motheo oa ho arola metsoako ka ho sebelisa centrifuge ke ofe?

Ho Centrifugation: Ho Senola Saense e ka Morao ho Metswako e Arohanyang

Selelekela:

Metswako e fumaneha hohle bophelong ba rona ba letsatsi le letsatsi, ho tloha linthong tseo re kopanang le tsona ho isa linthong tseo re li jang. Liindastering tse fapaneng, karohano le tlhoekiso ea metsoako ena ke tsa bohlokoa bakeng sa tlhahiso ea lihlahisoa tsa boleng bo holimo. Mokhoa o mong o sebetsang hantle o sebelisoang bakeng sa morero ona ke ho kopanya metsoako ka sekontiri. Sengoloa sena se tla hlahloba melao-motheo le mekhoa ea ho kopanya metsoako ka sekontiri, se hlahlobe hore na e sebelisoa joang ho arola metsoako ka har'a lits'ebetso tse fapaneng.

Saense e Bakang ho Centrifugation

Ho centrifugation, ka mokhoa o bonolo ka ho fetisisa, ho kenyelletsa tšebeliso ea setshelo se potolohang ka potlako ho arola lintho tse nang le bongata bo fapaneng. Matla a centrifugal a hlahisoang nakong ea ho potoloha a etsa hore likarolo tse teteaneng li fallele ka ntle, li li arole ho metsoako e bobebe. Molao-motheo ona oa motheo o sebelisoa ho fihlela karohano liindastering tse fapaneng, ho tloha meriana ho ea kalafong ea metsi a litšila.

Mefuta le Likarolo tsa Li-Centrifuge

Li-centrifuge li tla ka mefuta e fapaneng ea meralo, e 'ngoe le e 'ngoe e etselitsoe litlhoko tse itseng. Mefuta e meraro ea mantlha ke - li-centrifuge tse lebelo le tlase, tse lebelo le phahameng, le tse lebelo le phahameng. Li-centrifuge tse lebelo le tlase li sebetsa ka lebelo le fihlang ho li-revolutions tse 6,000 ka motsotso (rpm) 'me li sebelisoa hangata lilaboratoring tsa bongaka bakeng sa lits'ebetso tse tloaelehileng. Li-centrifuge tse lebelo le phahameng, ka lehlakoreng le leng, li ka fihla lebelong le fihlang ho 20,000 rpm 'me li sebelisoa lilaboratoring tsa lipatlisiso bakeng sa lits'ebetso tse rarahaneng tsa karohano. Li-centrifuge tse lebelo le phahameng ke tsona tse matla ka ho fetisisa, tse fihlang lebelong le makatsang la ho feta 100,000 rpm, tse lumellang karohano ea esita le likaroloana tse ntle ka ho fetisisa.

Ho sa tsotelehe mofuta oa eona, hangata li-centrifuge li na le likarolo tse tharo tsa bohlokoa: rotor, enjene le sistimi ea taolo. Rotor e tšoara lipeipi tsa sampole 'me e potoloha ka lebelo le phahameng, e hlahisa matla a centrifugal. Enjene e potlakisa le ho fokotsa lebelo la rotor, ha sistimi ea taolo e nolofalletsa liphetoho tsa nako, lebelo le maemo a mocheso.

Ho Centrifugation Lipatlisisong tsa Bongaka

Phuputsong ea bongaka, ho centrifugation ho phetha karolo ea bohlokoa ho aroleng likarolo tsa mali le ho bokella limolek'hule tsa bohlokoa. Tšebeliso e 'ngoe e hlahelletseng ke ho ntšoa ha plasma kapa serum maling 'ohle. Kamora ho bokelloa, mali a kenngoa ka har'a centrifuge 'me a phethoa ka lebelo le nako e itseng. Lisele tse khubelu tsa mali tse teteaneng li lula tlase, ha plasma e sa teteaneng haholo e bokellana ka holimo. Karohano ena e lumella bo-rasaense ho sekaseka likarolo tse fapaneng, tse kang liprotheine, li-enzyme, lihormone le lintho tsa lefutso, ho thusa ho hlahloba, nts'etsopele ea meriana le lipatlisiso tsa mafu.

Likopo tsa Liindasteri

Ho sebelisa centrifuge ho fumana sebaka sa eona makaleng a 'maloa a indasteri, ho khotsofatsa litlhoko tse ikhethang tsa e 'ngoe le e 'ngoe. Indastering ea lijo, li-centrifuge li sebelisoa ho arola lintho tse tiileng ho tse metsi, hammoho le ho hlakisa lero, oli le veine. Ka ho arola litšila tse sa batleheng, li-centrifuge tsena li ntlafatsa boleng le bohloeki ba lihlahisoa.

Ho feta moo, lifeme tsa kalafo ea metsi a litšila li sebelisa li-centrifuge ho arola mekhahlelo e tiileng le ea metsi nakong ea lits'ebetso tsa ho hlakisa. Li-centrifuge li tlosa lintho tse tiileng tse emisitsoeng, seretse le litšila ka katleho, li netefatsa hore metsi a bolokehile bakeng sa ho sebelisoa hape kapa ho ntšoa. Ka mokhoa o ts'oanang, ho centrifuge ho sebelisoa indastering ea merafo bakeng sa merero ea ho ntša metsi, ho fumana liminerale tsa bohlokoa ka katleho.

Tokiso le Tlhokomelo ea Li-Centrifuge

Ho netefatsa liphetho tse nepahetseng le tse tsitsitseng, ho lokisoa hantle le tlhokomelo e nepahetseng ea li-centrifuge kamehla ho bohlokoa. Taba ea pele, rotor e tlameha ho leka-lekana, ka lipeipi tsa sampole li hlophisitsoe ka ho lekana. Ho se leka-lekane ho ka lebisa ho thothomeleng, lerata, esita le tšenyo ho centrifuge. Ho feta moo, ho leka-lekanya le ho hloekisa li-rotor, likoahelo le kamore khafetsa hoa hlokahala ho boloka ts'ebetso e ntle le ho thibela tšilafalo.

Qetello:

Ho centrifugation ho sebetsa e le mokhoa oa motheo bakeng sa karohano ea metsoako ho pholletsa le liindasteri tse ngata. Ka ho sebelisa melao-motheo ea matla a centrifugal, lintho tse nang le bongata bo fapaneng li ka aroloa ka katleho, tsa notlolla bokhoni ba tsona ba 'nete. Ho tloha lipatlisisong tsa bongaka ho isa lits'ebetsong tsa indasteri, ho feto-fetoha ha ho centrifugation ho e etsa sesebelisoa sa bohlokoa bakeng sa ho fihlela lihlahisoa tsa boleng bo holimo le tse hloekileng. Ka kutloisiso e nepahetseng ea saense e ka morao ho mokhoa ona le tlhokomelo ea kamehla, bo-rasaense le liindasteri ba ka tsoela pele ho sutumelletsa meeli ea saense ea karohano le ho khanna boqapi.