ob yam khoom ntawm cov ntshav uas raug sib cais thaum lub sijhawm centrifugation

Kev Taw Qhia

Ntshav yog ib feem tseem ceeb ntawm tib neeg lub cev, nqa cov pa oxygen, cov as-ham, cov tshuaj hormones, thiab cov khoom pov tseg mus rau ntau yam kab mob thiab cov nqaij. Nws muaj cov plasma, cov qe ntshav liab (RBCs), cov qe ntshav dawb (WBCs), thiab cov platelets. Txawm li cas los xij, los ntawm cov txheej txheem hu ua centrifugation, ntshav tuaj yeem raug cais ua cov khoom sib txawv. Tsab xov xwm no tshawb nrhiav ob qho tseem ceeb ntawm cov ntshav uas tau sib cais thaum lub sijhawm centrifugation kom nkag siab txog lawv cov haujlwm, qhov tseem ceeb, thiab cov kev siv tau.

Nkag Siab Txog Kev Siv Centrifugation

Kev centrifugation yog ib qho txheej txheem uas siv lub zog centrifugal los cais cov khoom sib txawv ntawm cov kua dej raws li lawv qhov ceev. Hauv cov ntshav, centrifugation yog siv los cais cov khoom hauv lub cellular ntawm cov kua (plasma). Qhov kev sib cais no ua tau vim tias cov khoom sib txawv ntawm cov ntshav muaj qhov ceev sib txawv.

Plasma: Cov Kub Ua Kua

Cov ntshav (plasma) yog cov kua dej uas muaj nyob hauv cov ntshav thiab suav txog li 55% ntawm nws tag nrho cov ntim. Nws yog cov kua daj uas muaj dej, electrolytes, proteins, hormones, enzymes, thiab cov khoom pov tseg. Plasma ua haujlwm ua ib qho chaw thauj mus los tseem ceeb, nqa cov khoom tseem ceeb xws li cov as-ham, hormones, thiab cov khoom pov tseg thoob plaws lub cev. Nws kuj tseem ua lub luag haujlwm tseem ceeb hauv kev tswj lub cev cov kua qaub-puag thiab tswj cov ntshav siab.

Cov Qog Ntshav Liab: Cov Neeg Nqa Pa oxygen

Cov qe ntshav liab, tseem hu ua erythrocytes, yog cov feem ntau ntawm cov ntshav, suav txog li 45% ntawm nws tag nrho. Lawv yog lub luag haujlwm thauj cov pa oxygen los ntawm lub ntsws mus rau ntau yam nqaij thiab cov kabmob thiab coj cov pa roj carbon dioxide rov qab mus rau lub ntsws kom tshem tawm. Lub peev xwm nqa cov pa oxygen zoo kawg no yog vim muaj cov protein hu ua hemoglobin hauv RBCs. Hemoglobin khi rau cov pa oxygen hauv lub ntsws thiab tso nws tawm hauv cov nqaij uas xav tau oxygen ntau.

Cov Qog Ntshav Dawb: Cov Neeg Saib Xyuas Lub Cev Tiv Thaiv Kab Mob

Cov qe ntshav dawb, lossis leukocytes, ua lub luag haujlwm tseem ceeb hauv kev tiv thaiv lub cev tiv thaiv kev kis kab mob thiab kab mob. Txawm hais tias lawv tsuas yog ib feem me me ntawm tag nrho cov ntshav (tsawg dua 1%), lawv qhov tseem ceeb tsis tuaj yeem raug rhuav tshem. WBCs muaj ntau yam sib txawv, muaj ntau hom subtypes xws li neutrophils, lymphocytes, monocytes, eosinophils, thiab basophils. Txhua hom subtype muaj cov haujlwm tshwj xeeb ntsig txog kev tiv thaiv kab mob, xws li tawm tsam cov kab mob, cov kab mob, cov kab mob parasite, thiab lwm yam khoom txawv teb chaws uas nkag mus rau hauv lub cev.

Cov Kev Siv Hauv Tsev Kho Mob ntawm Centrifugation

Kev siv centrifugation dav dav hauv kev kho mob rau kev kuaj mob thiab kev kho mob. Los ntawm kev faib cov ntshav ua nws cov khoom, cov kws kho mob tuaj yeem soj ntsuam ntau yam kev ntsuas los txiav txim siab txog kev noj qab haus huv tag nrho thiab kuaj mob ntau yam mob. Piv txwv li, kev tshuaj xyuas cov ntshav plasma tuaj yeem muab cov ntaub ntawv hais txog kev ua haujlwm ntawm lub raum thiab lub siab, cov qauv lipid, cov tshuaj hormones, thiab ntau ntxiv. Kev tshuaj xyuas cov khoom ntawm lub cev, tshwj xeeb tshaj yog WBCs, tuaj yeem muab cov kev nkag siab zoo rau qhov muaj kev kis kab mob lossis kev tsis zoo hauv lub cev tiv thaiv kab mob.

Kev Pub Ntshav

Kev sib cais cov ntshav los ntawm kev siv lub tshuab centrifugation yog qhov tseem ceeb hauv cov chaw pub ntshav. Cov ntshav tag nrho feem ntau raug sau los ntawm cov neeg pub ntshav, uas tom qab ntawd raug cais ua nws cov khoom kom siv tau zoo tshaj plaws. Los ntawm kev sib cais RBCs, plasma, thiab platelets, txhua yam khoom tuaj yeem pab tau rau ntau yam kev xav tau kev kho mob. Piv txwv li, RBCs feem ntau siv rau kev kho mob anemia, thaum plasma siv rau kev hloov ntshav, kev tsim cov khoom ntshav txhaws, thiab kev tswj hwm albumin. Platelets yog qhov tseem ceeb rau cov neeg mob uas muaj teeb meem ntshav lossis tab tom kho mob chemotherapy.

Yav Tom Ntej ntawm Kev Sib Cais Cov Cheebtsam Ntshav

Kev nce qib tas mus li hauv kev siv tshuab centrifugation ua rau kev sib cais cov ntshav ua haujlwm tau zoo dua thiab muaj peev xwm nthuav dav. Cov kws tshawb fawb tab tom tshawb nrhiav cov tswv yim tshiab los txhim kho cov txheej txheem sib cais, tau txais cov khoom zoo, thiab muaj peev xwm tsim cov ntawv thov tshiab. Cov kev nce qib no muaj feem cuam tshuam loj heev hauv kev kho mob rov qab, kev tshawb nrhiav tshuaj, thiab kev kho mob tus kheej, qhov twg cov khoom ntshav huv tuaj yeem siv rau kev kho mob.

Xaus lus, centrifugation yog ib qho txheej txheem tseem ceeb rau kev cais cov khoom ntawm cov ntshav. Los ntawm kev faib cov ntshav ua cov plasma thiab cov khoom ntawm tes, xws li cov qe ntshav liab thiab cov qe ntshav dawb, cov kws kho mob tuaj yeem soj ntsuam cov yam ntxwv sib txawv, kuaj mob, thiab kho ntau yam mob kom zoo. Kev cais cov khoom ntawm cov ntshav tau ua rau muaj kev nce qib hauv kev kho mob thiab tseem yog ib txoj hauv kev tseem ceeb hauv kev kho mob, chaw pub ntshav, thiab chaw soj ntsuam thoob ntiaj teb.