hore na likarolo tse peli tsa mali li aroloa nakong ea centrifugation

Selelekela

Mali ke karolo ea bohlokoa ea 'mele oa motho, a jara oksijene, limatlafatsi, lihormone le litšila ho ea lithong le liseleng tse fapaneng. A na le plasma, lisele tse khubelu tsa mali (RBC), lisele tse tšoeu tsa mali (WBC), le li-platelet. Leha ho le joalo, ka ts'ebetso e bitsoang centrifugation, mali a ka aroloa ka likarolo tse fapaneng. Sengoloa sena se hlahloba likarolo tse peli tse ka sehloohong tsa mali tse aroloang nakong ea centrifugation ho utloisisa mesebetsi ea tsona, bohlokoa le ts'ebeliso e ka bang teng.

Ho utloisisa Centrifugation

Ho centrifugation ke mokhoa o sebelisang matla a centrifugal ho arola likarolo tse fapaneng tsa sampole ea mokelikeli ho latela bongata ba tsona. Tabeng ea mali, ho centrifugation ho sebelisoa ho arola likarolo tsa sele ho tsoa ho karolo ea mokelikeli (plasma). Karohano ena e ka etsahala hobane likarolo tse fapaneng tsa mali li na le bongata bo fapaneng.

Plasma: Khauta e Metsi

Plasma e etsa karolo ea mokelikeli maling 'me e etsa hoo e ka bang 55% ea bophahamo bohle ba eona. Ke mokelikeli o mosehla o nang le metsi, li-electrolyte, liprotheine, lihormone, lienzyme le lihlahisoa tsa litšila. Plasma e sebetsa e le sesebelisoa sa bohlokoa sa ho tsamaisa, e jara lintho tsa bohlokoa tse kang limatlafatsi, lihormone le lihlahisoa tsa litšila ho pholletsa le 'mele. E boetse e bapala karolo ea bohlokoa ho bolokeng botsitso ba 'mele ba acid-base le ho laola khatello ea mali.

Lisele tse Khubelu tsa Mali: Lijari tsa Oksijene

Lisele tse khubelu tsa mali, tse tsejoang hape e le li-erythrocyte, ke karolo e ngata ka ho fetisisa ea lisele tsa mali, e etsang hoo e ka bang 45% ea boholo ba eona kaofela. Li ikarabella bakeng sa ho tsamaisa oksijene ho tloha matšoafong ho ea liseleng le lithong tse fapaneng le ho khutlisetsa carbon dioxide matšoafong bakeng sa ho tlosoa. Bokhoni bona bo tsotehang ba ho jara oksijene bo bakoa haholo-holo ke boteng ba protheine e bitsoang hemoglobin ka har'a li-RBC. Hemoglobin e tlama oksijene matšoafong 'me e e ntša liseleng moo tlhoko ea oksijene e leng holimo.

Lisele tse Tšoeu tsa Mali: Balebeli ba Tsamaiso ea 'Mele ea ho Itšireletsa

Lisele tse tšoeu tsa mali, kapa li-leukocyte, li phetha karolo ea bohlokoa ho sireletseng 'mele khahlanong le tšoaetso le mafu. Le hoja li etsa karolo e nyane feela ea bongata bohle ba mali (ka tlase ho 1%), bohlokoa ba tsona bo ke ke ba fokotsoa. ​​Li-WBC li fapane haholo, li entsoe ka mefuta e fapaneng ea lisele tse kang li-neutrophils, li-lymphocyte, li-monocyte, li-eosinophils le li-basophils. Mofuta o mong le o mong o na le mesebetsi e itseng e amanang le tšireletso ea 'mele, joalo ka ho loantša libaktheria, livaerase, likokoana-hloko le lintho tse ling tse tsoang linaheng tse ling tse kenang' meleng.

Litšebeliso tsa Kliniki tsa Centrifugation

Ho centrifugation ho sebelisoa haholo libakeng tsa bongaka bakeng sa merero ea tlhahlobo le kalafo. Ka ho arola mali ka likarolo tsa ona, litsebi tsa tlhokomelo ea bophelo li ka hlahloba likarolo tse fapaneng ho fumana bophelo bo botle ka kakaretso le ho fumana maemo a fapaneng. Mohlala, ho sekaseka plasma ho ka fana ka tlhaiso-leseling mabapi le ts'ebetso ea liphio le sebete, liprofaele tsa lipid, maemo a lihormone le tse ling. Ho hlahloba likarolo tsa sele, haholo-holo WBC, ho ka fana ka temohisiso ea bohlokoa mabapi le boteng ba tšoaetso kapa lintho tse sa tloaelehang tsamaisong ea 'mele ea ho itšireletsa mafung.

Monehelo oa Likarolo tsa Mali

Karohano ea likarolo tsa mali ka ho centrifugation ke ea bohlokoa litsing tsa monehelo oa mali. Mali a felletseng hangata a bokelloa ho bafani, ebe a aroloa ka likarolo tsa ona ho eketsa tšebeliso ea ona. Ka ho arola li-RBC, plasma le li-platelet, karolo ka 'ngoe e ka sebeletsa litlhoko tse fapaneng tsa bongaka. Mohlala, li-RBC li sebelisoa khafetsa ho phekola khaello ea mali, ha plasma e sebelisoa bakeng sa tšelo ea mali, tlhahiso ea lintho tse hoamang mali, le tsamaiso ea albumin. Li-platelet lia hlokahala bakeng sa bakuli ba nang le mathata a ho tsoa mali kapa ba fuoang chemotherapy.

Bokamoso ba Karohano ea Likarolo tsa Mali

Tsoelo-pele e tsoelang pele theknolojing ea centrifugation e ntlafatsa bokhoni le ho atoloha ha karohano ea likarolo tsa mali. Bafuputsi ba ntse ba hlahloba mekhoa e mecha ea ho ntlafatsa ts'ebetso ea karohano haholoanyane, ho fumana likarolo tsa boleng bo holimo, le ho nts'etsapele lits'ebetso tse ncha. Tsoelo-pele ena e na le litlamorao tse kholo masimong a meriana ea tsosoloso, ho sibolloa ha lithethefatsi le tlhokomelo ea bophelo bo botle ea motho ka mong, moo likarolo tsa mali tse hloekisitsoeng li ka sebelisoang bakeng sa mekhoa ea kalafo.

Qetellong, ho arola mali ka sekontiri ke mokhoa oa bohlokoa oa ho arola likarolo tsa mali. Ka ho arola mali ka likarolo tsa plasma le lisele, tse kang lisele tse khubelu le tse tšoeu tsa mali, litsebi tsa tlhokomelo ea bophelo li ka hlahloba likarolo tse fapaneng, tsa etsa tlhahlobo, le ho phekola maemo a fapaneng ka katleho. Karohano ea likarolo tsa mali e nolofalitse tsoelo-pele ea bongaka 'me e ntse e le mokhoa oa bohlokoa libakeng tsa bongaka, litsi tsa monehelo oa mali le lilaboratori tsa lipatlisiso lefatšeng ka bophara.