Yuav ua li cas centrifugation siv los cais cov ntshav
Kev Siv Centrifugation: Ib Txoj Kev Siv Zog Rau Kev Cais Cov Cheebtsam Ntshav
Kev Taw Qhia:
Ntshav yog ib yam kua tseem ceeb uas ua lub luag haujlwm tseem ceeb hauv kev tswj hwm ntau yam kev ua haujlwm ntawm lub cev tib neeg. Nws muaj ntau yam khoom sib txawv, suav nrog cov qe ntshav liab (RBCs), cov qe ntshav dawb (WBCs), cov ntshav platelets, thiab cov ntshav plasma. Yuav kom kawm ntxiv txog cov khoom no lossis npaj cov khoom ntshav tshwj xeeb rau kev kho mob, nws yog ib qho tsim nyog los cais lawv kom zoo. Ib txoj kev siv dav dav rau lub hom phiaj no yog centrifugation. Hauv tsab xov xwm no, peb yuav tshawb nrhiav seb centrifugation siv li cas los cais cov khoom ntshav sib txawv thiab nws qhov tseem ceeb hauv ntau qhov chaw.
I. Kev Nkag Siab Txog Kev Siv Centrifugation:
Kev centrifugation yog ib txoj kev siv hauv chav kuaj uas siv lub zog centrifugal los cais cov khoom sib txawv ntawm qhov sib xyaw. Nws suav nrog kev muab cov khoom sib xyaw rau hauv lub centrifuge, lub tshuab uas tig cov qauv sai sai, ua rau cov khoom sib xyaw ntom dua txav mus rau hauv qab ntawm lub raj los ntawm kev tso dej. Cov txheej txheem no tso cai rau kev cais thiab sau cov khoom sib xyaw raws li lawv qhov ntom thiab qhov loj.
II. Lub Ntsiab Cai Tseem Ceeb ntawm Centrifugation hauv Kev Sib Cais Ntshav:
Kev siv centrifugation dav dav los tau txais ntau yam khoom sib xyaw ntawm cov ntshav, vim tias txhua yam khoom muaj qhov sib txawv. Los ntawm kev siv cov ntshav rau lub zog centrifugal, cov khoom sib txawv sib cais ua ntau txheej sib txawv uas tuaj yeem rho tawm tau yooj yim. Hauv qab no yog cov kauj ruam koom nrog kev sib cais cov ntshav los ntawm kev centrifugation:
1. Kev Sau Cov Qauv Ntshav:
Yuav muab cov ntshav ntim kom txaus los ntawm tus neeg mob siv cov txheej txheem aseptic. Tom qab ntawd cov ntshav yuav raug xa mus rau hauv cov raj tshwj xeeb uas tsim los rau kev centrifugation.
2. Kev Npaj Cov Qauv:
Yuav kom tiv thaiv cov ntshav txhaws, muab tshuaj anticoagulant ntxiv rau hauv lub raj uas muaj cov qauv ntshav. Cov tshuaj anticoagulant ua kom cov ntshav tseem nyob hauv cov kua thaum lub sijhawm centrifugation.
3. Txheej Txheem Centrifugation:
Cov ntshav uas tau npaj lawm raug muab tso rau hauv lub tshuab centrifuge thiab tig nrog qhov ceev ceev. Lub tshuab centrifuge siv lub zog centrifugal, ua rau cov khoom sib cais vim lawv qhov sib txawv ntawm qhov ceev. Cov RBCs, yog qhov hnyav tshaj plaws, nyob hauv qab, ua raws li WBCs thiab platelets, thaum cov plasma tseem nyob rau saum toj.
III. Kev Cais Cov Qe Ntshav Liab:
Cov qe ntshav liab (RBCs) yog cov feem ntau ntawm cov ntshav, uas yog lub luag haujlwm rau kev thauj cov pa oxygen thoob plaws lub cev. Kev cais cov RBCs ntawm cov ntshav tag nrho yog qhov tseem ceeb hauv ntau yam kev kho mob, xws li kev hloov ntshav thiab kev npaj cov khoom ntshav tshwj xeeb. Kev centrifugation ua rau cov RBCs sib cais tau zoo los ntawm kev siv lawv qhov ceev.
