mokhoa oa ho arola ka centrifugation ke eng

Mokhoa oa ho Arohanya ka Sekhahla: Ho Fetola Mekhoa ea Laboratori

Selelekela

Lefatšeng la lipatlisiso tsa mahlale le mekhoa ea laboratori, mekhoa ea karohano e bapala karolo ea bohlokoa ho aroleng likarolo tse itseng ho tsoa metswakong e rarahaneng. Leha ho na le mekhoa e 'maloa e fumanehang, ho sebelisa centrifugation ho hlahile e le e 'ngoe ea mekhoa e sebetsang hantle le e sebelisoang haholo. Sengoloa sena se hlahloba molao-motheo o ka morao ho ho sebelisa centrifugation le lits'ebeliso tsa eona tse fapaneng masimong a fapaneng a mahlale.

Ho utloisisa Centrifugation

Ho Centrifugation ke mokhoa o sebelisang matla a centrifugal ho arola likaroloana ho latela boholo ba tsona, sebopeho, bongata le ho teteana. Ka ho etsa hore motsoako o potolohe ka lebelo le phahameng, likaroloana tse boima li qobelloa ho theohela tlase, li etsa pellet, ha likaroloana tse bobebe li ntse li leketlile ka har'a mokelikeli o matla. Katleho ea mokhoa ona e ho bokhoni ba ho arola likaroloana ka potlako tseo ho seng joalo li neng li tla hloka lihora kapa esita le matsatsi ho sebelisoa mekhoa e meng.

Mefuta ea Centrifugation

Phapang ea Centrifugation

Ho arola dipharologanyo ka dipharologanyo ke mofuta o tlwaelehileng le wa motheo wa mokhoa wa ho arola ka dipharologanyo. Mokgweng ona, sampole e otlollwa ka lebelo le tlase, e leng se lumellang karohano ya dikarolwana ho latela boholo le boima ba tsona. Ka ho eketsa lebelo butle-butle, ho ka etswa dipotoloho tse latellanang tsa ho arola ka dipharologanyo ho fihlela karohano e eketsehileng. Ts'ebetso ena e phetwa ho fihlela dikarolo tse fapaneng tsa motswako di arotswe ka ho lekaneng.

Sekhahla sa Gradient Centrifugation

Ho centrifugation ha gradient e teteaneng ke mokhoa o tsoetseng pele haholoanyane oa mokhoa oa karohano o kenyeletsang tšebeliso ea mokhoa oa gradient e teteaneng. Mokhoa ona, oo hangata e leng tharollo ea sucrose kapa cesium chloride, o etsa gradient e tšoanang ka har'a tube ea centrifuge. Motsoako o ntan'o beoa holim'a gradient, 'me ha o centrifugation, likarolo tse fapaneng li falla ka har'a tube ho latela bongata ba tsona bo phaphametseng. Mokhoa ona o nolofalletsa karohano ea likaroloana tse nang le boholo bo tšoanang empa boholo bo fapaneng.

Litšebeliso tsa Centrifugation

Baeloji ea Baeloji le ea Lisele

Ho centrifugation ho bapala karolo ea bohlokoa lefapheng la biochemistry le baeloji ea sele. Ho nolofalletsa ho aroloa le ho hloekisoa ha li-organelle tsa sele tse kang mitochondria, nuclei le ribosomes, tse bohlokoa bakeng sa ho utloisisa sebopeho le ts'ebetso ea tsona. Ka ho beha li-extracts tsa sele tlas'a centrifugation e fapaneng, bo-rasaense ba ka arola likarolo tsa sele ho latela lebelo la tsona la ho sedimentation le bongata ba tsona, ba fana ka tlhahisoleseling ea bohlokoa mabapi le mesebetsi ea tsona ea baeloji.

