Pehea e hoʻokaʻawale ai ka centrifuge i nā hui

Nā Centrifuges: Ke wehe nei i nā mea huna o ka hoʻokaʻawale ʻana i ka hui ʻana

Hoʻolauna:

He mau mea hana ikaika nā Centrifuges i hoʻohana ʻia i nā noi ʻepekema a me nā ʻoihana like ʻole e hoʻokaʻawale i nā hui paʻakikī i loko o kā lākou mau ʻāpana pākahi. Hoʻohana kēia mau mea hana kupaianaha i nā kumumanaʻo o ka ikaika centrifugal e hoʻokō i ka hoʻokaʻawale pololei, e hāʻawi ana i nā mea noiʻi a me nā loea i nā ʻike waiwai nui i loko o nā ʻano mea like ʻole. Ma kēia ʻatikala, e ʻimi mākou pehea e hoʻokaʻawale ai nā centrifuges i nā hui, kā lākou noi ma nā ʻano like ʻole, a me nā ʻano hana e hoʻolilo ai iā lākou i mea maikaʻi.

I. Ke Hoʻomaopopo ʻana i ka Mana Centrifugal:

I mea e hoʻomaopopo ai i ke ʻano o ka hana ʻana o nā centrifuges, pono mua kākou e ʻeli hohonu i loko o ke kumumanaʻo o ka ikaika centrifugal. Ke wili kahi mea a puni kahi axis waena, e like me ka wili ʻana a i ʻole ka merry-go-round, loaʻa iā ia kahi ikaika huki i waho i ʻike ʻia ʻo ka ikaika centrifugal. Hoʻoikaika kēia ikaika e hoʻokuke i nā mea mai ke kikowaena o ka wili ʻana.

II. Ka Hoʻohana ʻana i ka Mana Centrifugal no ka Hoʻokaʻawale ʻana:

Hoʻohana nā centrifuges i kēia ikaika centrifugal e hoʻokaʻawale i nā hui ʻana ma muli o nā ʻano mānoanoa like ʻole o kā lākou mau ʻāpana. Ma ka milo ʻana i nā wikiwiki kiʻekiʻe, hana ka centrifuge i kahi kahua centrifugal e hoʻopuka ai i kahi ikaika ikaika i waho. ʻO kēia ikaika ke kumu o nā ʻāpana a i ʻole nā ​​​​mea i loko o ka hui ʻana e neʻe i nā ʻaoʻao o waho, ʻoiai nā ʻāpana māmā e noho kokoke ana i ke kikowaena.

III. Ke Keʻena Hoʻokaʻawale ʻana o ka Gradient Density:

ʻO ka centrifugation gradient density kahi ʻenehana i hoʻohana nui ʻia e hoʻokaʻawale i nā hui paʻakikī e loaʻa ana nā ʻāpana a i ʻole nā ​​​​mea o nā densities like ʻole. Ma kēia ʻano hana, hana ʻia kahi gradient density i loko o ka paipu centrifuge. Ma ka hoʻopili pono ʻana i nā hopena o nā densities like ʻole, hana ʻia kahi gradient, me ka density kiʻekiʻe loa ma lalo a me ka haʻahaʻa loa ma luna.

IV. Sedimentation a me Pelleting:

I ka hoʻokomo ʻia ʻana o ka hui ʻana i loko o ka ʻōmole centrifuge a milo ʻia i nā wikiwiki kiʻekiʻe, neʻe nā ʻāpana a i ʻole nā ​​mea ma o ka gradient density. I ka hala ʻana o ka manawa, e waiho nā ʻāpana denser a hana i kahi hui ʻike ʻia a i ʻole pellet ma lalo o ka ʻōmole. E noho mau nā ʻāpana liʻiliʻi o ka denser ma nā wahi like ʻole ma ke gradient density.

V. Nā ʻAno Centrifugation no nā noi like ʻole:

Loaʻa ka hoʻohana ʻana o ka centrifugation ma nā ʻano ʻoihana like ʻole a me nā kahua, me ka biology, kemika, ka mālama ola kino, a me nā mea hou aku. E ʻimi kākou i kekahi mau ʻano hana koʻikoʻi a me kā lākou hoʻohana ponoʻī.

