Centrifuge inoparadzanisa sei musanganiswa

Centrifuges: Kufumura Zvakavanzika zvekuparadzaniswa kweMusanganiswa

Nhanganyaya:

MaCentrifuge zvishandiso zvine simba zvinoshandiswa mumabasa akasiyana-siyana esainzi nemaindasitiri kupatsanura misanganiswa yakaoma kuita zvikamu zvayo. Midziyo iyi inoshamisa inoshandisa misimboti yesimba recentrifugal kuti iwane kupatsanurana kwakanyatsojeka, ichipa vaongorori nenyanzvi ruzivo rwakakosha nezvezvinhu zvakasiyana-siyana. Muchinyorwa chino, tichaongorora kuti maCentrifuge anoparadzanisa sei misanganiswa, mashandisirwo ayo muminda yakasiyana, uye nzira dzinoita kuti ishande zvakanaka.

I. Kunzwisisa Simba reCentrifugal:

Kuti tinzwisise mashandiro anoita ma centrifuge, tinofanira kutanga taongorora pfungwa ye centrifugal force. Kana chinhu chikatenderera pakati pechinhu, senge top inotenderera kana merry-go-round, chinosangana nesimba rinodhonza kunze rinozivikanwa se centrifugal force. Simba iri rinowanzo sundira zvinhu kure nepakati pekutenderera.

II. Kushandisa Simba reCentrifugal pakuparadzanisa:

MaCentrifuge anoshandisa simba iri recentrifugal kuparadzanisa musanganiswa zvichienderana nekuwanda kwezvikamu zvavo. Nekutenderera nekumhanya kwakanyanya, centrifuge inogadzira munda wecentrifugal unogadzira simba guru rekunze. Simba iri rinoita kuti zvidimbu kana zvinhu zviri mumusanganiswa zvifambe zvichienda kumucheto wekunze, nepo zvikamu zvakareruka zvichiramba zviri pedyo nepakati.

III. Kudzora Kureba Kwemagiraidhi Ekudzikisa Huremu:

Kuyera density gradient centrifugation inzira inoshandiswa zvakanyanya kuparadzanisa misanganiswa yakaoma ine zvidimbu kana zvinhu zvine density dzakasiyana. Munzira iyi, density gradient inogadzirwa mukati mechubhu yecentrifuge. Nekunyatsoisa mhinduro dze density dzakasiyana, gradient inoumbwa, density yepamusoro iri pasi uye yakaderera iri pamusoro.

IV. Kuchera nekubvisa tsvina:

Sezvo musanganiswa wacho uchiiswa muchubhu yecentrifuge uye uchitenderedzwa nekumhanya kwakanyanya, zvidimbu kana zvinhu zvinofamba nepakati pe density gradient. Nekufamba kwenguva, zvikamu zvakawandisa zvinodonha zvoita band kana pellet inooneka pasi pechubhu. Zvikamu zvisina density zvinoramba zvakamira panzvimbo dzakasiyana zvichienderana ne density gradient.

V. Nzira dzeCentrifugation dzeMashandisirwo Akasiyana-siyana:

Kuchenesa masero emuchina (centrifugation) kunoshandiswa mumaindasitiri akasiyana-siyana, kusanganisira biology, chemistry, hutano, nezvimwewo. Ngationgororei nzira dzinokosha shoma uye mashandisirwo adzo.

A. Kusiyanisa kweCentrifugation:

Kuparadzanisa masero (differential centrifugation) inzira inoshandiswa zvakanyanya mu biology ne biochemistry. Inosanganisira matanho akasiyana-siyana e centrifugation nekumhanya kwakasiyana uye nguva dzakasiyana kuti uparadzanise zvidimbu zvakatevedzana zvichienderana nehukuru hwazvo uye huwandu hwazvo. Nzira iyi inobvumira kupatsanurwa kwemasero e organelles, akadai se mitochondria kana lysosomes, kubva mumisanganiswa yakaoma senge homogenates dzematishu.

B. Kudzora Kushanda Kwemasero muIsopycnic:

Isopycnic centrifugation, inozivikanwawo se density gradient centrifugation isina danho re concentration, inoshandiswa mukuparadzanisa DNA, RNA, mavhairasi, uye subcellular particles. Iyi nzira inobatsira kupatsanura ma nucleic acids chaiwo kana mavhairasi zvichienderana nehuwandu hwawo hwe buoyant, kureva, huwandu hwawo kana tichienzanisa nenzvimbo yakapoteredza.

C. Kugadzirira Ultracentrifugation:

Kugadzirira ultracentrifugation inzira ine simba inoshandiswa mukuchenesa nekuparadzanisa huwandu hwakawanda hwemacromolecules ehupenyu, akadai semapuroteni nema nucleic acids. Nekuisa musanganiswa kumhanya kwakanyanya, kupatsanurwa kunowanikwa zvichibva pakusiyana kwehuremu hwemamorekuru. Nzira iyi inoita basa guru mukudzidza nekunzwisisa chimiro nebasa remabiomolecules.

D. Kumhanya Kwekudzura:

Kushandiswa kwe sedimentation velocity centrifugation kunoshandiswa kuongorora kugoverwa kwehukuru uye huremu hwemamorekuru e macromolecules mumuenzaniso. Nekuongorora kumhanya kwe sedimentation kwezvikamu zvakashandiswa nesimba re ultracentrifugal, vaongorori vanogona kuwana ruzivo rwakakosha pamusoro pehukuru, chimiro, uye hunhu hwekupararira kwezvikamu zvirimo.

E. Kuongorora Ultracentrifugation:

Kuongororwa kwe ultracentrifugation kunopa nzira ine simba yekudzidza kupindirana kwema macromolecular, kuratidza hukama hwekubatanidza, uye kuona stoichiometry yemisanganiswa yakaoma. Nekuongorora hunhu hwe sedimentation uye diffusion yezvikamu pasi pesimba re ultracentrifugal, vaongorori vanogona kuburitsa pachena kuoma kwekudyidzana kwema molecular.

Mhedziso:

MaCentrifuge chishandiso chakakosha mukutsvagisa kwesainzi uye mumaindasitiri, zvichibvumira kupatsanurwa kwakanyatsojeka kwemisanganiswa yakaoma. Nekushandisa simba resimba recentrifugal uye kushandisa matekiniki akasiyana-siyana, zvinoita kuti zvive nyore kupatsanura nekuongorora zvikamu zvakasiyana-siyana zvichienderana nekuwanda kwazvo, saizi, uye kusimba kwazvinoita. Nekushandiswa kwazvo kwakasiyana-siyana muzvikamu zvakasiyana-siyana, maCentrifuge anoramba achiita basa guru mukusimudzira kunzwisisa kwedu nyika yechisikigo uye kusimudzira maitiro akasiyana-siyana etekinoroji.