Me pēhea te wehewehe i ngā ranunga mā te centrifuge

Ngā Pūwero: Te Wetewete i ngā Mea Ngaro o te Wehewehenga Ranunga

Kupu Whakataki:

He taputapu kaha ngā pūwero e whakamahia ana i roto i ngā mahi pūtaiao me ngā mahi ahumahi hei wehewehe i ngā ranunga uaua ki roto i ō rātou ake wāhanga. Ka whakamahia e ēnei taputapu whakamiharo ngā mātāpono o te kaha pūwero hei whakatutuki i te wehenga tika, e tuku ana i ngā tirohanga whai hua ki ngā kairangahau me ngā tohunga hangarau ki te whānuitanga o ngā rauemi. I roto i tēnei tuhinga, ka tūhuratia e mātou te wehewehe a ngā pūwero i ngā ranunga, ō rātou whakamahinga i roto i ngā mara rerekē, me ngā tikanga matua e whai hua ai.

I. Te Mārama ki te Kaha Pūtahi:

Hei mārama ki te mahi a ngā pūwhakatere, me ruku hohonu tātou ki te ariā o te kaha whakatere. Ina huri tetahi mea i te tuaka pokapū, pērā i te pōro hurihuri, i te meri-go-round rānei, ka pāngia e te kaha kukume ki waho e mōhiotia ana ko te kaha whakatere. Ka panaia e tēnei kaha ngā mea kia matara atu i te pokapū hurihuri.

II. Te Whakamahi i te Kaha Pūtahi mō te Wehewehenga:

Ka whakamahia e ngā pūwhakatere tēnei kaha whakatere hei wehewehe i ngā ranunga i runga i ngā momo matotoru o ō rātou wāhanga. Mā te hurihuri i ngā tere teitei, ka hangaia e te pūwhakatere he mara whakatere e whakaputa ana i tētahi kaha kaha ki waho. Mā tēnei kaha ka neke ngā matūriki, ngā matū rānei, kia matotoru ake i roto i te ranunga ki ngā taha o waho, ko ngā wāhanga māmā ake ka noho tata atu ki waenganui.

III. Te Whakapūtanga o te Rino Matotoru:

Ko te whakapūtanga i te pikinga matotoru he tikanga e whakamahia whānuitia ana hei wehewehe i ngā ranunga matatini kei roto ngā matūriki, ngā matū rānei o ngā matotoru rerekē. I roto i tēnei tikanga, ka hangaia he pikinga matotoru i roto i te ngongo whakapūtanga. Mā te whakatakoto marie i ngā otinga o ngā matotoru rerekē, ka hangaia he pikinga, ko te matotoru teitei kei raro, ko te matotoru iti kei runga.

IV. Te Whakatakotoranga me te Whakatakotoranga:

I te whakaurunga o te ranunga ki roto i te ngongo centrifuge, ā, i te mirohia i ngā tere teitei, ka heke ngā matūriki, ngā matū rānei i roto i te pikinga kiato. I roto i te wā, ka whakatōngia ngā wāhanga kiato ake, ka hanga he whitiki, he pirepire rānei e kitea ana i raro i te ngongo. Ka noho tonu ngā wāhanga iti iho te kiato i ngā wāhi rerekē i te pikinga kiato.

V. Ngā Tikanga Whakapūmau mō ngā Mahi Kanorau:

Ka kitea te whakamahinga o te centrifugation i roto i te whānuitanga o ngā ahumahi me ngā mara, tae atu ki te koiora, te matū, te hauora, me te maha atu. Me tūhuratia ētahi tikanga matua me ō rātou whakamahinga.

A. Te Whakapūtanga Rerekētanga:

He tikanga whānui te whakamahi i te centrifugation rerekē i roto i te koiora me te matū koiora. Kei roto ko te raupapa o ngā mahi centrifugation i ngā tere me ngā roa rerekē hei wehewehe i ngā matūriki i runga i te rahi me te mātotoru. Mā tēnei tikanga ka taea te wehe i ngā whekau pūtau, pērā i ngā mitochondria, i ngā lysosomes rānei, mai i ngā ranunga matatini pērā i ngā homogenates kiko.

B. Te Whakapūtanga Isopycnic:

Ko te centrifugation isopycnic, e mōhiotia ana ko te centrifugation density gradient kāore he taahiraa kukū, e whakamahia ana i roto i te wehenga o te DNA, te RNA, ngā huaketo, me ngā matūriki o roto i te pūtau. Mā tēnei tikanga ka taea te wehe i ngā waikawa karihi motuhake, i ngā matūriki huaketo rānei i runga i tō rātou kiato pōkarekare, arā, tō rātou kiato e pā ana ki te reo e karapoti ana.

C. Te Whakatakotoranga Ultracentrifugation Whakarite:

He tikanga kaha te ultracentrifugation whakarite e whakamahia ana mō te purenga me te wehe i ngā rahinga nui o ngā macromolecules koiora, pērā i ngā pūmua me ngā waikawa karihi. Mā te tuku i te ranunga ki ngā tere hurihuri teitei, ka tutuki te wehenga i runga i ngā rerekētanga o te taumaha ngota. He mea nui tēnei tikanga ki te ako me te mārama ki te hanganga me te mahi a ngā biomolecules.

D. Te Tere o te Whakatakotoranga:

Ka whakamahia te whakateretere tere o te whakatōpara hei tātari i te tohatoha rahi me te taumaha ngota o ngā macromolecules i roto i tētahi tauira. Mā te tātari i ngā tere whakatōpara o ngā matūriki i pāngia e ngā kaha whakateretere-rahi, ka taea e ngā kairangahau te whiwhi māramatanga whai hua mō te rahi, te āhua, me ngā āhuatanga horapa o ngā wāhanga o roto.

E. Te Whakapūtanga Tino-ā-Tatari:

Mā te tātaritanga ultracentrifugation he tikanga kaha hei ako i ngā taunekeneke macromolecular, hei whakaahua i ngā hononga here, me te whakatau i te stoichiometry o ngā ranunga matatini. Mā te tātari i ngā āhuatanga o te whakatō me te horapa o ngā matūriki i raro i ngā kaha ultracentrifugal, ka taea e ngā kairangahau te wetewete i ngā uauatanga o ngā taunekeneke ngota.

Whakatau:

He taputapu tino nui ngā pūwero i roto i te rangahau pūtaiao me te ahumahi, e āhei ai te wehewehe tika i ngā ranunga uaua. Mā te whakamahi i te kaha o te pūwero me te whakatinana i ngā tikanga rerekē, ka āwhina i te wehewehe me te tātari i ngā wāhanga rerekē i runga i tō rātou kiato, rahi, me te kiato pōkarekare. Me ō rātou whakamahinga whānui puta noa i ngā momo kaupapa, kei te whai wāhi tonu ngā pūwero ki te whakatairanga i tō tātou māramatanga ki te ao tūroa me te whakarei ake i ngā tukanga hangarau.