Ho arola mali ka sentrifugation ho arola mali joang

E 'ngoe ea mekhoa ea motheo lefapheng la lipatlisiso tsa bongaka le tlhahlobo ea bongaka ke ho centrifugation. Ts'ebetso ena e kenyelletsa ho potoloha lisampole ka lebelo le phahameng ho arola likarolo tse fapaneng ho latela bongata ba tsona bo fapaneng. Ho centrifugation ho bohlokoa haholo ha ho tluoa tabeng ea ho arola likarolo tse itseng maling. Sehloohong sena, re tla hlahloba kamoo mekhoa ea ho centrifugation e sebelisoang ho arola mali ka likarolo tsa eona tse fapaneng, joalo ka plasma, li-platelet le lisele tse khubelu le tse tšoeu tsa mali. Re tla hlahloba melao-motheo e ka morao ho centrifugation, mefuta ea centrifuge e sebelisoang, le ts'ebeliso ea mokhoa ona maemong a bongaka le lipatlisiso.

I. Selelekela

Mali ke karolo ea bohlokoa ea 'mele oa motho e etsang mesebetsi ea bohlokoa joalo ka ho tsamaisa oksijene, karabelo ea 'mele ea ho itšireletsa mafung le ho tlosa litšila. A na le likarolo tse 'maloa, ho kenyeletsoa plasma, lisele tse khubelu tsa mali (RBC), lisele tse tšoeu tsa mali (WBC), le li-platelet. Bakeng sa merero e fapaneng ea tlhahlobo le lipatlisiso, ho hlokahala ho arola likarolo tsena ho li ithuta ka bonngoe, ho sekaseka thepa ea tsona, kapa ho li sebelisa bakeng sa mekhoa e itseng ea bongaka.

II. Melao-motheo ea ho Centrifugation

Ho centrifugation ho sebedisa melaomotheo ya ho sedimentation le density gradient ho arola dikarolo tse fapaneng tsa madi. Ha sampole e phetholwa ka lebelo le phahameng ka hara centrifuge, dikarolwana tse teteaneng di ya ka thoko, ha dikarolwana tse se nang boima bo bokane bohareng ba sampole. Tshusumetso ya matla a centrifugal e nolofalletsa karohano ya dikarolo tsa madi ho latela bongata ba tsona, ka dikarolwana tse boima di fallela tlase kapa di etsa pellet.

III. Mefuta ea Li-Centrifuge

Ho na le mefuta e fapaneng ea li-centrifuge tse sebelisetsoang karohano ea mali. Tsena li kenyelletsa li-centrifuge tsa tabletop, li-microcentrifuge, li-ultracentrifuge, le li-centrifuge tse sehatsetsing. Mofuta o mong le o mong o na le litšobotsi tsa oona, lebelo le bokhoni ba oona. Li-centrifuge tsa tabletop li sebelisoa hangata lilaboratoring tsa bongaka kaha li fana ka tekano pakeng tsa bokhoni ba lebelo le phahameng le bokhoni ba bophahamo ba sampole.

IV. Ho lokisa disampole tsa Mali

Pele ho centrifugation, ho bohlokoa ho lokisa disampole tsa mali ka nepo ho netefatsa karohano e nepahetseng ea likarolo. Mali hangata a bokelloa ho sebelisoa li-anticoagulant ho thibela ho hoama ha mali. Sampole e ntan'o tsoakoa ka hloko ebe e fetisetsoa liphaepheng tse loketseng tsa centrifuge, ho hlokomeloa hore ha e kenye lipululana tsa moea. Liphaephe tsa centrifuge li koaletsoe ka mokhoa o sireletsehileng ho thibela ho lutla 'me li beoa ka har'a rotor ea centrifuge ka litlhophiso tse leka-lekaneng.

V. Karohano ea Li-Plasma le Likarolo tsa Lisele

Nakong ea ho centrifugation, likarolo tsa mali lia arohana ka mekhahlelo e fapaneng ka har'a lipeipi tsa centrifuge. Plasma, karolo ea mali e bosootho bo bosehla, e nyolohela holimo kaha ke karolo e teteaneng haholo. Lera lena le ntšoa ka hloko ho sebelisoa pipette kapa lisebelisoa tse itseng tsa karohano ea plasma. Likarolo tse setseng tsa sele, ho kenyeletsoa le RBC, WBC, le liplatelete, li etsa pellet tlase ho tube.

VI. Ho Aroloa ha Lisele tse Khubelu le tse Tšoeu tsa Mali

Kamora ho arola plasma, pellet e nang le RBC, WBC, le liplatelete e tlameha ho sebetsoa ka ho eketsehileng ho arola likarolo ka bonngoe. Pellet e kenngoa hape ka bonolo tharollong e loketseng e bitsoang tharollo ea ho hlatsoa. ​​Mohato ona o kenyelletsa ho eketsa buffer le ho kopanya butle ho emisa pellet ka ho lekana. Ebe disampole li kenngoa ka potoloho e latelang ea centrifugation ho arola RBC le WBC.

VII. Ho Sebelisa Centrifugation ho Arola Li-Platelet

Li-platelet, tsa bohlokoa bakeng sa ho hoama le ho folisa maqeba, li ka fumanoa ka mokhoa o bitsoang tokisetso ea plasma e ruileng ka li-platelet (PRP). Ka ho sebelisa mekhoa e itseng ea centrifugation, PRP e ka aroloa le ho bokelloa ho tsoa lera la plasma. PRP e sebelisoa haholo lits'ebetsong tse fapaneng tsa bongaka, ho kenyeletsoa kalafo ea masapo, pholiso ea maqeba le dermatology.

VIII. Ts'ebeliso ea Centrifugation Libakeng tsa Bongaka le Lipatlisiso

Ho centrifugation ho bapala karolo ea bohlokoa lilaboratoring tsa bongaka le tsa lipatlisiso. Ntle le karohano ea likarolo tsa mali, e sebelisoa haholo ho aroleng li-organelle tse ka tlas'a sele, ho hloekisa liprotheine, le ho ithuta likamano tsa sele. Ho centrifugation ho boetse ho fumana lits'ebetso ho hloekiseng livaerase le likaroloana tse nyane, liteko tsa sele, le lipatlisiso tsa meriana.

Qetellong, ho centrifugation ke mokhoa oa bohlokoa oa ho arola likarolo tsa mali le ho li ithuta ka bonngoe. Ka ho sebelisa melao-motheo ea ho sedimentation le density gradient, likarolo tse fapaneng tse kang plasma, RBC, WBC, le platelet li ka aroloa ka katleho. Ho hlaha ha mefuta e fapaneng ea centrifuge ho kentse letsoho haholo tsoelo-peleng ea tlhahlobo ea bongaka le lipatlisiso. Ka mefuta e mengata ea ts'ebeliso, centrifugation e ntse e tsoela pele ho nolofatsa litšibollo tse ncha lefapheng la mahlale a bongaka.