dab tsi ua rau centrifugation cais
Centrifugation: Nws Sib Cais Li Cas?
Kev Taw Qhia Txog Centrifugation
Kev centrifugation yog ib txoj kev siv zog uas siv dav hauv ntau qhov chaw tshawb fawb, suav nrog biology, chemistry, thiab tshuaj. Raws li nws lub npe qhia, centrifugation cuam tshuam nrog kev tig cov qauv ntawm qhov ceev ceev. Qhov kev tig no tsim lub zog centrifugal uas tuaj yeem cais cov khoom sib txawv ntawm cov khoom sib xyaw raws li lawv qhov ceev lossis qhov loj me. Los ntawm kev siv cov ntsiab cai ntawm centrifugation, cov kws tshawb fawb tuaj yeem cais thiab ntxuav cov khoom lag luam los ntawm cov khoom sib xyaw ua ke. Hauv tsab xov xwm no, peb yuav tshawb nrhiav cov hauv paus ntawm centrifugation thiab tham txog ntau yam khoom uas nws tuaj yeem cais.
Kev Cais Cov Cheebtsam Ntshav: Lub Hwj Chim ntawm Centrifugation
Ib qho ntawm cov kev siv centrifugation feem ntau yog kev sib cais cov khoom ntshav. Cov ntshav muaj cov qe ntshav liab (erythrocytes), cov qe ntshav dawb (leukocytes), platelets, thiab plasma. Yuav kom tau txais cov khoom tshwj xeeb rau kev kuaj mob lossis kev kho mob, centrifugation ua lub luag haujlwm tseem ceeb. Los ntawm kev tig cov ntshav hauv lub centrifuge tshwj xeeb, cov qe ntshav liab ntom dua raug pelleted ntawm qab, tsim ib txheej hu ua hematocrit. Saum toj ntawm txheej hematocrit, lub tsho buffy tshwm sim, muaj cov qe ntshav dawb thiab platelets. Thaum kawg, plasma, cov khoom kua ntawm cov ntshav, nyob rau sab saum toj txheej. Qhov kev sib cais no tso cai rau kev sib cais ntawm cov khoom ntshav sib txawv rau kev tshuaj xyuas lossis kev ua ntxiv.
Kev Sib Txawv Centrifugation: Kev Sib Cais Cov Organelles Cellular
Cov organelles hauv lub cev yog cov khoom tseem ceeb hauv cov cell uas ua haujlwm tshwj xeeb. Cov kws tshawb fawb feem ntau yuav tsum cais tawm thiab kawm cov organelles no kom nkag siab zoo dua txog cov txheej txheem cellular. Kev sib txawv centrifugation yog ib txoj kev uas siv qhov sib txawv ntawm cov organelles los cais lawv. Los ntawm kev muab cov cell homogenates lossis extracts rau ntau cov kauj ruam centrifugation ntawm qhov ceev sib txawv, organelles tuaj yeem raug cais tawm ua cov feem sib txawv. Piv txwv li, thawj centrifugation ntawm qhov ceev qis tshem tawm cov qauv cellular loj tshaj plaws, xws li nuclei thiab cov khib nyiab. Tom qab ntawd khiav ntawm qhov ceev siab dua tuaj yeem pellet mitochondria, endoplasmic reticulum, lossis lysosomes hauv cov feem sib cais. Cov txheej txheem no tso cai rau cov kws tshawb fawb kawm txog cov organelles thiab lawv cov haujlwm.
Kev rho tawm DNA los ntawm Centrifugation: Ib Txoj Kev Hloov Pauv Tshiab
Hauv kev kawm txog molecular biology, kev rho tawm DNA yog ib kauj ruam tseem ceeb hauv ntau yam kev sim lossis kev tshuaj xyuas. Centrifugation tau hloov pauv txoj kev rho tawm DNA, ua rau nws ua haujlwm tau zoo dua thiab siv sijhawm ntev dua. Hauv cov txheej txheem no, ib qho sib xyaw uas muaj DNA, cov protein, thiab lwm yam khoom siv hauv lub cev raug centrifugation. Vim tias DNA yog ib qho molecule ntev, zoo li xov, nws precipitates tawm ntawm cov kua yooj yim dua li lwm yam khoom thaum lub zog centrifugal siv. Los ntawm kev ua tib zoo tswj cov xwm txheej centrifugal, cov kws tshawb fawb tuaj yeem tau txais DNA huv uas tuaj yeem siv rau kev tshawb fawb txog genetic, sequencing, lossis lwm yam txheej txheem molecular.
Kev Cais Cov Cheebtsam Cellular: Lub Luag Haujlwm ntawm Density Gradient Centrifugation
Kev siv cov khoom siv centrifugation siv cov khoom siv density gradient tso cai rau kev sib cais ntawm cov khoom uas muaj qhov sib xws tab sis qhov loj sib txawv. Los ntawm kev tsim cov khoom siv nrog qhov sib txawv ntawm qhov sib txawv (siab dua ntawm hauv qab thiab qis dua ntawm sab saum toj), cov kws tshawb fawb tuaj yeem pom kev tsiv teb tsaws chaw ntawm cov khoom sib txawv thaum lawv mus txog lawv qhov chaw sib npaug raws li qhov sib txawv. Piv txwv li, thaum siv rau cov protein sib xyaw hauv qhov sib txawv ntawm qhov sib txawv, cov protein hnyav dua yuav poob rau hauv qab, thaum cov sib dua yuav ntab mus rau sab saum toj. Cov txheej txheem no yog qhov tshwj xeeb tshaj yog rau kev sib cais lipoproteins hauv kev tshawb fawb txog cholesterol lossis kev cais cov kab mob.
Xaus lus:
Kev siv centrifugation yog ib txoj kev siv tau ntau yam hauv kev tshawb fawb thiab kev kuaj mob. Nws tau dhau los ua ib feem tseem ceeb ntawm cov txheej txheem kuaj mob, uas tso cai rau cov kws tshawb fawb cais ntau yam khoom raws li lawv qhov ceev lossis qhov loj me. Txij li kev cais cov khoom ntshav mus rau kev cais cov organelles cellular thiab DNA, centrifugation tseem ua lub luag haujlwm tseem ceeb hauv kev txhim kho kev nkag siab txog kev tshawb fawb thiab txhim kho kev kho mob. Los ntawm kev siv lub zog ntawm centrifugal force, cov kws tshawb fawb tuaj yeem cais thiab ntxuav cov khoom tseem ceeb, ua rau txoj hauv kev rau kev tshawb pom tshiab thiab kev nce qib hauv ntau qhov chaw.
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