txoj kev sib cais centrifugation yog dab tsi

Txoj Kev Sib Cais Centrifugation: Kev Hloov Pauv Cov Txuj Ci Hauv Chav Kuaj Mob

Kev Taw Qhia

Hauv ntiaj teb kev tshawb fawb thiab cov txheej txheem kuaj hauv chaw kuaj mob, cov txheej txheem sib cais ua lub luag haujlwm tseem ceeb hauv kev cais cov khoom tshwj xeeb los ntawm cov khoom sib xyaw ua ke. Txawm hais tias muaj ntau txoj hauv kev muaj, centrifugation tau tshwm sim ua ib qho ntawm cov txheej txheem zoo tshaj plaws thiab siv dav tshaj plaws. Tsab xov xwm no tshawb nrhiav lub hauv paus ntsiab lus tom qab centrifugation thiab nws cov ntawv thov ntau yam hauv ntau qhov chaw tshawb fawb sib txawv.

Nkag Siab Txog Kev Siv Centrifugation

Kev siv lub zog centrifugal los cais cov khoom me me raws li lawv qhov loj me, cov duab, qhov ceev, thiab viscosity. Los ntawm kev muab cov khoom sib xyaw rau hauv kev sib hloov ceev ceev, cov khoom hnyav dua raug yuam kom mus rau hauv qab, tsim cov pellet, thaum cov khoom sib dua tseem nyob hauv cov kua supernatant. Qhov ua tau zoo ntawm cov txheej txheem no yog nyob rau hauv lub peev xwm los cais cov khoom sai sai uas yuav siv sijhawm ntau teev lossis txawm tias hnub siv lwm txoj hauv kev.

Hom Centrifugation

Kev sib txawv ntawm Centrifugation

Kev sib txawv centrifugation yog hom kev centrifugation uas siv ntau tshaj plaws thiab yooj yim tshaj plaws. Hauv txoj kev no, cov qauv raug tig ntawm qhov ceev qis, ua rau kev sib cais ntawm cov khoom me me raws li lawv qhov loj me thiab qhov hnyav. Los ntawm kev maj mam nce qhov ceev, kev sib xyob ntawm centrifugation tuaj yeem ua tiav kom ua tiav kev sib cais ntxiv. Cov txheej txheem no rov ua dua kom txog thaum ntau yam khoom sib xyaw tau sib cais txaus.

Kev Siv Centrifugation Gradient Density

Kev siv centrifugation uas muaj qhov ceev sib txawv yog ib hom kev sib cais uas siv cov khoom siv sib txawv. Cov khoom siv no, feem ntau yog cov kua sucrose lossis cesium chloride, tsim cov qauv sib xws hauv lub raj centrifuge. Tom qab ntawd cov khoom sib xyaw ua ke rau saum cov qauv sib txawv, thiab thaum centrifugation, cov khoom sib txawv txav mus raws lub raj raws li lawv cov qauv sib txawv. Cov txheej txheem no ua rau muaj kev sib cais ntawm cov khoom me me uas muaj qhov loj me zoo sib xws tab sis cov qauv sib txawv.

Cov ntawv thov ntawm Centrifugation

Biochemistry thiab Cell Biology

Kev siv centrifugation ua lub luag haujlwm tseem ceeb hauv kev tshawb fawb txog biochemistry thiab cell biology. Nws pab txhawb kev cais thiab kev ntxuav cov cellular organelles xws li mitochondria, nuclei, thiab ribosomes, uas tseem ceeb rau kev nkag siab txog lawv cov qauv thiab kev ua haujlwm. Los ntawm kev siv cov cell extracts rau kev sib txawv centrifugation, cov kws tshawb fawb tuaj yeem cais cov khoom cellular raws li lawv qhov ceev thiab qhov ceev ntawm cov dej noo, muab cov ntaub ntawv tseem ceeb txog lawv lub luag haujlwm biological.

Kev Kuaj Mob Hauv Tsev Kho Mob

Kev siv lub tshuab centrifugation dav dav hauv kev kuaj mob kom cais cov khoom ntshav. Los ntawm kev tig cov ntshav ntawm qhov ceev ceev, cov qe ntshav liab, cov qe ntshav dawb, thiab cov platelets tuaj yeem cais tawm ntawm cov plasma. Qhov no ua rau muaj kev tshuaj xyuas cov khoom tshwj xeeb xws li cov theem hematocrit, cov lej qe ntshav, thiab cov protein plasma, pab hauv kev kuaj mob ntawm ntau yam kab mob thiab kev saib xyuas kev noj qab haus huv tag nrho.

