rudzii rwechinhu chinogona kupatsanurwa uchishandisa centrifuge

Zvinhu Zvinoparadzaniswa Uchishandisa Centrifuge: Simba Rekuparadzanisa Kwakarurama

Nhanganyaya yeCentrifugation uye Mashandisirwo Ayo

Kugadzira centrifugation inzira inoshandiswa zvakanyanya mumabasa akasiyana-siyana esainzi nemaindasitiri kupatsanura zvinhu zvichienderana nehuremu hwazvo nehukuru hwazvo. Nzira iyi ine simba inotsamira pakushandiswa kwesimba re centrifugal, rinogadzirwa nekutenderedza sampuli nekumhanya kwakanyanya, kuti riwane kupatsanurwa kwakanaka. Kubva kusampuli dzezvipenyu mumarabhoritari kusvika pakugadzirwa kwezvigadzirwa zvakasiyana-siyana mumaindasitiri, ma centrifuge anowana mashandisirwo muminda yakasiyana-siyana. Muchinyorwa chino, tichaongorora mhando dzezvinhu zvinogona kupatsanurwa uchishandisa centrifuge uye kukosha kwazvo muzvikamu zvakasiyana.

Kuparadzanisa Masero neZvidimbu

Imwe yenzira huru dzekushandisa centrifugation ndeyekupatsanura masero nezvidimbu. Nekushandisa musiyano wehukuru nehuremu, centrifugation inobvumira kupatsanurwa kwezvikamu zvakati kubva musampuli dzakaoma. Semuenzaniso, mumarabhoritari ekurapa, centrifuges dzinoshandiswa zvakanyanya kupatsanura nekudzidza masero eropa. Kana sampuro yeropa ikatenderedzwa mucentrifuge nekumhanya kwakanyanya, masero matsvuku eropa akaremara anogara pasi nepo plasma yakareruka inoramba iri pamusoro, zvichiita kuti kuongororwa kuwedzere.

Saizvozvowo, mukutsvagurudza nezve microbiology, centrifugation inoita basa rakakosha mukuwana mhando dzakachena dzemabhakitiriya nembiriso. Nekunyatso dzora kumhanya uye nguva ye centrifugation, tupukanana utwu tunogona kuparadzaniswa kubva kune zvakatikomberedza, zvichibatsira mukudzidza hunhu hwatwo, metabolism, uye maitiro ekukura.

Kupatsanurwa Kwakarurama kweDNA neRNA

Kubatanidza ma "centrifugation" kwakakosha pakuparadzanisa ma "nucleic acids", akadai seDNA neRNA, kubva mu "biological samples". Izvi zvinhu zvema "genetic" zvinowanzoda kutorwa muchimiro chazvo chakachena kuti zvishandiswe zvakasiyana-siyana, kusanganisira kutsvagisa majini uye zvinangwa zvekuongorora. Maitiro ekubatanidza ma "centrifugation", akadai se "density gradient centrifugation", anobvumira kupatsanurwa kwe "nucleic acids" kubva kune zvimwe zvikamu zvemasero, zvakaita semapuroteni nemafuta.

Maitiro aya anosanganisira kurodha sample yebiological pane density gradient medium, inowanzova sucrose kana cesium chloride solution, uye kuitenderedza uchishandisa high speed. Munguva ye centrifugation, nucleic acids inofamba nepakati pe density gradient kusvika yasvika kunzvimbo ine density chaiyo inoenderana neyayo. Iyi nzira yekuparadzanisa nemazvo inogonesa kuburitsa DNA yakachena neRNA yekushandisa pasi senge PCR, genetic sequencing, uye gene expression analysis.

Kuchenesa Biomolecules

Kubatanidza zvinhu zvakasiyana-siyana (centrifugation) kwakakosha pakuchenesa zvinhu zvakasiyana-siyana zvema "biomolecules", kusanganisira mapuroteni nema "enzymes". Mumarabhoritari e biochemistry ne biotechnology, mhando dzakasiyana dze "centrifuges" dzinoshandiswa kuparadzanisa nekuchenesa mamorekuru aya akaomarara. Nekushandisa hunhu hwawo hwakasiyana, hwakadai sehukuru, chimiro, uye kuwanda, matekiniki ekubatanidza zvinhu zvakasiyana-siyana anobvisa tsvina uye anosimbisa zvinhu zvinodiwa.

Ultracentrifugation, nzira ine simba inoshandisa kumhanya kwakanyanya, inowanzoshandiswa pakuchenesa nekutsanangura mapuroteni. Mukuita uku, sampuro dzinosangana nemasimba akasimba zvakapetwa zviuru zvakawanda kupfuura simba repasi, zvichiita kuti zvikamu zvakasiyana zvemapuroteni zviparadzaniswe nemazvo. Izvi zvinogonesa vaongorori kupatsanura mapuroteni chaiwo kana mapuroteni anodiwa, zvichiita kuti pave nekudzidza kwakadzama kwemaumbirwo nemashandiro awo emamorekuru.

Mashandisirwo eCentrifugation muMaindasitiri

Kupfuura tsvakurudzo yesainzi, centrifuge inoita basa rakakosha mumaindasitiri akawanda. Muindasitiri yezvikafu nezvinwiwa, centrifuge dzinoshandiswa kuparadzanisa zvinhu zvakasimba kubva muzvinwiwa, kusanganisa muto wemichero, kujekesa waini, uye kujekesa mafuta, pakati pezvimwe zvinoshandiswa. Nekubvisa tsvina, senge zvinhu zvakaoma, zvidimbu zvakamiswa, kana masero ehutachiona asingadiwe, centrifuge dzinobatsira kusimudzira kunaka kwechigadzirwa uye kuwedzera goho.

Indasitiri yemishonga inovimbawo zvakanyanya ne centrifugation yekuparadzanisa nekuchenesa panguva yekugadzira mishonga nemajekiseni. Centrifuges dzinoshandiswa mukubvisa zvinhu zvinoshanda kubva mumiti, kuunganidza masero, kuchenesa mapuroteni, uye kugadzira majekiseni.

Mhedziso:

Kupinza zvinhu mumasero (centrifugation) inzira inoshanda zvakasiyana-siyana inopa kupatsanurwa kwakanyatsojeka kwezvinhu zvakasiyana-siyana zvichienderana nehuwandu hwazvo nehukuru hwazvo. Kubva pakupatsanura zvikamu zveropa mumarabhoritari ekurapa kusvika pakupatsanura mapuroteni ekutsvaga nekuchenesa mishonga, macentrifuge zvishandiso zvakakosha mukushandiswa kwakasiyana-siyana. Sezvo tekinoroji ichifambira mberi, nzira dzekubatanidza zvinhu mumasero dziri kuramba dzichichinja uye dzichibatsira mukufambira mberi mune dzakawanda nzvimbo dzesainzi, maindasitiri, uye zvokurapa.