ke mofuta ofe oa ntho e ka aroloang ka ho sebelisa centrifuge

Lintho tse Arohanngoang ka ho Sebelisa Sentifuge: Matla a ho Arohanya ka ho Nepahala

Selelekela sa Centrifugation le Litšebeliso tsa Eona

Ho sebelisa centrifugation ke mokhoa o sebelisoang haholo lits'ebetsong tse fapaneng tsa mahlale le tsa indasteri ho arola lintho ho latela bongata le boholo ba tsona. Mokhoa ona o matla o itšetlehile ka ts'ebeliso ea matla a centrifugal, a hlahisoang ke ho potoloha sampole ka lebelo le phahameng, ho fihlella karohano e sebetsang hantle. Ho tloha lisampoleng tsa baeloji lilaboratoring ho isa tlhahisong ea lihlahisoa tse fapaneng indastering, li-centrifuge li fumana lits'ebetso masimong a fapaneng. Sehloohong sena, re tla hlahloba mefuta ea lintho tse ka aroloang ho sebelisoa centrifuge le bohlokoa ba tsona mafapheng a fapaneng.

Ho Arola Lisele le Likaroloana

E 'ngoe ea lits'ebetso tsa mantlha tsa ho centrifugation ke karohanong ea lisele le likaroloana. Ka ho sebelisa liphapang tsa boholo le bongata, centrifugation e lumella ho aroloa ha likarolo tse itseng ho tsoa lisampoleng tse rarahaneng. Mohlala, lilaboratoring tsa bongaka, centrifuge li sebelisoa haholo ho arola le ho ithuta lisele tsa mali. Ha sampole ea mali e sothoa ka centrifuge ka lebelo le phahameng, lisele tse khubelu tsa mali tse boima li lula tlase ha plasma e bobebe e lula holimo, e leng se thusang tlhahlobo e eketsehileng.

Ka ho tšoanang, lipatlisisong tsa microbiology, ho centrifugation ho phetha karolo ea bohlokoa ho fumaneng litso tse hloekileng tsa baktheria le tomoso. Ka ho laola ka hloko lebelo le nako ea ho centrifugation, likokoana-hloko tsena li ka aroloa ho tsoa mecheng e potolohileng, ho thusa ho ithuta litšobotsi tsa tsona, metabolism le mekhoa ea kholo.

Karohano e Nepahetseng ea DNA le RNA

Ho thiba lisele tsa lefutso ho bohlokoa haholo ho aroleng li-nucleic acid, tse kang DNA le RNA, lisampoleng tsa baeloji. Lisebelisoa tsena tsa lefutso hangata li hloka ho ntšoa ka mokhoa oa tsona o hloekileng ka ho fetisisa bakeng sa lits'ebetso tse fapaneng, ho kenyeletsoa lipatlisiso tsa lefutso le merero ea tlhahlobo. Mekhoa ea ho thiba lisele tsa lefutso, joalo ka ho thiba lisele tsa lefutso tsa density gradient, e lumella karohano ea li-nucleic acid ho tsoa likarolong tse ling tsa sele, joalo ka liprotheine le lipids.

Ts'ebetso ena e kenyelletsa ho kenya sampole ea baeloji holim'a sesebelisoa sa ho lekanya bongata, hangata tharollo ea sucrose kapa cesium chloride, le ho e centrifuga ka lebelo le phahameng. Nakong ea ho centrifugation, li-nucleic acid lia falla ka har'a tekanyo ea bongata ho fihlela li fihla sebakeng se nang le bongata bo itseng bo lumellanang le ba bona. Mokhoa ona oa karohano e nepahetseng o nolofalletsa ho ntšoa ha DNA e hloekileng le RNA bakeng sa lits'ebetso tse tlase joalo ka PCR, tatellano ea liphatsa tsa lefutso, le tlhahlobo ea polelo ea liphatsa tsa lefutso.

Ho Hloekisa Limolek'hule

Ho centrifugation ho bohlokoa bakeng sa ho hlwekisa di-biomolecule tse fapaneng, ho kenyeletsoa le diprotheine le di-enzyme. Dilaboratoring tsa biochemistry le biotechnology, mefuta e fapaneng ya di-centrifuge e sebediswa ho arola le ho hlwekisa dimolekhule tsena tse rarahaneng. Ka ho sebedisa thepa ya tsona e ikgethang, jwalo ka boholo, sebopeho le bongata, mekgwa ya ho centrifugation e tlosa ditshila ka katleho mme e tsepamisa mohopolo wa biomolecule e lakatsehang.

Ultracentrifugation, e leng mokhoa o matla o sebelisang lebelo le phahameng haholo, hangata o sebelisoa bakeng sa ho hloekisa le ho khetholla liprotheine. Ts'ebetsong ena, lisampole li pepesetsoa matla a matla a fetang matla a khoheli a Lefatše ka makhetlo a likete, e leng se lebisang karohanong e nepahetseng ea likarolo tse fapaneng tsa protheine. Sena se nolofalletsa bafuputsi ho arola liprotheine tse itseng kapa metsoako ea liprotheine tse khahlisang, ho thusa lithuto tse tebileng tsa meaho le mesebetsi ea tsona ea limolek'hule.

Litšebeliso tsa Indasteri tsa Centrifugation

Ka ntle ho dipatlisiso tsa mahlale, ho thiba dikotwana ho bapala karolo ya bohlokwa ditsamaisong tse ngata tsa indasteri. Indastering ya dijo le dino, di-centrifuge di sebediswa ho arola dintho tse tiileng ho diedi, ho kopanya lero la ditholwana, ho hlakisa veine, le ho hlakisa oli, hara ditshebediso tse ding. Ka ho tlosa ditshila, tse kang dintho tse tiileng, dikarolwana tse leketlileng, kapa disele tse sa batleheng tsa dikokwana-hloko, di-centrifuge di thusa ho ntlafatsa boleng ba sehlahiswa le ho eketsa chai.

Indasteri ea meriana e boetse e itšetlehile haholo ka ho centrifugation bakeng sa mekhoa ea karohano le tlhoekiso nakong ea tlhahiso ea meriana le liente. Liente li sebelisoa ho ntšeng metsoako e sebetsang limela, ho kotula lijalo tsa sele, ho hloekisa protheine le tlhahiso ea liente.

Qetello:

Ho centrifugation ke mokhoa o feto-fetohang o fanang ka karohano e nepahetseng ea lintho tse fapaneng ho latela bongata le boholo ba tsona. Ho tloha ho arola likarolo tsa mali lilaboratoring tsa bongaka ho isa ho arola liprotheine bakeng sa lipatlisiso le ho hloekisa metsoako ea meriana, ho centrifuge ke lisebelisoa tsa bohlokoa haholo lits'ebetsong tse fapaneng. Ha theknoloji e ntse e tsoela pele, mekhoa ea ho centrifugation e ntse e tsoela pele ho fetoha le ho kenya letsoho tsoelo-peleng mafapheng a mangata a saense, indasteri le bongaka.