A. ʻOkoʻa Centrifugation:

He ʻano hana i hoʻohana nui ʻia ka centrifugation ʻokoʻa i ka biology a me ka biochemistry. Pili ia i kahi moʻo o nā ʻanuʻu centrifugation ma nā wikiwiki a me nā lōʻihi like ʻole e hoʻokaʻawale i nā ʻāpana ma muli o ko lākou nui a me ka nui. Hiki i kēia ʻano hana ke hoʻokaʻawale i nā organelles cellular, e like me mitochondria a i ʻole lysosomes, mai nā hui paʻakikī e like me nā homogenates kiko.

B. Keʻena Hoʻokaʻawale Isopycnic:

Hoʻohana ʻia ka centrifugation Isopycnic, i ʻike ʻia hoʻi ʻo ka density gradient centrifugation me ka ʻole o kahi ʻanuʻu concentration, i ka hoʻokaʻawale ʻana o ka DNA, RNA, nā virus, a me nā ʻāpana subcellular. Hoʻomaʻalahi kēia ʻenehana i ka hoʻokaʻawale ʻana o nā waikawa nucleic kikoʻī a i ʻole nā ​​​​ʻāpana viral ma muli o ko lākou density buoyant, ʻo ia hoʻi, ko lākou density e pili ana i ka medium a puni.

C. Hoʻomākaukau Ultracentrifugation:

He ʻano hana ikaika ka hoʻomākaukau ultracentrifugation i hoʻohana ʻia i ka hoʻomaʻemaʻe ʻana a me ka hoʻokaʻawale ʻana i nā nui o nā macromolecules biological, e like me nā protein a me nā nucleic acids. Ma ke kau ʻana i ka hui ʻana i nā wikiwiki rotational kiʻekiʻe, hoʻokō ʻia ka hoʻokaʻawale ʻana ma muli o nā ʻokoʻa o ke kaumaha molekala. He kuleana koʻikoʻi kēia ʻano hana i ke aʻo ʻana a me ka hoʻomaopopo ʻana i ke ʻano a me ka hana o nā biomolecules.

D. Ka wikiwiki o ka Sedimentation:

Hoʻohana ʻia ka centrifugation wikiwiki o ka sedimentation e kālailai i ka hoʻolaha nui a me ke kaumaha molekala o nā macromolecules i loko o kahi laʻana. Ma ka nānā ʻana i nā wikiwiki o ka sedimentation o nā ʻāpana i hoʻokau ʻia i nā mana ultracentrifugal, hiki i nā mea noiʻi ke loaʻa nā ʻike waiwai i ka nui, ke ʻano, a me nā ʻano diffusion o nā ʻāpana i hoʻohui ʻia.

E. Ka Hoʻokaʻawale ʻana i ka Ultracentrifugation Analytical:

Hāʻawi ka ultracentrifugation Analytical i kahi ʻenehana ikaika e aʻo ai i nā pilina macromolecular, e wehewehe i nā pilina hoʻopaʻa, a hoʻoholo i ka stoichiometry o nā hui paʻakikī. Ma ka wehewehe ʻana i nā waiwai sedimentation a me ka diffusion o nā ʻāpana ma lalo o nā ikaika ultracentrifugal, hiki i nā mea noiʻi ke wehewehe i nā paʻakikī o nā pilina molekala.

Hopena:

He mea hana koʻikoʻi nā Centrifuges i ka noiʻi ʻepekema a me ka ʻoihana, e ʻae ana i ka hoʻokaʻawale pololei ʻana o nā hui paʻakikī. Ma ka hoʻohana ʻana i ka mana o ka ikaika centrifugal a me ka hoʻokō ʻana i nā ʻano hana like ʻole, hoʻomaʻamaʻa lākou i ka hoʻokaʻawale ʻana a me ka nānā ʻana i nā ʻāpana like ʻole e pili ana i ko lākou nui, ka nui, a me ka nui o ka lana. Me kā lākou noi ākea ma nā ʻano aʻo like ʻole, ke hoʻomau nei nā centrifuges i kahi kuleana koʻikoʻi i ka hoʻolaha ʻana i ko mākou ʻike i ke ao kūlohelohe a me ka hoʻomaikaʻi ʻana i nā kaʻina hana ʻenehana like ʻole.