mokhoa oa ho arola letsoai le metsi ka ho sebelisa centrifuge

Selelekela

Ho centrifugation ke mokhoa o sebelisoang haholo lits'ebetsong tse ngata tsa saense le tsa indasteri. E kenyelletsa karohano ea likarolo ka motsoako ho latela bongata ba tsona bo fapaneng. Tšebeliso e 'ngoe e sebetsang ea ho centrifugation ke karohano ea letsoai le metsi. Sehloohong sena, re tla hlahloba mokhoa oa ho arola letsoai le metsi ka katleho re sebelisa centrifuge. Re tla buisana ka molao-motheo o ka morao ho centrifugation, lisebelisoa tse hlokahalang, le ts'ebetso ea mohato ka mohato ea ho arola letsoai le metsi ho sebelisa mokhoa ona.

I. Ho utloisisa Centrifugation

Ho centrifugation ho itshetlehile hodima molao-motheo wa ho sedimentation, e leng tshebetso ya ho dula ha dikarolwana tse tiileng ka hara mokelikeli ka lebaka la matla a kgoheli. Centrifuge e sebedisa motsamao wa ho potoloha ho eketsa matla a kgoheli a sebetsang motswakong, ka hona ho potlakisa tshebetso ya karohano. Moelelong wa karohano ya letswai le metsi, matla a centrifugal a tla thusa ho hula dikarolwana tsa letswai hole le metsi, ho nolofatsa karohano ya tsona.

II. Lisebelisoa tse Hlokahalang

Ho arola letsoai le metsi ka centrifuge, o tla hloka lisebelisoa tse latelang:

1. Sentifuge: Hoa hlokahala ho ba le phihlello ea sentifuge ea boemo ba laboratori. Sentifuge e lokela ho ba le likhetho tse loketseng tsa taolo ea lebelo le mocheso bakeng sa karohano e sebetsang.

2. Liphaephe tsa Centrifuge: Liphaephe tsena li etselitsoe ka ho khetheha ho mamella matla a phahameng a centrifugal 'me li bohlokoa bakeng sa ho boloka motsoako nakong ea ts'ebetso. Netefatsa hore liphaephe li koetsoe hantle ho qoba ho lutla ho hong.

3. Li-pipettes: Li-pipettes lia hlokahala bakeng sa ho fetisetsa motsoako ka nepo ka har'a liphaephe tsa centrifuge.

4. Tharollo ea Letsoai le Metsi: Lokisetsa motsoako o tsebahalang oa tharollo ea letsoai le metsi. Letsoai le lokela ho qhibiliha ka botlalo ka metsing pele le qala ts'ebetso ea karohano.

III. Tsamaiso ea Mohato ka Mohato

Jwale, ha re tsamayeng mohato ka mohato ka mohato ka mohato wa ho arola letswai le metsi re sebedisa centrifuge:

Mohato oa 1: Tlatsa liphaephe tsa centrifuge

U sebelisa pipette, fetisetsa tharollo ea letsoai le metsi ka hloko ka har'a lipeipi tsa centrifuge. Etsa bonnete ba hore u siea sebaka se itseng ka har'a tube ho thibela ho tlala ha metsi nakong ea centrifuge.

Mohato oa 2: Leka-lekanya liphaephe

Ho bohlokoa ho netefatsa hore lipeipi li leka-lekane ka ho lekana ka har'a rotor ea centrifuge ho thibela tšitiso efe kapa efe motsamaong oa ho potoloha. Fetola boemo ba lipeipi kapa eketsa lintho tse ling tse hanyetsanang haeba ho hlokahala.

Mohato oa 3: Beha liparamente tsa centrifuge

Sheba litaelo tsa moetsi bakeng sa ho beha lebelo le nepahetseng la centrifuge le nako e hlokahalang bakeng sa karohano ea letsoai le metsi. Lebelo le lokela ho lekana ho hlahisa matla a phahameng a centrifugal.

Mohato oa 4: Qala centrifuge

Beha lipeipi ka hloko ka har'a rotor ea centrifuge, u netefatse hore li tlamiloe hantle. Koala sekoahelo 'me u qale centrifuge ho latela liparamente tse behiloeng.

Mohato oa 5: Emela hore centrifugation e phethe

Lumella centrifuge hore e sebetse ka nako e behiloeng. Nakong ena, matla a centrifugal a tla etsa hore likaroloana tsa letsoai li qhalane ka tlase ho tube ha metsi a ntse a le karolong e ka holimo.

Mohato oa 6: Emisa centrifuge 'me u bokelle likarolo tse arohaneng

Hang ha ho centrifugation ho phethetswe, tlosa diphaephe ka hloko ho rotor mme o emise centrifuge. Ebe o ka arola letswai ka ho ntsha metsi a matla kapa ka ho sebedisa pipette ho ntsha metsi, o siee letswai le lenyenyane.

IV. Qetello

Ho centrifugation ke mokhoa o matla oa ho arola likarolo ka motsoako ho latela bongata ba tsona bo fapaneng. Ka ho sebelisa centrifuge, hoa khoneha ho arola letsoai le metsi ka katleho. Ho utloisisa melao-motheo e ka morao ho centrifugation, ho fumana lisebelisoa tse hlokahalang, le ho latela mokhoa oa mohato ka mohato ho tla nolofalletsa karohano e atlehileng. Mokhoa ona o fumana bohlokoa liindastering tse fapaneng, tse kang meriana, mahlale a tikoloho le tlhahiso ea lik'hemik'hale, moo karohano ea letsoai le metsi e leng ea bohlokoa bakeng sa lits'ebetso tse fapaneng.