1. Kev Sau Cov Txheej RBC-Nplua Nuj:
Tom qab ua tiav cov txheej txheem centrifugation, cov RBCs sib sau ua ke rau hauv qab ntawm lub raj, tsim ib txheej liab sib txawv. Txheej no raug sau ua tib zoo siv lub pipette lossis cov txheej txheem aspiration.
2. Ntxuav cov ntshav liab:
Cov RBCs uas tau sau los raug ntxuav nrog cov kua ntsev kom tshem tawm cov plasma lossis cov khoom tsis huv uas tseem tshuav kom lawv siv tau rau kev tshawb fawb ntxiv lossis kev kho mob.
IV. Kev rho tawm cov ntshav:
Cov ntshav ntshav yog cov ntshav daj daj uas muaj ntau yam protein, cov tshuaj hormones, electrolytes, thiab lwm yam tseem ceeb. Kev cais cov ntshav ntshav tawm ntawm cov ntshav tag nrho yog qhov tseem ceeb rau kev kuaj mob, saib xyuas cov tshuaj kho mob, thiab npaj cov khoom ntshav xws li kev hloov ntshav ntshav.
1. Kev Sau Cov Txheej Plasma:
Tom qab centrifugation, cov txheej plasma tshwm sim saum cov RBCs uas tau ntim. Nws raug sau ua tib zoo siv lub pipette lossis lwm yam txheej txheem zoo sib xws, kom ntseeg tau tias muaj kev ua qias tuaj lossis kev cuam tshuam tsawg kawg nkaus.
2. Tshuaj Anticoagulation ntawm Plasma:
Txhawm rau tiv thaiv kev sib sau ua ke ntawm cov ntshav thaum lub sijhawm khaws cia, cov tshuaj anticoagulants xws li citrate lossis heparin raug ntxiv rau. Cov tshuaj no tswj cov kua thiab khaws cia qhov kev ua haujlwm ntawm cov khoom hauv cov ntshav.
V. Kev Siv Centrifugation Hauv Chaw Kho Mob thiab Kev Tshawb Fawb:
Kev siv centrifugation dav dav hauv ntau yam kev qhuab qhia rau kev cais cov khoom ntshav. Nov yog ob peb daim ntawv thov tseem ceeb:
1. Kev Khaws Ntshav thiab Kev Kho Mob Hloov Ntshav:
Siv centrifugation hauv cov txhab ntshav los cais thiab npaj cov khoom xws li RBCs uas tau ntim, platelet concentrates, thiab cov plasma khov tshiab. Cov no yog qhov tseem ceeb rau kev hloov ntshav, qhov twg cov neeg mob tau txais cov ntshav tshwj xeeb raws li lawv tus mob.
2. Kev Tshawb Fawb Txog Ntshav thiab Kev Tshawb Fawb Txog Tshuaj:
Kev siv lub tshuab centrifugation yog ib feem tseem ceeb ntawm kev kuaj mob hauv chav kuaj mob, pab txheeb xyuas thiab ntsuas cov khoom sib txawv ntawm cov ntshav. Nws ua rau pom cov hlwb tsis zoo, tshuaj xyuas cov ntshav hauv cov ntshav, thiab kuaj cov cim qhia tshwj xeeb.
3. Kev Tshawb Fawb thiab Kev Txhim Kho:
Kev siv centrifugation dav dav hauv kev tshawb fawb txog biomedical los tshawb nrhiav ntau yam ntawm cov khoom xyaw ntawm cov ntshav. Nws ua rau cov kws tshawb fawb muaj peev xwm kawm txog cov qauv ntawm cov hlwb, cais cov hlwb tshwj xeeb uas xav tau, thiab tshuaj xyuas cov khoom sib xyaw ntawm cov ntshav.
Xaus lus:
Kev siv centrifugation yog ib txoj kev siv zog thiab siv tau ntau yam los cais cov ntshav. Nws lub peev xwm los siv qhov sib txawv ntawm qhov ceev ua rau muaj kev cais cov qe ntshav liab, plasma, thiab lwm yam tseem ceeb. Kev siv txoj kev no muaj feem cuam tshuam loj heev rau kev kuaj mob, kev khaws ntshav, thiab ntau qhov chaw tshawb fawb. Nrog rau kev nce qib tas mus li, kev siv centrifugation tseem ua lub luag haujlwm tseem ceeb hauv kev txhim kho peb txoj kev nkag siab txog ntshav thiab nws cov khoom thiab hauv kev pab txhawb rau cov txheej txheem kho mob cawm txoj sia.
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