Kev Lag Luam Khoom Noj thiab Dej Haus

Kev siv centrifugation pom muaj kev siv hauv kev lag luam khoom noj thiab dej haus, tshwj xeeb tshaj yog hauv kev sib cais cov khoom khov thiab cov kua. Piv txwv li, thaum tsim cov kua txiv hmab txiv ntoo, centrifugation siv los tshem tawm cov pulp thiab txhim kho qhov meej ntawm cov khoom kawg. Tsis tas li ntawd, hauv kev tsim npias, kev sib cais cov qe poov xab los ntawm cov kua fermented yog qhov tseem ceeb kom tau txais cov khoom ntshiab haus tau.

Kev Tshawb Fawb Txog Ib Puag Ncig

Cov txheej txheem centrifugation siv rau hauv ntau yam kev tshuaj xyuas ib puag ncig los txiav txim siab cov khoom thiab cov khoom sib xyaw ntawm cov qauv nyuaj. Piv txwv li, hauv cov chaw kho dej khib nyiab, centrifuges siv los cais cov sludge ntawm cov kua dej, ua kom yooj yim rau kev pov tseg cov dej kho. Ib yam li ntawd, hauv kev tshuaj xyuas av, centrifugation tuaj yeem pab cais cov av me me ntawm ntau qhov ntau thiab tsawg, muab cov ntaub ntawv tseem ceeb txog kev nplua nuj ntawm av, cov khoom sib xyaw, thiab cov kuab paug.

Cov Zoo thiab Cov Kev Txwv ntawm Centrifugation

Cov txiaj ntsig

Kev siv centrifugation muaj ntau qhov zoo dua li lwm cov txheej txheem sib cais. Ua ntej, nws yog ib qho txheej txheem ceev, uas tso cai rau cov kws tshawb fawb thiab cov kws tshawb fawb kom tau txais cov txiaj ntsig hauv lub sijhawm luv luv. Tsis tas li ntawd, centrifugation ua haujlwm tau zoo heev, ua kom ntseeg tau tias cov khoom seem rov qab tau zoo tshaj plaws. Ntxiv mus, nws yog ib qho txheej txheem mos mos uas txo qhov kev puas tsuaj rau cov khoom me me, ua rau nws haum rau ntau yam qauv tshuaj lom neeg.

Cov kev txwv

Txawm hais tias nws muaj ntau yam zoo, centrifugation muaj qee qhov kev txwv. Ua ntej, nws xav tau cov khoom siv tshwj xeeb xws li centrifuges, uas tej zaum yuav tsis muaj nyob rau hauv txhua lub chaw soj nstuam. Tsis tas li ntawd, cov txheej txheem no tsis haum rau kev sib cais cov khoom me me uas muaj tib qhov loj me tab sis muaj cov ntom zoo sib xws vim tias lawv yuav tsis sib cais kom zoo. Ntxiv mus, centrifugation qee zaum tuaj yeem ua rau muaj kev ntxhov siab uas yuav hloov pauv qhov kev ncaj ncees ntawm qee cov qauv tshuaj lom neeg, cuam tshuam rau qhov tseeb ntawm cov txiaj ntsig kawg.

Xaus lus

Kev siv centrifugation yog ib txoj kev sib cais muaj zog uas tau hloov pauv txoj kev kuaj mob hauv ntau qhov chaw tshawb fawb. Nws lub peev xwm los cais cov khoom tshwj xeeb sai sai los ntawm cov khoom sib xyaw ua ke tau qhib ntau lub sijhawm rau kev txhim kho kev tshawb fawb thiab kev siv hauv kev lag luam. Txij li biochemistry thiab kev kuaj mob mus rau kev tshuaj xyuas zaub mov thiab ib puag ncig, kev siv centrifugation tseem ua lub luag haujlwm tseem ceeb hauv kev txhim kho peb txoj kev nkag siab txog lub ntiaj teb ntuj thiab txhim kho peb lub neej txhua hnub.