Ho arola mali ka sentrifugation ho sebelisoa joang ho arola mali

Ho Centrifugation: Mokhoa o Matla oa ho Arola Likarolo tsa Mali

Selelekela:

Mali ke mokelikeli oa bohlokoa o phethang karolo ea bohlokoa ho bolokeng mesebetsi e fapaneng ea 'mele 'meleng oa motho. A na le likarolo tse fapaneng, ho kenyeletsoa lisele tse khubelu tsa mali (RBC), lisele tse tšoeu tsa mali (WBC), li-platelet le plasma. Ho ithuta likarolo tsena ka ho eketsehileng kapa ho lokisa lihlahisoa tse itseng tsa mali bakeng sa merero ea bongaka, ho hlokahala hore li aroloe ka katleho. Mokhoa o mong o sebelisoang haholo bakeng sa morero ona ke ho centrifugation. Sehloohong sena, re tla hlahloba kamoo ho centrifugation ho sebelisoang kateng ho arola likarolo tse fapaneng tsa mali le bohlokoa ba tsona mafapheng a fapaneng.

I. Ho utloisisa Centrifugation:

Ho Centrifugation ke mokhoa oa laboratori o sebelisang matla a centrifugal ho arola lintho tse nang le bongata bo fapaneng ka har'a motsoako. Ho kenyelletsa ho beha motsoako ka har'a centrifuge, mochini o potolohang sampole ka potlako, o etsang hore likarolo tse teteaneng li fallele tlase ho tube ka ho sedimentation. Ts'ebetso ena e lumella ho aroloa le ho bokelloa ha likarolo ka bomong ho latela bongata le boholo ba tsona.

II. Molao-motheo oa Motheo oa ho Centrifugation ka Karohanong ea Mali:

Ho thiba mali ka sekontiri ho sebelisoa haholo ho fumana likarolo tse fapaneng tsa mali, kaha karolo ka 'ngoe e na le matla a fapaneng. Ka ho beha mali tlas'a matla a sekontiri, likarolo tse fapaneng li arohana ka mekhahlelo e fapaneng e ka ntšoang habonolo. Ka tlase ke mehato e amehang karohanong ea mali ka ho thiba mali ka sekontiri:

1. Pokello ea Sampole ea Mali:

Bongata bo loketseng ba mali bo bokelloa ho mokuli ho sebelisoa mekhoa ea aseptic. Mali a fetisetsoa ka har'a lipeipi tse khethehileng tse etselitsoeng ho centrifugation.

2. Tokiso ea Sampole:

Ho thibela ho hoama ha mali, ho eketsoa anticoagulant ka har'a tube e nang le sampole ea mali. Anticoagulant e netefatsa hore mali a lula a le boemong ba metsi nakong ea ts'ebetso ea centrifugation.

3. Ts'ebetso ea ho Centrifugation:

Sampole ea mali e lokisitsoeng e kenngoa ka har'a centrifuge 'me e bilikoa ka lebelo le phahameng. Centrifuge e sebelisa matla a centrifugal, e leng se etsang hore likarolo li arohane ka lebaka la boima ba tsona bo fapaneng. Li-RBC, kaha ke tsona tse boima ka ho fetisisa, li lula tlase, li lateloa ke li-WBC le li-platelet, ha plasma e ntse e le holimo.

III. Karohano ea Lisele tse Khubelu tsa Mali:

Lisele tse khubelu tsa mali (RBC) ke likarolo tse ngata ka ho fetisisa tsa mali, tse ikarabellang bakeng sa ho tsamaisoa ha oksijene 'meleng oohle. Ho arola li-RBC ho mali a felletseng ho bohlokoa mekhoeng e fapaneng ea bongaka, joalo ka tšelo ea mali le ho lokisoa ha lihlahisoa tse itseng tsa mali. Ho centrifugation ho arola li-RBC ka katleho ka ho sebelisa boima ba tsona.

1. Pokello ea Lera le Ruileng la RBC:

Kamora hore ts'ebetso ea ho centrifugation e phethe, li-RBC lia bokellana tlase ho tube, li etsa lera le khubelu le ikhethang. Lera lena le bokelloa ka hloko ho sebelisoa mokhoa oa pipette kapa aspiration.

