indlela yokuhlukanisa usawoti namanzi usebenzisa i-centrifuge

Isingeniso

I-Centrifugation iyindlela esetshenziswa kabanzi ezinhlotsheni eziningi zesayensi nezimboni. Ihilela ukuhlukaniswa kwezingxenye engxubeni ngokusekelwe ekubuneni kwazo okuhlukene. Enye indlela ewusizo yokusebenzisa i-centrifugation ukuhlukaniswa kosawoti namanzi. Kulesi sihloko, sizohlola indlela yokuhlukanisa usawoti namanzi ngempumelelo sisebenzisa i-centrifuge. Sizoxoxa ngesimiso ngemuva kwe-centrifugation, imishini edingekayo, kanye nenqubo yesinyathelo ngesinyathelo yokuhlukanisa usawoti namanzi kusetshenziswa le ndlela.

I. Ukuqonda i-Centrifugation

I-Centrifugation isekelwe esimisweni se-sedimentation, okuyinqubo yokuhlala phansi kwezinhlayiya eziqinile oketshezini ngenxa yamandla adonsela phansi. I-centrifuge isebenzisa ukunyakaza kokujikeleza ukwandisa amandla adonsela phansi asebenza engxubeni, ngaleyo ndlela isheshise inqubo yokuhlukanisa. Esimweni sokuhlukaniswa kosawoti namanzi, amandla e-centrifugal azosiza ekudonseni izinhlayiya zikasawoti kude namanzi, okwenza kube lula ukuhlukana kwazo.

II. Imishini Edingekayo

Ukuze uhlukanise usawoti namanzi usebenzisa i-centrifuge, uzodinga imishini elandelayo:

1. I-Centrifuge: Kuyadingeka ukuthi ube nokufinyelela ku-centrifuge yezinga lelebhu. I-centrifuge kufanele ibe nezinketho ezifanele zokulawula isivinini kanye nokushisa ukuze ihlukaniswe ngempumelelo.

2. Amapayipi E-Centrifuge: Lawa mapayipi aklanyelwe ngqo ukumelana namandla aphezulu e-centrifugal futhi abalulekile ekuhlanganiseni ingxube ngesikhathi senqubo. Qinisekisa ukuthi amapayipi avalwe kahle ukuze kugwenywe noma yikuphi ukuvuza.

3. Ama-pipettes: Ama-pipettes ayadingeka ukuze kudluliselwe ingxube ngokunembile kumashubhu e-centrifuge.

4. Isixazululo Sikasawoti Namanzi: Lungisa ukuhlushwa okwaziwayo kwesixazululo sikasawoti namanzi. Usawoti kufanele uncibilikiswe ngokuphelele emanzini ngaphambi kokuqala inqubo yokuhlukanisa.

III. Inqubo Yesinyathelo Ngesinyathelo

Manje, ake sihambe ngenqubo yesinyathelo ngesinyathelo yokuhlukanisa usawoti namanzi sisebenzisa i-centrifuge:

Isinyathelo 1: Gcwalisa amashubhu e-centrifuge

Usebenzisa ipayipi, dlulisela ngokucophelela isisombululo sikasawoti namanzi emapayipini e-centrifuge. Qiniseka ukuthi ushiya isikhala esithile epayipini ukuze uvimbele ukugcwala ngesikhathi se-centrifuge.

Isinyathelo 2: Linganisa amashubhu

Kubalulekile ukuqinisekisa ukuthi amashubhu alingana ngokulinganayo ku-centrifuge rotor ukuze kuvinjelwe noma yikuphi ukuphazamiseka ekujikelezeni. Lungisa indawo yamashubhu noma wengeze izinto eziphikisanayo uma kudingeka.

Isinyathelo 3: Setha amapharamitha e-centrifuge

Bheka imiyalelo yomenzi yokusetha isivinini esifanele se-centrifuge kanye nesikhathi esidingekayo sokuhlukaniswa kukasawoti namanzi. Isivinini kufanele sanele ukukhiqiza amandla aphezulu e-centrifugal.

Isinyathelo 4: Qala i-centrifuge

Beka amashubhu ngokucophelela ku-rotor ye-centrifuge, uqinisekise ukuthi aboshwe kahle. Vala isivalo bese uqala i-centrifuge ngokwemigomo ebekiwe.

Isinyathelo 5: Linda ukuthi i-centrifugation iqede

Vumela i-centrifuge ukuthi isebenze isikhathi esibekiwe. Phakathi nalesi sikhathi, amandla e-centrifugal azobangela ukuthi izinhlayiya zikasawoti zibe yizicucu phansi kwepayipi kuyilapho amanzi ehlala engxenyeni engenhla.

Isinyathelo 6: Misa i-centrifuge bese uqoqa izingxenye ezihlukanisiwe

Uma i-centrifugation isiqediwe, susa ngokucophelela amapayipi ku-rotor bese umisa i-centrifuge. Ungabe usuhlukanisa usawoti ngokususa amanzi angaphezulu noma ngokusebenzisa i-pipette ukukhipha amanzi, ushiye inhlabathi kasawoti.

IV. Isiphetho

I-Centrifugation iyindlela enamandla yokuhlukanisa izingxenye ngengxube ngokusekelwe ekubuneni kwazo okuhlukile. Ngokusebenzisa i-centrifuge, kungenzeka ukuhlukanisa usawoti emanzini ngempumelelo. Ukuqonda izimiso ezingemuva kwe-centrifugation, ukuthola imishini edingekayo, nokulandela inqubo yesinyathelo ngesinyathelo kuzokwenza kube lula ukuhlukana okuphumelelayo. Le ndlela ithola ukubaluleka ezimbonini ezahlukene, njengezemithi, isayensi yezemvelo, kanye nokukhiqizwa kwamakhemikhali, lapho ukuhlukaniswa kosawoti namanzi kubalulekile khona ezinhlotsheni ezahlukene zokusebenza.