Dab tsi yog lub hauv paus rau kev sib cais cov khoom sib xyaw siv lub centrifuge
Kev Siv Centrifugation: Tshawb Pom Qhov Kev Tshawb Fawb Tom Qab Kev Sib Cais Cov Khoom Sib Xyaws
Kev Taw Qhia:
Cov khoom sib xyaw muaj nyob txhua qhov chaw hauv peb lub neej txhua hnub, txij li cov ntaub ntawv peb ntsib mus rau cov khoom peb noj. Hauv ntau yam lag luam, kev sib cais thiab kev ntxuav cov khoom sib xyaw no yog qhov tseem ceeb rau kev tsim cov khoom zoo. Ib txoj hauv kev zoo siv rau lub hom phiaj no yog centrifugation. Tsab xov xwm no yuav nkag mus rau hauv cov ntsiab cai thiab cov txheej txheem ntawm centrifugation, tshawb nrhiav seb nws siv li cas los cais cov khoom sib xyaw thoob plaws ntau yam kev siv.
Kev Tshawb Fawb Tom Qab Centrifugation
Kev siv lub tshuab centrifugation, uas yog ib qho yooj yim tshaj plaws, yog siv lub thawv uas tig sai sai los cais cov tshuaj uas muaj qhov sib txawv. Lub zog centrifugal uas tsim thaum lub sijhawm tig ua rau cov khoom sib xyaw ua ke txav mus rau sab nraud, cais lawv ntawm cov khoom sib dua. Lub hauv paus ntsiab lus no yog siv los ua kom sib cais hauv ntau yam lag luam, txij li tshuaj mus rau kev kho dej khib nyiab.
Hom thiab Cheebtsam ntawm Centrifuges
Cov centrifuges muaj ntau hom qauv, txhua tus tsim raws li qhov xav tau tshwj xeeb. Peb hom tseem ceeb yog - qis-ceev, siab-ceev, thiab ultracentrifuges. Cov centrifuges qis-ceev ua haujlwm ntawm qhov ceev txog li 6,000 revolutions ib feeb (rpm) thiab feem ntau siv hauv cov chaw kuaj mob rau kev siv niaj hnub. Cov centrifuges siab-ceev, ntawm qhov tod tes, tuaj yeem ncav cuag qhov ceev txog li 20,000 rpm thiab siv hauv cov chaw tshawb fawb rau cov txheej txheem sib cais nyuaj dua. Ultracentrifuges yog cov muaj zog tshaj plaws, ncav cuag qhov ceev tshaj plaws ntawm ntau dua 100,000 rpm, tso cai rau kev sib cais txawm tias cov khoom me me zoo tshaj plaws.
Txawm yog hom twg los xij, feem ntau cov centrifuges muaj peb yam tseem ceeb: lub rotor, lub cev muaj zog, thiab lub kaw lus tswj. Lub rotor tuav cov raj kuaj thiab tig ntawm qhov ceev ceev, tsim lub zog centrifugal. Lub cev muaj zog ua kom lub rotor nrawm thiab qeeb, thaum lub kaw lus tswj hwm ua rau muaj kev hloov kho lub sijhawm, qhov ceev, thiab qhov kub.
Centrifugation hauv Kev Tshawb Fawb Kho Mob
Hauv kev tshawb fawb txog kev kho mob, kev siv centrifugation ua lub luag haujlwm tseem ceeb hauv kev sib cais cov khoom xyaw ntawm cov ntshav thiab sau cov biomolecules muaj nqis. Ib qho kev siv tseem ceeb yog kev rho cov plasma lossis serum ntawm cov ntshav tag nrho. Tom qab sau tau, cov ntshav raug muab tso rau hauv lub centrifuge thiab tig ntawm qhov ceev thiab lub sijhawm tshwj xeeb. Cov qe ntshav liab uas tuab dua nyob hauv qab, thaum cov plasma uas tsis tuab dua sib sau ua ke saum toj. Qhov kev sib cais no tso cai rau cov kws tshawb fawb los tshuaj xyuas ntau yam khoom xyaw, xws li cov protein, enzymes, cov tshuaj hormones, thiab cov khoom siv caj ces, pab txhawb kev kuaj mob, kev tsim tshuaj, thiab kev tshawb fawb txog kab mob.
Cov Ntawv Thov Kev Lag Luam
Kev siv centrifugation pom nws qhov chaw hauv ntau qhov kev lag luam, ua kom tau raws li qhov xav tau ntawm txhua tus. Hauv kev lag luam zaub mov, centrifuges siv rau kev cais cov khoom khov thiab cov kua, nrog rau kev ua kom cov kua txiv hmab txiv ntoo, roj, thiab cawv txiv hmab ntshiab. Los ntawm kev cais cov khoom tsis huv uas tsis xav tau, cov centrifuges no txhim kho qhov zoo thiab kev huv ntawm cov khoom.
Tsis tas li ntawd xwb, cov chaw kho dej khib nyiab siv cov centrifuges los cais cov khoom khov thiab cov kua thaum lub sijhawm ua kom huv. Cov centrifuges tshem tawm cov khoom khov, cov av qias neeg, thiab cov khoom tsis huv, ua kom cov dej muaj kev nyab xeeb rau kev siv dua lossis tso tawm. Ib yam li ntawd, centrifugation siv rau hauv kev lag luam mining rau kev tshem dej tawm, rov qab tau cov zaub mov muaj nqis zoo dua.
Kev Npaj thiab Kev Txij Nkawm ntawm Centrifuges
Yuav kom paub tseeb tias cov txiaj ntsig raug thiab sib xws, kev npaj kom zoo thiab kev saib xyuas tsis tu ncua ntawm cov centrifuges yog qhov tseem ceeb heev. Ua ntej, lub rotor yuav tsum sib npaug, nrog rau cov raj xa dej kuaj teeb tsa sib npaug. Kev tsis sib npaug yuav ua rau muaj kev co, suab nrov, thiab txawm tias ua rau lub centrifuge puas tsuaj. Ntxiv mus, kev kho thiab ntxuav cov rotors, lub hau, thiab lub chamber tsis tu ncua yog qhov tsim nyog los tswj kev ua haujlwm zoo tshaj plaws thiab tiv thaiv kev ua qias tuaj.
Xaus lus:
Kev siv centrifugation ua lub hauv paus tseem ceeb rau kev sib cais cov khoom sib xyaw thoob plaws ntau yam lag luam. Los ntawm kev siv cov ntsiab cai ntawm lub zog centrifugal, cov tshuaj uas muaj qhov sib txawv tuaj yeem raug cais tawm zoo, qhib lawv lub peev xwm tiag tiag. Txij li kev tshawb fawb kho mob mus rau kev siv hauv kev lag luam, qhov ua tau ntau yam ntawm centrifugation ua rau nws yog ib qho cuab yeej tseem ceeb rau kev ua tiav cov khoom zoo thiab ntshiab. Los ntawm kev nkag siab zoo txog kev tshawb fawb tom qab cov txheej txheem no thiab kev saib xyuas tsis tu ncua, cov kws tshawb fawb thiab cov lag luam tuaj yeem txuas ntxiv thawb cov ciam teb ntawm kev tshawb fawb sib cais thiab tsav kev tsim kho tshiab.
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