Kuparadzanisa ropa ne "centrifugation" kunoparadzanisa sei ropa

Imwe yenzira dzakakosha mumunda wekutsvaga kwekurapa uye kuongororwa kwekiriniki ndeye centrifugation. Maitiro aya anosanganisira kutenderera masampuli nekumhanya kwakanyanya kuparadzanisa zvikamu zvakasiyana zvichienderana nekuwanda kwazvo. Centrifugation inonyanya kukosha kana zvasvika pakuparadzanisa zvikamu zvakati kubva muropa. Muchinyorwa chino, tichaongorora kuti nzira dze centrifugation dzinoshandiswa sei kuparadzanisa ropa muzvikamu zvakasiyana-siyana, zvakaita seplasma, maplatelet, uye masero matsvuku nemachena eropa. Tichaongorora misimboti iri shure kwe centrifugation, mhando dze centrifuges dzinoshandiswa, uye mashandisirwo enzira iyi munzvimbo dzekurapa nedzekutsvagisa.

I. Nhanganyaya

Ropa chinhu chakakosha mumuviri wemunhu chinoita mabasa akakosha akadai sekutakura okisijeni, kupindura kwemuviri kumuviri, uye kubvisa tsvina. Rine zvikamu zvakati wandei, zvinosanganisira plasma, masero matsvuku eropa (RBCs), masero machena eropa (WBCs), uye maplatelet. Pazvinangwa zvakasiyana-siyana zvekuongorora nekutsvaga, zvinova zvakakosha kupatsanura zvikamu izvi kuti uzvidzidze wega wega, kuongorora hunhu hwazvo, kana kuzvishandisa pakurapa kwakananga.

II. Nheyo dzeCentrifugation

Kubatanidza ropa kunoshandisa nzira yekuisa ropa muropa uye kupatsanura huwandu hweropa kuti riparadzanise zvikamu zvakasiyana zveropa. Kana sampuli ikatenderedzwa nekumhanya kwakanyanya mu centrifuge, zvikamu zvakakora zvinofamba zvichienda kumucheto, nepo zvikamu zvisina kukora zvinoungana zvichienda pakati pemuenzaniso. Simba re centrifugal rinoita kuti zvikamu zveropa zviparadzaniswe zvichienderana nekuwanda kwazvo, zvikamu zvinorema zvichienda pasi kana kugadzira pellet.

III. Mhando dzeCentrifuges

Kune mhando dzakasiyana dzema "centrifuge" anoshandiswa pakupatsanura ropa. Izvi zvinosanganisira "tabletop centrifuges", "microcentrifuges", "ultracentrifuges", uye "refrigerated centrifuges". Rudzi rumwe nerumwe rune maficha arwo, kumhanya, uye kugona. "tabletop centrifuges" dzinowanzo shandiswa mumakiriniki sezvo dzichipa chiyero pakati pesimba rekumhanya uye simba rehuwandu hwesampuro.

IV. Kugadzirira sampuro dzeRopa

Usati waisa centrifugation, zvakakosha kugadzirira masampuli eropa nemazvo kuti uve nechokwadi chekupatsanurwa kwakarurama kwezvikamu zvacho. Ropa rinowanzounganidzwa uchishandisa mishonga inorwisa ropa kudzivirira kugwinha kweropa. Muenzaniso wacho unozosanganiswa nokungwarira woendeswa kumachubhu akakodzera ecentrifuge, uchingwarira kuti usapinze mabubble emhepo. Machubhu ecentrifuge anovharwa zvakachengeteka kudzivirira kubuda kweropa uye anoiswa mu centrifuge rotor nenzira yakaringana.

V. Kupatsanurwa kwePlasma neCellular Components

Munguva ye centrifugation, zvikamu zveropa zvinopatsanuka kuita zvikamu zvakasiyana mukati memachubhu e centrifuge. Plasma, chikamu cheropa chine mvura yero, chinokwira kusvika kumusoro sezvo chiri chikamu chisina kusimba zvakanyanya. Chikamu ichi chinobviswa nokungwarira uchishandisa pipette kana michina chaiyo yekuparadzanisa plasma. Zvimwe zvikamu zvemasero zvasara, zvinosanganisira maRBC, maWBC, uye maplatelet, zvinoumba pellet pasi pechubhu.

VI. Kuparadzaniswa kweMasero Matsvuku neMachena eRopa

Mushure mekupatsanura plasma, pellet ine maRBC, maWBC, uye maplatelet inofanira kugadziriswa zvakanyanya kuti zvikamu zvacho zviparadzaniswe. Pellet inosungirirwa zvinyoro nyoro mumushonga wakakodzera unonzi washing solution. Danho iri rinosanganisira kuwedzera buffer uye kusanganisa zvinyoro nyoro kuti pellet igare yakabatana. Sampuli dzinozoiswa muchikamu chinotevera che centrifugation kuti dziparadzanise maRBC nemaWBC.

VII. Kushandisa Centrifugation Kubvisa Maplatelet

Maplatelet, akakosha pakugwamba kweropa uye kuporesa maronda, anogona kuwanikwa nemaitiro anonzi platelet-rich plasma (PRP). Nekushandisa nzira dzakatarwa dze centrifugation, PRP inogona kuparadzaniswa uye kuunganidzwa kubva muplasma layer. PRP inoshandiswa zvakanyanya mukurapa kwakasiyana-siyana, kusanganisira kurapwa kwemapfupa, kuporesa maronda, uye dermatology.

VIII. Mashandisirwo eCentrifugation muZvirongwa zvekurapa nekutsvaga

Kuchenesa ropa (centrifugation) kunoita basa rakakosha mumarabhoritari ekurapa neokutsvaga ruzivo. Pamusoro pekuparadzanisa zvikamu zveropa, kunoshandiswa zvakanyanya mukuparadzanisa maorganelle ari pasi pemasero, kuchenesa mapuroteni, uye kudzidza kudyidzana kwemasero. Kuchenesa ropa (centrifugation) kunobatsirawo mukuchenesa mavhairasi netunhu tudiki, kuyedza masero, uye kutsvakurudza kwemishonga.

Mukupedzisa, centrifugation inzira inokosha yekuparadzanisa zvikamu zveropa uye kuzvidzidza chimwe nechimwe. Nekushandisa misimboti yekudonha kweropa uye density gradient, zvikamu zvakasiyana zvakaita seplasma, RBCs, WBCs, uye platelets zvinogona kupatsanurwa zvinobudirira. Kuuya kwemhando dzakasiyana dze centrifuges kwakabatsira zvikuru mukufambira mberi mukuongorora nekutsvaga mishonga. Nekushandiswa kwayo kwakasiyana-siyana, centrifugation inoramba ichibatsira kuwana zvinhu zvitsva mumunda wesainzi yezvekurapa.