Centrifugation e arola eng

Centrifugation: E arola eng?

Selelekela sa Centrifugation

Ho centrifugation ke mokhoa o matla o sebelisoang haholo mafapheng a fapaneng a saense, ho kenyeletsoa baeloji, k'hemistri le bongaka. Joalokaha lebitso la oona le bontša, ho centrifugation ho kenyelletsa ho potoloha ha disampole ka lebelo le phahameng. Ho potoloha hona ho hlahisa matla a centrifugal a ka arolang likarolo tse fapaneng tsa motsoako ho latela bongata ba tsona kapa boholo ba tsona. Ka ho sebelisa melao-motheo ea ho centrifugation, bo-rasaense ba ka arola le ho hloekisa lintho tse reriloeng ka katleho ho tsoa metsoakong e rarahaneng. Sehloohong sena, re tla hlahloba metheo ea ho centrifugation le ho buisana ka lintho tse fapaneng tseo e ka li arolang.

Ho Arola Likarolo tsa Mali: Matla a ho Centrifugation

E 'ngoe ea lits'ebetso tse tloaelehileng tsa ho centrifugation ke karohanong ea likarolo tsa mali. Mali a entsoe ka lisele tse khubelu tsa mali (erythrocytes), lisele tse tšoeu tsa mali (leukocytes), li-platelet le plasma. Ho fumana likarolo tse itseng bakeng sa merero ea tlhahlobo kapa ea kalafo, centrifugation e phetha karolo ea bohlokoa. Ka ho potoloha mali ka centrifuge e khethehileng, lisele tse khubelu tsa mali tse teteaneng li pelletsoa ka tlase, li etsa lera le tsejoang e le hematocrit. Ka holim'a lera la hematocrit, ho hlaha lera le boreleli, le nang le lisele tse tšoeu tsa mali le li-platelet. Qetellong, plasma, karolo e metsi ea mali, e lula lera le ka holimo. Karohano ena e lumella ho aroloa ha likarolo tse fapaneng tsa mali bakeng sa tlhahlobo kapa ts'ebetso e tsoelang pele.

Ho Fokotsa Dikarolo tsa Disele: Ho Arola Di-organelle tsa Disele

Li-organelle tsa sele ke likarolo tsa bohlokoa ka har'a lisele tse etsang mesebetsi e itseng. Hangata bo-rasaense ba hloka ho arola le ho ithuta li-organelle tsena ho utloisisa haholoanyane lits'ebetso tsa sele. Ho arola ka mokhoa o fapaneng ke mokhoa o sebelisang bongata bo fapaneng ba li-organelle ho li arola. Ka ho beha li-homogenate tsa sele kapa ho ntša lintho tse ling letotong la mehato ea ho arola ka lebelo le fapaneng, li-organelle li ka aroloa ka likaroloana tse fapaneng. Mohlala, ho arola ka mokhoa o tlase ka lebelo le tlase ho tlosa meaho e meholohali ea sele, joalo ka li-nuclei le litšila. Ho matha ka lebelo le phahameng ho ka baka pellet mitochondria, endoplasmic reticulum, kapa lysosomes ka likaroloana tse arohaneng. Mokhoa ona o lumella bafuputsi ho ithuta li-organelle ka bomong le mesebetsi ea tsona.

Ho arola DNA ka Centrifugation: Mokhoa oa Phetohelo

Lefapheng la baeloji ea limolek'hule, ho aroloa ha DNA ke mohato oa bohlokoa litekong kapa litlhahlobong tse fapaneng. Ho aroloa ha DNA ho fetotse mokhoa oa ho ntšoa ha DNA, ho etsa hore e sebetse hantle le ho boloka nako. Mokhoeng ona, motsoako o nang le DNA, liprotheine le likarolo tse ling tsa sele o kenngoa ka har'a centrifugation. Kaha DNA ke molek'hule e telele, e kang khoele, e tsoa ka har'a tharollo habonolo ho feta lintho tse ling ha matla a centrifugal a sebelisoa. Ka ho laola maemo a centrifugal ka hloko, bo-rasaense ba ka fumana DNA e hloekisitsoeng e ka sebelisoang bakeng sa lithuto tsa liphatsa tsa lefutso, tatellano, kapa mekhoa e meng ea limolek'hule.

Ho Arola Likarolo tsa Sele: Karolo ea Density Gradient Centrifugation

Ho sebelisa centrifugation ho sebelisa density gradient media ho lumella karohano ea likarolo tse nang le density gradient tse tšoanang empa boholo bo fapane. Ka ho theha seaplane se nang le density gradient (e phahameng tlase le e tlase holimo), bo-rasaense ba ka bona ho falla ha likarolo tse fapaneng ha li fihla maemong a tsona a tekano ho latela density. Mohlala, ha li sebelisoa motswakong oa liprotheine ka density gradient, liprotheine tse boima li tla teba ho ea tlase, athe tse bobebe li tla phaphamala ho ea holimo. Mokhoa ona o thusa haholo bakeng sa ho arola lipoprotein lithutong tsa cholesterol kapa ho arola livaerase.

Qetello:

Ho centrifugation ke mokhoa o feto-fetohang o nang le lits'ebetso tse ngata lipatlisisong tsa saense le tlhahlobo ea bongaka. E fetohile karolo ea bohlokoa ea mekhoa ea laboratori, e lumellang bo-rasaense ho arola lintho tse fapaneng ho latela bongata ba tsona kapa boholo ba tsona. Ho tloha ho arola likarolo tsa mali ho isa ho arola li-organelle tsa sele le DNA, ho centrifugation ho ntse ho bapala karolo ea bohlokoa ho ntlafatseng kutloisiso ea saense le ho ntlafatsa mekhoa ea tlhokomelo ea bophelo. Ka ho sebelisa matla a matla a centrifugal, bafuputsi ba ka arola le ho hloekisa likarolo tse reretsoeng ka katleho, ba bula tsela bakeng sa lintho tse ncha tse sibolotsoeng le tsoelo-pele mafapheng a fapaneng.