he aha te momo matū ka taea te wehe mā te whakamahi i te centrifuge
Ngā Matū Wehewehea mā te Whakamahi i te Centrifuge: He Pūngao mō te Wehewehenga Tika
He Kupu Whakataki ki te Whakapūtanga me ōna Whakamahinga
Ko te centrifugation he tikanga e whakamahia whānuitia ana i roto i ngā tukanga pūtaiao me ngā tukanga ahumahi hei wehewehe i ngā matū i runga i tō rātou kiato me te rahi. Ko tēnei tikanga kaha e whakawhirinaki ana ki te whakamahinga o te kaha centrifugation, i hangaia mā te hurihuri i te tauira i ngā tere teitei, kia tutuki ai te wehenga whai hua. Mai i ngā tauira koiora i roto i ngā taiwhanga ki te whakaputa ahumahi o ngā hua rerekē, ka kitea ngā tono a ngā centrifugation i roto i te whānuitanga o ngā mara. I roto i tēnei tuhinga, ka tūhuratia e mātou ngā momo matū ka taea te wehewehe mā te whakamahi i te centrifuge me tō rātou hiranga i roto i ngā rāngai rerekē.
Te Wehewehe i ngā Pūtau me ngā Matūriki
Ko tētahi o ngā whakamahinga matua o te centrifugation ko te wehenga o ngā pūtau me ngā matūriki. Mā te whakamahi i ngā rerekētanga o te rahi me te mātotoru, ka taea e te centrifugation te wehe i ngā wāhanga motuhake mai i ngā tauira uaua. Hei tauira, i roto i ngā taiwhanga hauora, he nui te whakamahinga o ngā centrifugation hei wehe me te ako i ngā pūtau toto. Ina hurihia he tauira toto i roto i te centrifuge i ngā tere teitei, ka tau ngā pūtau toto whero taumaha ki raro, ko te plasma māmā ake ka noho ki runga, ka āwhina i te tātaritanga atu.
Waihoki, i roto i te rangahau moroiti, he mea nui te mahi a te centrifugation ki te whiwhi i ngā ahurea parakore o ngā huakita me ngā rewena. Mā te āta whakahaere i te tere me te roa o te centrifugation, ka taea te wehe i ēnei moroiti mai i ngā mea e karapoti ana, hei āwhina i te ako i ō rātou āhuatanga, te pākia o te tinana, me ngā tauira tipu.
Te Wehenga Tika o te DNA me te RNA
He mea nui te whakamahi i te pūwero hei wehe i ngā waikawa karihi, pērā i te DNA me te RNA, mai i ngā tauira koiora. He maha ngā wā me tango mai ēnei rauemi ira i roto i tō rātou āhua parakore mō ngā momo mahi, tae atu ki te rangahau ira me ngā kaupapa tātaritanga. Mā ngā tikanga pūwero, pērā i te pūwero whakarara matotoru, ka taea te wehe i ngā waikawa karihi mai i ētahi atu wāhanga pūtau, pērā i ngā pūmua me ngā lipid.
Ko te tukanga he uta i te tauira koiora ki runga i tētahi reo pikinga mātotoru, he otinga sucrose, he cesium chloride rānei, ā, he tere te whakateretere. I te wā e whakatereteretia ana, ka heke ngā waikawa karihi mā roto i te pikinga mātotoru kia tae rā anō ki tētahi rohe he mātotoru motuhake e ōrite ana ki tō rātou ake. Mā tēnei tikanga wehewehe tika ka taea te tango i te DNA me te RNA parakore mō ngā tono o muri mai pērā i te PCR, te raupapatanga ira, me te tātari whakaaturanga ira.
Te Whakamaemae i ngā Ira Koiora
He mea nui te whakamahi i te pūwero hei pure i ngā momo ngota koiora, tae atu ki ngā pūmua me ngā whākōkī. I roto i ngā taiwhanga matū koiora me te hangarau koiora, ka whakamahia ngā momo pūwero rerekē hei wehe me te pure i ēnei ngota matatini. Mā te whakamahi i ō rātou āhuatanga motuhake, pērā i te rahi, te āhua, me te mātotoru, ka tangohia e ngā tikanga pūwero ngā poke me te whakatōtika i te ngota koiora e hiahiatia ana.
Ko te ultracentrifugation, he tikanga kaha e whakamahi ana i ngā tere tino teitei, e whakamahia ana mō te purenga me te whakaahua i ngā pūmua. I roto i tēnei tukanga, ka tukuna ngā tauira ki ngā kaha e mano ngā wā kaha ake i te kaha ā-papa o te Ao, ka hua ake he wehenga tika o ngā wāhanga pūmua rerekē. Mā tēnei ka taea e ngā kairangahau te wehewehe i ngā pūmua motuhake, i ngā matatini pūmua rānei e hiahiatia ana, ka āwhina i ngā rangahau hōhonu mō ō rātou hanganga me ngā mahi ngota.
Ngā Whakamahinga Ahumahi o te Whakapūtanga
Haunga te rangahau pūtaiao, he mea nui te mahi a te centrifugation i roto i ngā tukanga ahumahi maha. I roto i te umanga kai me te inu, ka whakamahia ngā centrifugation hei wehewehe i ngā mea totoka mai i ngā wai, hei whakatōtō i ngā wai hua, hei whakamārama i te waina, hei whakamārama i ngā hinu, me ētahi atu whakamahinga. Mā te tango i ngā poke, pērā i ngā mea totoka, ngā matūriki iri, ngā pūtau moroiti kāore e hiahiatia, ka āwhina ngā centrifugation ki te whakarei ake i te kounga o te hua me te whakapiki ake i te hua.
He nui hoki te whakawhirinaki o te umanga rongoā ki te centrifugation hei wehewehe me te purenga i te wā e hangaia ana ngā rongoā me ngā kano kano. E whakamahia ana ngā centrifuges hei tango i ngā matū hohe mai i ngā tipu, hei hauhake i ngā ahurea pūtau, hei purenga pūmua, me te hanga kano kano.
Whakatau:
He tikanga maha ngā mahi a te centrifugation e whakarato ana i te wehenga tika o ngā matū rerekē i runga i tō rātou kiato me te rahi. Mai i te wehewehe i ngā wāhanga toto i roto i ngā taiwhanga hauora ki te wehe i ngā pūmua mō te rangahau me te purenga o ngā matū rongoā, he taputapu tino nui ngā centrifugation i roto i te whānuitanga o ngā tono. I te whanaketanga o te hangarau, ka haere tonu ngā tikanga centrifugation ki te whanake me te whai wāhi ki ngā whanaketanga puta noa i ngā mara pūtaiao, ahumahi, me te hauora maha.
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