2. Ho hlatsoa lisele tse khubelu tsa mali:

Li-RBC tse bokelletsoeng li hlatsuoa ka tharollo ea letsoai ho tlosa plasma efe kapa efe e setseng kapa litšila tse sa batleheng e le hore li ka sebelisoa ka mokhoa o sireletsehileng bakeng sa lipatlisiso tse ling kapa merero ea bongaka.

IV. Ho aroloa ha Plasma:

Plasma ke karolo e metsi e mosehla o bobebe maling e nang le liprotheine tse fapaneng, lihormone, li-electrolyte le limolek'hule tse ling tsa bohlokoa. Ho arola plasma maling kaofela ho bohlokoa bakeng sa ho fumana mafu, ho beha leihlo meriana ea phekolo le ho lokisa lihlahisoa tsa mali joalo ka tšelo ea plasma.

1. Pokello ea Lera la Plasma:

Kamora ho centrifugation, lera la plasma le hlaha ka holim'a lisele tse khubelu tsa mali tse pakiloeng. Le bokelloa ka hloko ho sebelisoa pipette kapa mokhoa o tšoanang, ho netefatsa tšilafalo kapa tšitiso e fokolang.

2. Ho thibela ho ruruha ha mali ka lebaka la ho thibela ho ruruha ha mali ka har'a Plasma:

Ho thibela ho hoama ha plasma nakong ea polokelo, ho eketsoa li-anticoagulant tse kang citrate kapa heparin. Lik'hemik'hale tsena li boloka boemo ba mokelikeli 'me li boloka botšepehi ba likarolo tsa plasma.

V. Tšebeliso ea Centrifugation Libakeng tsa Bongaka le Lipatlisiso:

Ho centrifugation ho sebelisoa haholo mafapheng a fapaneng bakeng sa ho arola likarolo tsa mali. Tsena ke lits'ebetso tse 'maloa tse hlahelletseng:

1. Banka ea Mali le Bongaka ba Tšelo ea Mali:

Ho centrifugation ho sebediswa dibankeng tsa madi ho arola le ho lokisa dikarolo tse kang di-RBC tse pakilweng, di-platelet concentrate, le plasma e hoammeng e foreshe. Tsena di bohlokwa bakeng sa tshelo, moo bakudi ba fumanang dihlahiswa tse itseng tsa madi ho latela boemo ba bona ba bongaka.

2. Hematology le K'hemistri ea Kliniki:

Ho centrifugation ke karolo ea bohlokoa ea tlhahlobo ea laboratori, e thusang ho khetholla le ho lekanya likarolo tse fapaneng tsa mali. E nolofalletsa ho lemoha lisele tse sa tloaelehang, tlhahlobo ea serum, le liteko tsa li-biomarker tse itseng.

3. Patlisiso le Nts'etsopele:

Ho sebelisa centrifugation ho sebelisoa haholo lipatlisisong tsa bongaka ho batlisisa likarolo tse fapaneng tsa likarolo tsa mali. Ho nolofalletsa bo-rasaense ho ithuta sebopeho sa sele, ho arola lisele tse itseng tse khahlisang, le ho sekaseka sebopeho sa plasma.

Qetello:

Ho thiba mali ka sekontiri ke mokhoa o matla le o feto-fetohang o sebelisoang haholo ho aroleng likarolo tsa mali. Bokhoni ba oona ba ho sebelisa liphapang tsa bongata bo lumella ho aroloa ha lisele tse khubelu tsa mali, plasma le likarolo tse ling tsa bohlokoa ka katleho. Tšebeliso ea mokhoa ona e ama haholo tlhahlobo ea bongaka, banka ea mali le libaka tse ngata tsa lipatlisiso. Ka tsoelo-pele e tsoelang pele, ho thiba mali ka sekontiri ho tsoela pele ho bapala karolo ea bohlokoa ho ntlafatseng kutloisiso ea rona ea mali le likarolo tsa ona le ho nolofatsa mekhoa ea bongaka e pholosang bophelo.