Sentrifuge e arola metsoako joang

Li-Centrifuge: Ho Senola Liphiri tsa Karohano ea Motsoako

Selelekela:

Li-centrifuge ke lisebelisoa tse matla tse sebelisoang lits'ebetsong tse fapaneng tsa saense le tsa indasteri ho arola metsoako e rarahaneng ka likarolo tsa eona ka bonngoe. Lisebelisoa tsena tse ikhethang li sebelisa melao-motheo ea matla a centrifuge ho fihlela karohano e nepahetseng, li fa bafuputsi le litsebi temohisiso ea bohlokoa mabapi le mefuta e mengata ea lisebelisoa. Sehloohong sena, re tla hlahloba kamoo li-centrifuge li arolang metsoako kateng, ts'ebeliso ea eona masimong a fapaneng, le mekhoa ea motheo e etsang hore e sebetse hantle hakana.

I. Ho Utloisisa Matla a Bohareng:

Ho utloisisa hore na li-centrifuge li sebetsa joang, re tlameha ho qala ka ho teba khopolong ea matla a centrifugal. Ha ntho e potoloha axis e bohareng, joalo ka top e potolohang kapa merry-go-round, e ba le matla a hulang kantle a tsejoang e le matla a centrifugal. Matla ana a atisa ho sutumelletsa lintho hole le bohareng ba potoloho.

II. Ho Sebelisa Matla a Sekhutlo bakeng sa Karohano:

Di-Centrifuge di sebedisa matla ana a centrifugal ho arola metswako ho latela bongata bo fapaneng ba dikarolo tsa tsona. Ka ho potoloha ka lebelo le phahameng, centrifuge e hlahisa tshimo ya centrifugal e hlahisang matla a matla a kantle. Matla ana a etsa hore dikarolwana tse teteaneng kapa dintho tse ka hara motswako di tsamaye ho ya mathokong a ka ntle, ha dikarolo tse bobebe di ntse di le haufi le bohareng.

III. Ho Fokotsa Botenya ba Gradient:

Ho centrifugation ha gradient e teteaneng ke mokhoa o sebediswang haholo ho arola metswako e rarahaneng e nang le dikarolwana kapa dintho tse nang le bongata bo fapaneng. Mokgweng ona, gradient e teteaneng e etswa ka hara tube ya centrifuge. Ka ho beha ka hloko ditharollo tsa bongata bo fapaneng, gradient e a thehwa, ka bongata bo phahameng ka ho fetisisa bo le tlase mme bo tlase bo le hodimo.

IV. Ho senngoa ha mashala le ho ntsha peterole:

Ha motsoako o kenngwa ka hara tube ya centrifuge mme o ohla ka lebelo le phahameng, dikarolwana kapa dintho di tsamaya ka hara gradient ya density. Ha nako e ntse e ya, dikarolo tse teteaneng di tla qhibidiha mme di etse lebanta le bonahalang kapa pellet tlase ho tube. Dikarolo tse sa teteaneng haholo di tla dula di leketlile dintlheng tse fapaneng ho latela gradient ya density.

V. Mekhoa ea ho Centrifugation bakeng sa Litšebeliso tse Fapaneng:

Ho sebelisa centrifugation ho sebelisoa liindastering le mafapheng a fapaneng, ho kenyeletsoa baeloji, k'hemistri, tlhokomelo ea bophelo bo botle le tse ling. A re hlahlobeng mekhoa e 'maloa ea bohlokoa le ts'ebeliso ea eona.

A. Phapang ea ho Fokotsa Matla:

Ho arola dipharologanyo ke mokhoa o sebediswang haholo thutong ya baeloji le ya biochemistry. E kenyeletsa letoto la mehato ya ho arola dipharologanyo ka lebelo le dinako tse fapaneng ho arola dikarolwana ka tatellano ho latela boholo le bongata ba tsona. Mokgwa ona o nolofalletsa ho arola di-organelle tsa sele, tse kang mitochondria kapa lysosomes, ho tswa metswakong e rarahaneng jwalo ka dinama tse homogenate.

B. Ho Fokotsa ho Sebetsana ha Isopycnic:

Ho centrifugation ha Isopycnic, e tsejoang hape e le density gradient centrifugation ntle le mohato wa mahloriso, e sebediswa karohanong ya DNA, RNA, divaerase le dikarolwana tse ka tlase ho sele. Mokgwa ona o thusa ho arola di-nucleic acid tse itseng kapa dikarolwana tsa vaerase ho latela bongata ba tsona bo phaphametseng, ke hore, bongata ba tsona ha bo bapiswa le sebaka se di potileng.

C. Ho lokisa Ultracentrifugation:

Ho lokisa ultracentrifugation ke mokhoa o matla o sebelisoang ho hloekiseng le ho arola bongata bo boholo ba li-macromolecule tsa baeloji, joalo ka liprotheine le li-nucleic acid. Ka ho beha motsoako lebelo le phahameng la ho potoloha, karohano e fihlelleha ho latela phapang ea boima ba limolek'hule. Mokhoa ona o bapala karolo ea bohlokoa ho ithuteng le ho utloisiseng sebopeho le ts'ebetso ea li-biomolecule.

D. Lebelo la ho senngoa ha seretse:

Ho potoloha lebelo la sedimentation ho sebediswa ho sekaseka kabo ya boholo le boima ba dimolekhule tsa dimolekhule tse kgolo ka hara sampole. Ka ho sekaseka lebelo la sedimentation la dikarolwana tse tlasa matla a ultracentrifugal, bafuputsi ba ka fumana temohisiso ya bohlokwa mabapi le boholo, sebopeho le dibopeho tsa ho hasana ha dikarolo tsa dikarolo.

E. Tlhahlobo ea Ultracentrifugation:

Ho hlahloba ka ultracentrifugation ho fana ka mokhoa o matla oa ho ithuta likamano tsa macromolecular, ho hlalosa likamano tse tlamang, le ho fumana stoichiometry ea metsoako e rarahaneng. Ka ho sekaseka litšobotsi tsa sedimentation le ho hasana ha likaroloana tlas'a matla a ultracentrifugal, bafuputsi ba ka senola mathata a likamano tsa limolek'hule.

Qetello:

Li-centrifuge ke sesebelisoa sa bohlokoa lipatlisisong tsa mahlale le indastering, se lumellang karohano e nepahetseng ea metsoako e rarahaneng. Ka ho sebelisa matla a matla a centrifuge le ho kenya tšebetsong mekhoa e fapaneng, li nolofatsa ho aroloa le ho hlahlojoa ha likarolo tse fapaneng ho latela bongata ba tsona, boholo le bongata ba tsona bo phallang. Ka lits'ebetso tsa tsona tse pharaletseng mafapheng a mangata, li-centrifuge li ntse li tsoela pele ho bapala karolo ea bohlokoa ho ntlafatseng kutloisiso ea rona ea lefats'e la tlhaho le ho ntlafatsa lits'ebetso tse fapaneng tsa theknoloji.