet zvinogona kuparadzaniswa zvinobudirira kubva muplasma. Iyi nzira yakakosha pakuongorora, sezvo ichibvumira kuongororwa kwezvikamu zveropa, zvakaita sekuverenga masero machena eropa kana kuona huwandu hwemakemikari kana mamorekuru.

Nheyo 3: Preparative Ultracentrifugation

Kugadzirira ultracentrifugation inzira inoshandisa ultracentrifuges, inokwanisa kusvika pakumhanya kwakanyanya, kuparadzanisa nekuparadzanisa zvidimbu nemamorekuru pachiyero chekugadzirira. Iyi nzira inoshandiswa zvakanyanya mukuchenesa mavhairasi, nucleic acids, uye mapuroteni makuru. Nekusarudza nekungwarira mamiriro e centrifugation, akadai serudzi rwe rotor, kumhanya, uye nguva, vaongorori vanogona kuparadzanisa zvinobudirira uye kudzoreredza zvinhu kuti zviongororwe kana kushandiswa zvakanyanya.

Chishandiso 3: Kuongorora Zvakatipoteredza

Kuchenesa mvura kubva muvhu (centrifugation) kunobatsira zvikuru pakuongorora nharaunda. Semuenzaniso, mumiti yekuchenesa mvura yakasviba, kusvina mvura kubva muvhu kunobatsira pakuparadzanisa nekubvisa zvinhu zvakaoma mumvura, zvichiita kuti mvura ibude yakachena. Saizvozvowo, pakuongorora ivhu nematope, kusvina mvura kubva muvhu kunobatsira pakuparadzanisa zvidimbu uye kubvisa zvinhu zvakasiyana-siyana, zvichibatsira pakunzwisisa kupararira kwezvinosvibisa uye hutano hwenzvimbo.

Mhedziso

Kugadzira centrifugation inzira inoshanda zvakasiyana-siyana yakachinja maitiro ekuparadzanisa muzvikamu zvakasiyana-siyana zvesainzi. Nekunzwisisa misimboti iri shure kwekugadzira centrifugation uye kugadzirisa nzira iyi kuti ienderane nemashandisirwo chaiwo, masayendisiti nevaongorori vanogona kuparadzanisa zvinobudirira musanganiswa wakaomarara muzvikamu zvavo. Kubva pakupatsanurwa kwemapuroteni kusvika pakupatsanurwa kwemasero eropa uye kuongororwa kwezvakatipoteredza, kugadzira centrifugation kunoramba kuri chishandiso chakakosha, zvichigonesa kuwana zvinhu zvitsva uye kufambira mberi munzvimbo dzakasiyana-siyana.