yuav ua li cas cais cov ntsev thiab dej siv centrifuge
Kev Taw Qhia
Kev siv centrifugation yog ib txoj kev siv dav hauv ntau yam kev tshawb fawb thiab kev lag luam. Nws cuam tshuam nrog kev sib cais cov khoom sib xyaw ua ke raws li lawv qhov sib txawv. Ib qho kev siv centrifugation yog kev sib cais ntsev thiab dej. Hauv tsab xov xwm no, peb yuav tshawb nrhiav yuav ua li cas cais ntsev ntawm dej siv lub centrifuge. Peb yuav tham txog lub hauv paus ntsiab lus tom qab centrifugation, cov khoom siv xav tau, thiab cov txheej txheem ib kauj ruam zuj zus ntawm kev sib cais ntsev thiab dej siv cov txheej txheem no.
I. Kev Nkag Siab Txog Kev Centrifugation
Kev siv lub tshuab centrifugation yog ua raws li lub hauv paus ntsiab lus ntawm kev tso dej, uas yog cov txheej txheem ntawm kev tso cov khoom khov rau hauv cov kua vim yog lub ntiajteb txawj nqus. Lub tshuab centrifuge siv lub zog tig los ua kom lub zog gravitational ua rau cov khoom sib xyaw, yog li ua kom cov txheej txheem sib cais sai dua. Hauv cov ntsiab lus ntawm kev sib cais ntsev thiab dej, lub zog centrifugal yuav pab rub cov khoom ntsev tawm ntawm dej, ua kom lawv sib cais yooj yim.
II. Cov Khoom Siv Yuav Tsum Tau Muaj
Yuav kom ua tiav kev sib cais ntsev thiab dej siv lub centrifuge, koj yuav xav tau cov khoom siv hauv qab no:
1. Centrifuge: Yuav tsum muaj lub centrifuge uas siv tau rau hauv chav kuaj. Lub centrifuge yuav tsum muaj qhov ceev thiab qhov kub kom haum rau kev sib cais kom zoo.
2. Cov Hlab Centrifuge: Cov hlab no yog tsim tshwj xeeb los tiv taus lub zog centrifugal siab thiab yog qhov tseem ceeb rau kev ntim cov khoom sib tov thaum lub sijhawm ua haujlwm. Xyuas kom tseeb tias cov hlab tau kaw kom zoo kom tsis txhob muaj kev xau.
3. Cov Pipettes: Cov Pipettes yog qhov yuav tsum tau muaj rau kev xa cov khoom sib xyaw rau hauv cov raj centrifuge kom raug.
4. Cov Tshuaj Ntshav thiab Dej: Npaj cov tshuaj ntsev thiab dej uas paub tias muaj pes tsawg. Cov ntsev yuav tsum yaj tag rau hauv dej ua ntej pib ua cov txheej txheem sib cais.
III. Cov Kauj Ruam Ib Kauj Ruam
Tam sim no, cia peb taug kev los ntawm cov txheej txheem ib kauj ruam zuj zus ntawm kev sib cais ntsev thiab dej siv lub centrifuge:
Kauj Ruam 1: Sau cov raj centrifuge
Siv lub raj xa dej, ua tib zoo muab cov ntsev thiab cov dej tso rau hauv cov raj centrifuge. Nco ntsoov tseg qee qhov chaw hauv lub raj kom tiv thaiv kev ntws dhau thaum lub sijhawm centrifuge.
Kauj Ruam 2: Sib npaug cov raj
Nws yog ib qho tseem ceeb heev uas yuav tsum xyuas kom meej tias cov raj muaj kev sib npaug zoo hauv lub centrifuge rotor kom tiv thaiv kev cuam tshuam rau kev tig. Kho qhov chaw ntawm cov raj lossis ntxiv cov khoom hnyav yog tias xav tau.
Kauj Ruam 3: Teem cov kev teeb tsa centrifuge
Saib cov lus qhia ntawm lub chaw tsim khoom kom paub txog kev teeb tsa qhov ceev thiab lub sijhawm uas yuav tsum tau siv rau kev sib cais ntsev thiab dej. Qhov ceev yuav tsum txaus los tsim kom muaj lub zog centrifugal siab dua.
Kauj Ruam 4: Pib lub centrifuge
Ua tib zoo muab cov raj tso rau hauv lub rotor centrifuge, xyuas kom lawv tau khi ruaj khov. Kaw lub hau thiab pib lub centrifuge raws li cov kev teeb tsa.
Kauj Ruam 5: Tos kom centrifugation ua tiav
Cia lub centrifuge khiav rau lub sijhawm teev tseg. Thaum lub sijhawm no, lub zog centrifugal yuav ua rau cov ntsev me me poob rau hauv qab ntawm lub raj thaum cov dej tseem nyob rau sab saud.
Kauj Ruam 6: Nres lub centrifuge thiab sau cov khoom sib cais
Thaum lub centrifugation tiav lawm, ua tib zoo tshem cov raj ntawm lub rotor thiab nres lub centrifuge. Tom qab ntawd koj tuaj yeem cais cov ntsev los ntawm kev decanting cov dej supernatant lossis los ntawm kev siv lub pipette los rho cov dej, tawm hauv qab cov ntsev av.
IV. Xaus Lus
Kev siv lub tshuab centrifugation yog ib txoj kev siv zog heev rau kev sib cais cov khoom sib xyaw ua ke raws li lawv qhov sib txawv. Los ntawm kev siv lub tshuab centrifuge, nws muaj peev xwm cais cov ntsev ntawm dej tau zoo. Kev nkag siab txog cov ntsiab cai tom qab kev centrifugation, kev tau txais cov khoom siv tsim nyog, thiab kev ua raws li cov txheej txheem ib kauj ruam zuj zus yuav ua rau kev sib cais tau zoo. Txoj kev no pom tias muaj txiaj ntsig zoo hauv ntau yam lag luam, xws li tshuaj, kev tshawb fawb ib puag ncig, thiab kev tsim tshuaj lom neeg, qhov twg kev sib cais ntsev thiab dej yog qhov tseem ceeb rau ntau yam kev siv.
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