Me pēhea te wehewehe i te tote me te wai mā te whakamahi i te centrifuge
Kupu Whakataki
He tikanga whānui te whakamahi i te centrifugation i roto i ngā mahi pūtaiao me ngā mahi ahumahi. Kei roto i tēnei ko te wehewehe i ngā wāhanga o roto i te ranunga i runga i ō rātou matotoru rerekē. Ko tētahi whakamahinga mahi o te centrifugation ko te wehenga o te tote me te wai. I roto i tēnei tuhinga, ka tūhuratia e mātou me pēhea te wehewehe pai i te tote mai i te wai mā te whakamahi i te centrifuge. Ka matapakihia e mātou te mātāpono kei muri i te centrifugation, ngā taputapu e hiahiatia ana, me te tukanga taahiraa-i-te-taahiraa o te wehewehe i te tote me te wai mā te whakamahi i tēnei tikanga.
I. Te Mārama ki te Whakapūngao
Ko te tikanga o te centrifugation ko te whakatōtō, arā, ko te tukanga o te taunga o ngā matūriki totoka ki roto i te wai nā te kaha o te kaha ā-papa. Ka whakamahia e te centrifuge te nekehanga hurihuri hei whakanui ake i te kaha ā-papa e pā ana ki te ranunga, ā, ka tere ake te tukanga wehenga. I roto i te horopaki o te wehenga o te tote me te wai, ka āwhina te kaha centrifuge ki te kukume i ngā matūriki tote mai i te wai, ka āwhina i tō rātou wehenga.
II. Ngā Taputapu e Hiahiatia ana
Hei wehe i te tote me te wai mā te whakamahi i te centrifuge, ka hiahiatia ngā taputapu e whai ake nei:
1. Pūwero: Me whai wāhi atu ki tētahi pūwero kounga taiwhanga. Me whai kōwhiringa whakahaere tere me te pāmahana tika te pūwero kia whai hua ai te wehenga.
2. Ngā Ngongo Whakapūtāhui: He mea hanga motuhake ēnei ngongo kia tū atu ki ngā kaha pūtāhui teitei, ā, he mea nui hoki kia mau tonu te ranunga i te wā e mahi ana. Me whakarite kia hiritia pai ngā ngongo kia kore ai e turuturu.
3. Ngā Paipa: E hiahiatia ana ngā paipa hei whakawhiti tika i te ranunga ki roto i ngā ngongo centrifuge.
4. Otinga Tote me te Wai: Whakaritea he kukū e mōhiotia ana o te tote me te wai. Me rewa katoa te tote ki roto i te wai i mua i te tīmatanga o te tukanga wehewehe.
III. Tukanga Hipanga-i-te-Taumata
Nā, me haere tātou i roto i te tukanga taahiraa-i-te-taahiraa o te wehewehe i te tote me te wai mā te whakamahi i te centrifuge:
Hipanga 1: Whakakīia ngā ngongo centrifuge
Mā te whakamahi i tētahi pipette, āta whakawhitia te tote me te wai ki roto i ngā ngongo centrifuge. Kia mahara kia waiho he āputa i roto i te ngongo hei ārai i te waipuke i te wā e centrifuge ana.
Hipanga 2: Tauritehia ngā ngongo
He mea nui kia taurite te taurite o ngā ngongo i roto i te rotor centrifuge kia kore ai e whakararuraru i te nekehanga hurihuri. Whakatikatikahia te tūnga o ngā ngongo, tāpirihia rānei he taumaha taurite mēnā e tika ana.
Hipanga 3: Tautuhia ngā tawhā centrifuge
Tirohia ngā tohutohu a te kaihanga mō te whakarite i te tere tika o te pūwero me te wā e hiahiatia ana mō te wehenga o te tote me te wai. Me rawaka te tere hei whakaputa i te kaha pūwero teitei ake.
Hipanga 4: Tīmatahia te centrifuge
Āta whakanohoia ngā ngongo ki roto i te rotor centrifuge, kia mau tonu te mau. Katia te taupoki, ka tīmata te centrifuge kia rite ki ngā tawhā kua whakaritea.
Hipanga 5: Taria kia oti te centrifugation
Tukua te pūwero kia rere mō te wā kua tohua. I tēnei wā, ka meinga e te kaha pūwero kia takoto ngā matūriki tote ki raro o te ngongo, engari ka noho tonu te wai ki te taha o runga.
Hipanga 6: Whakamutua te centrifuge ka kohia ngā wāhanga kua wehea
Kia oti te whakapūpū, tangohia marietia ngā ngongo mai i te rotor, kātahi ka whakamutua te whakapūpū. Kātahi ka taea te wehewehe i te tote mā te ringihia te wai i runga ake, mā te whakamahi rānei i te pipette hei tango i te wai, kia waiho ai te paru tote.
IV. Whakamutunga
He tikanga kaha te whakamahi i te centrifugation hei wehewehe i ngā wāhanga o te ranunga i runga i ō rātou matotoru rerekē. Mā te whakamahi i te centrifuge, ka taea te wehewehe pai i te tote mai i te wai. Mā te mārama ki ngā mātāpono o te centrifugation, te whiwhi i ngā taputapu e tika ana, me te whai i tētahi tukanga taahiraa-i-te-taahiraa ka taea te wehewehe angitu. Ka kitea te hiranga o tēnei tikanga i roto i ngā ahumahi maha, pērā i te rongoā, ngā pūtaiao taiao, me te hanga matū, he mea nui te wehenga o te tote me te wai mō ngā tono rerekē.
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