Tlhahlobo ea Bongaka

Ho centrifugation ho sebelisoa haholo tlhahlobong ea bongaka ho arola likarolo tsa mali. Ka ho potoloha mali ka lebelo le phahameng, lisele tse khubelu tsa mali, lisele tse tšoeu tsa mali le li-platelet li ka aroloa ho plasma. Sena se nolofalletsa tlhahlobo ea likarolo tse itseng tse kang maemo a hematocrit, lipalo tsa lisele tsa mali le liprotheine tsa plasma, ho thusa ho hlahloba mafu a fapaneng le ho beha leihlo bophelo bo botle ka kakaretso.

Indasteri ea Lijo le Lino

Ho sebelisa centrifugation ho fumana ts'ebeliso indastering ea lijo le lino, haholo-holo karohanong ea mekhahlelo e tiileng le ea metsi. Mohlala, ha ho hlahisoa lero la litholoana, centrifugation e sebelisoa ho tlosa makhasi le ho ntlafatsa ho hlaka ha sehlahisoa sa ho qetela. Ho feta moo, tlhahisong ea biri, karohano ea lisele tsa tomoso ho mokelikeli o belisitsoeng ke ea bohlokoa ho fumana sehlahisoa se hlakileng se ka nooang.

Tlhahlobo ea Tikoloho

Mekhoa ea ho centrifugation e sebelisoa litlhahlobong tse fapaneng tsa tikoloho ho fumana litšobotsi le likarolo tsa lisampole tse rarahaneng. Mohlala, lifemeng tsa kalafo ea metsi a litšila, li-centrifuge li sebelisoa ho arola seretse ho tsoa mokhahlelong oa metsi, e leng se nolofalletsang ho lahla metsi a hloekisitsoeng. Ka mokhoa o ts'oanang, tlhahlobong ea mobu, ho centrifugation ho ka thusa ho arola likaroloana tsa mobu tsa boholo bo fapaneng, ho fana ka tlhahisoleseling ea bohlokoa mabapi le monono oa mobu, sebopeho le litšila tse ka bang teng.

Melemo le Mefokolo ea Centrifugation

Melemo

Ho centrifugation ho fana ka melemo e 'maloa ho feta mekhoa e meng ea karohano. Ea pele, ke ts'ebetso e potlakileng, e lumellang bafuputsi le bo-rasaense ho fumana liphetho ka nako e khuts'oane. Ho feta moo, ho centrifugation ho sebetsa hantle haholo, ho netefatsa hore likarolo tse lakatsehang lia hlaphoheloa ka ho fetisisa. Ho feta moo, ke mokhoa o bonolo o fokotsang tšenyo ea likaroloana tse bonolo, o etsang hore e lokele lisampole tse fapaneng tsa baeloji.

Meeli

Ho sa tsotellehe melemo ea eona e mengata, ho centrifugation ho na le mefokolo e itseng. Taba ea pele, e hloka lisebelisoa tse khethehileng tse kang li-centrifuge, tse ka 'nang tsa se fumanehe habonolo lilaboratoring tsohle. Ho feta moo, mokhoa ona ha o lokele ho arola likaroloana tsa boholo bo tšoanang empa ka bongata bo tšoanang kaha li ke ke tsa aroloa hantle. Ho feta moo, ho centrifugation ka linako tse ling ho ka hlahisa khatello ea shear e ka fetolang botšepehi ba lisampole tse itseng tsa baeloji, e amang ho nepahala ha liphetho tsa ho qetela.

Qetello

Ho thiba dikhemikhale ke mokhoa o matla wa karohano o fetotseng mekgwa ya laboratori mafapheng a fapaneng a saense. Bokgoni ba yona ba ho arola ka potlako dikarolo tse itseng ho tswa metswakong e rarahaneng bo notlolletse menyetla e mengata ya ho ntshetsa pele dipatlisiso le ditshebediso tsa indasteri. Ho tloha ho biochemistry le tlhahlobo ya bongaka ho isa ho tlhahlobo ya dijo le tikoloho, ho thiba dikhemikhale ho ntse ho bapala karolo ya bohlokwa ho ntlafatseng kutlwisiso ya rona ya lefatshe la tlhaho le ho ntlafatsa maphelo a rona a letsatsi le letsatsi.