loluphi uhlobo lwento enokwahlulwa kusetyenziswa i-centrifuge
Izinto ezahlulwe kusetyenziswa iCentrifuge: Indlu enamandla yokwahlula ngokuchanekileyo
Intshayelelo kwiCentrifugation kunye nezicelo zayo
I-Centrifugation yindlela esetyenziswa kakhulu kwiinkqubo ezahlukeneyo zesayensi nezoshishino ukwahlula izinto ngokusekelwe kubuninzi bazo kunye nobukhulu bazo. Le ndlela inamandla ixhomekeke ekusetyenzisweni kwamandla e-centrifugal, aveliswa ngokujikelezisa isampuli ngesantya esiphezulu, ukuze kufezekiswe ukwahlukana okusebenzayo. Ukususela kwiisampulu zebhayoloji kwiilabhoratri ukuya kwimveliso yemveliso eyahlukeneyo kwimizi-mveliso, ii-centrifuge zifumana usetyenziso kwiindawo ezahlukeneyo. Kweli nqaku, siza kuhlola iintlobo zezinto ezinokwahlulwa kusetyenziswa i-centrifuge kunye nokubaluleka kwazo kumacandelo ahlukeneyo.
Ukwahlula iiSeli kunye neeNgcawu
Enye yeendlela eziphambili zokusebenzisa i-centrifugation kukwahlula iiseli kunye namasuntswana. Ngokusebenzisa umahluko ngobukhulu kunye noxinano, i-centrifugation ivumela ukwahlulwa kwezinto ezithile kwiisampuli ezintsonkothileyo. Umzekelo, kwiilabhoratri zonyango, ii-centrifuges zisetyenziswa kakhulu ukwahlula nokufunda iiseli zegazi. Xa isampuli yegazi ijikeleziswa kwi-centrifuge ngesantya esiphezulu, iiseli ezibomvu zegazi ezinzima zihlala ezantsi ngelixa i-plasma elula ihlala phezulu, iququzelela uhlalutyo olongezelelweyo.
Ngokufanayo, kuphando lwe-microbiology, i-centrifugation idlala indima ebalulekileyo ekufumaneni iinkcubeko ezicocekileyo zebhaktheriya kunye negwele. Ngokulawula ngononophelo isantya kunye nobude be-centrifugation, ezi ntsholongwane zinokwahlulwa kwiindawo ezingqongileyo, zincede ekufundeni iimpawu zazo, imetabolism, kunye neepateni zokukhula.
Ukwahlula ngokuchanekileyo kwe-DNA kunye ne-RNA
I-Centrifugation ibalulekile ekuhlukaniseni ii-nucleic acids, ezifana ne-DNA kunye ne-RNA, kwiisampulu zebhayoloji. Ezi zinto ze-genetic zihlala zifuna ukukhutshwa ngendlela yazo ecocekileyo kwiindlela ezahlukeneyo zokusetyenziswa, kubandakanya uphando lwe-genetic kunye neenjongo zokuxilonga. Iindlela ze-Centrifugation, ezifana ne-density gradient centrifugation, zivumela ukwahlukana kwee-nucleic acids kwezinye izinto zeseli, ezifana neeproteni kunye nee-lipids.
Le nkqubo ibandakanya ukulayisha isampuli yebhayoloji kwi-density gradient medium, ngesiqhelo isisombululo se-sucrose okanye i-cesium chloride, kunye nokuyifaka nge-centrifuge kusetyenziswa isantya esiphezulu. Ngexesha le-centrifugation, ii-nucleic acids zihamba nge-density gradient zide zifike kwindawo enoxinano oluthile oluhambelana nolwazo. Le ndlela yokwahlula ngokuchanekileyo ivumela ukukhutshwa kwe-DNA ecocekileyo kunye ne-RNA kwizicelo ezisezantsi ezifana ne-PCR, ulandelelwano lwezakhi zofuzo, kunye nohlalutyo lokubonakaliswa kwezakhi zofuzo.
Ukucoca iiBiomolecules
Ukucoca i-centrifugation kubalulekile ekucoceni ii-biomolecules ezahlukeneyo, kuquka iiproteni kunye nee-enzymes. Kwiilebhu ze-biochemistry kunye ne-biotechnology, iintlobo ezahlukeneyo ze-centrifuges zisetyenziswa ukwahlula nokucoca ezi molekyuli zintsonkothileyo. Ngokusebenzisa iipropati zazo ezahlukeneyo, ezinje ngobukhulu, imilo, kunye noxinano, iindlela zokucoca i-centrifugation zisusa ngokufanelekileyo ukungcola kwaye zigxininise i-biomolecule efunekayo.
I-Ultracentrifugation, indlela enamandla esebenzisa isantya esiphezulu kakhulu, idla ngokusetyenziswa ekucoceni nasekuchazeni iiproteni. Kule nkqubo, iisampuli ziphantsi kwamandla anamandla aphindwe kangamawaka kunomxhuzulane woMhlaba, nto leyo ekhokelela ekwahlulweni ngokuchanekileyo kweenxalenye ezahlukeneyo zeproteni. Oku kwenza abaphandi bakwazi ukwahlula iiproteni ezithile okanye iiproteni ezinomdla, nto leyo eququzelela uphando olunzulu lwezakhiwo zazo zeemolekyuli kunye nemisebenzi yazo.
Izicelo ze-Centrifugation kwiShishini
Ngaphandle kophando lwesayensi, i-centrifuge idlala indima ebalulekileyo kwiinkqubo ezininzi zoshishino. Kwishishini lokutya neziselo, ii-centrifuge zisetyenziselwa ukwahlula izinto eziqinileyo kwizinto ezimanzi, ukugxila kwijusi yeziqhamo, ukucacisa iwayini, kunye nokucacisa iioyile, phakathi kwezinye iindlela zokusebenza. Ngokususa ukungcola, okufana nezinto eziqinileyo, amasuntswana axhonyiweyo, okanye iiseli ze-microbial ezingafunekiyo, ii-centrifuge zinceda ukuphucula umgangatho wemveliso kunye nokunyusa isivuno.
Ishishini lamayeza lixhomekeke kakhulu kwi-centrifuge kwiinkqubo zokwahlula nokucoca ngexesha lokuveliswa kwamayeza kunye neegonyo. Ii-centrifuge zisetyenziswa ekukhupheni izithako ezisebenzayo kwizityalo, ekuvuneni iiseli, ekucoceni iiproteni, nasekuveliseni iigonyo.
Isiphelo:
I-Centrifugation yindlela eguquguqukayo enika ukwahlula ngokuchanekileyo izinto ezahlukeneyo ngokusekelwe kubuninzi bazo kunye nobukhulu bazo. Ukusuka ekuhlukaniseni iinxalenye zegazi kwiilabhoratri zonyango ukuya ekuhlukaniseni iiproteni zophando kunye nokucoca iikhompawundi zamayeza, ii-centrifuge zizixhobo ezibalulekileyo kwiintlobo ngeentlobo zezicelo. Njengoko ubuchwepheshe buqhubela phambili, iindlela ze-centrifugation ziyaqhubeka nokukhula kwaye zinegalelo ekuphuculeni kwiindawo ezahlukeneyo zesayensi, zoshishino, kunye nezonyango.
.