Centrifugation inoparadzanisa sei organelles dzemasero

Centrifugation Inoparadzanisa Sei Organelles dzeSero?

Nhanganyaya:

Kupinza masero mu centrifugation inzira yakakosha inoshandiswa mukutsvagisa kwezvipenyu kupatsanura ma organelle emasero zvichienderana nehukuru hwawo, huwandu hwawo, uye mwero wekudonha kwemvura. Nekuisa sampuro kumasimba ekutenderera anomhanya, kupinza masero mu centrifugation kunoshandisa mutsauko uri muhunhu uhwu, zvichibvumira masayendisiti kupatsanura ma organelle chaiwo kuti awedzere kuongororwa. Chinyorwa chino chinotsanangura misimboti iri shure kwekuisa masero mu biology yemasero.

1. Nheyo dzeCentrifugation:

Kupinza zvinhu mu centrifuge kunosanganisira kushandisa centrifuge, muchina unotenderedza masampuli nekumhanya kukuru kuti ugadzire masimba e centrifugal. Masimba aya anokonzera kuti zvidimbu zviende mumatope, zvidimbu zvinorema uye zvikuru zvigadzikane nekukurumidza kupfuura zvakareruka nezvidiki. Kupatsanurwa uku kunowanikwa nekukanda zvidimbu zvinorema pasi pechubhu, nokudaro zvichizviparadzanisa nezvimwe zvese zviri musampuli.

2. Kusiyanisa kweCentrifugation:

Kuparadzanisa ma organelles emasero nenzira ye differential ndiyo nzira inonyanya kushandiswa kupatsanura ma organelles emasero. Inosanganisira matanho akatevedzana e centrifugation, rimwe nerimwe richiwedzera simba re centrifugal uye nguva yaro. Chinangwa ndechekuparadzanisa ma organelles zvishoma nezvishoma zvichienderana nehukuru hwawo uye huwandu hwawo.

Munguva yekutenderera kwekutanga kusingamhanye zvakanyanya, marara makuru nemanuclei zvinogara pasi, zvichiita pellet. Supernatant, ine maorganelle madiki akadai semitochondria nelysosomes, inounganidzwa uye inoiswa kune imwe denderedzwa ye centrifugation nekumhanya kwakanyanya. Maitiro aya anodzokororwa kakawanda, zvichikonzera kuparadzaniswa kwe maorganelle chaiwo.

3. Kudzora Kuwanda Kwemagiraidhi:

Kuyera density gradient centrifugation inzira yakanyatsonaka inoparadzanisa organelles kwete nehukuru chete asiwo nehuremu. Munzira iyi, zvikamu zviviri kana kupfuura zvemvura zvine huremu huri kuwedzera zvinoiswa muchubhu ye centrifuge. Kana zvikaitwa centrifugation, organelles dzinofamba nepakati pe density gradient kusvika dzasvika kunzvimbo iyo huremu hwadzo hunoenderana nehwenzvimbo yakapoteredza.

Ma organelles anoumba mabhandi akasiyana kana nzvimbo dziri mukati me gradient, zvichibvumira kuunganidzwa kwawo uye kuongororwa kwakawedzerwa. Iyi nzira inonyanya kubatsira pakuparadzanisa ma organelles akapfava, akadai se peroxisomes ne Golgi apparatus, ane saizi dzakafanana asi densities dzakasiyana.

4. Kudzora kupisa kwemagetsi zvakanyanya:

Kupinza simba remagetsi (Ultracentrifuge) kunosanganisira kushandisa ma "high-speed centrifuge" anogona kugadzira masimba anodarika simba repasi rinokweva rakapetwa ka100,000. Nzira iyi inoshandiswa pakuparadzanisa ma "organelles" madiki uye akakora, akadai se "microsomes", "ribosomes", uye "virusi".

Nekuisa sampuro pasi pesimba rakadaro, ultracentrifugation inogona kuparadzanisa zvidimbu kusvika padanho repamusoro. Maitiro e sedimentation anowanikwa anoshandiswa kupatsanura zvikamu zvemasero zvichienderana nehukuru hwazvo uye huwandu hwazvo.

5. Mashandisirwo muCell Biology:

Kugadzira centrifugation chishandiso chakakosha mu biology yemasero chine mashandisirwo akasiyana-siyana. Nekuparadzanisa nekuchenesa ma organelles chaiwo, vaongorori vanogona kudzidza mashandiro awo, kuumbwa kwemapuroteni, uye kudyidzana mukati memasero.

Chimwe chinhu chakakosha ndechekunzwisisa mashandiro emitochondrial uye kusashanda zvakanaka kwemasero. Kudzora masero kunobvumira vaongorori kuparadzanisa mitochondria kubva munyama dzakasiyana kana masero uye kuongorora bioenergetics yavo, metabolism, uye basa ravo muzvirwere zvakaita seParkinson's kana Alzheimer's.

Imwe nzira iri mukudzidza nezvemavhairasi nehutachiona. Kushandiswa kweUltracentrifugation kunogonesa kucheneswa kwezvikamu zvehutachiona kubva mumasero ane hutachiona, zvichibatsira mukuona chimiro chazvo, majini, uye mashandiro ekuberekana kwehutachiona.

Uyezve, centrifugation inoita basa guru mumunda weproteomics nekuparadzanisa masero anobatanidzwa mukugadzirwa kweprotein uye kutengeswa kwayo. Izvi zvinobatsira ongororo yenzvimbo yemapuroteni, kugadziriswa kwemashure ekushandurwa, uye kudyidzana kwemapuroteni nemapuroteni.

Mhedziso:

Kubatanidza masero (centrifugation) inzira yakakosha yekuparadzanisa ma cell organelles zvichienderana nehukuru hwawo, density, uye sedimentation rates. Kuburikidza nekushandisa differential centrifugation uye density gradient centrifugation, ma specific organelles anogona kupatsanurwa uye kudzidzwa zvakadzama. Mhando dzakasiyana dzemashandisirwo eiyi nzira mu cell biology dzinobatsira zvikuru mukunzwisisa kwedu maitiro emasero uye kukura kwemishonga.