kedu akụkụ ahụ dị iche iche nke kewara na centrifugation ọ bụla

Ịmepụta ihe: Ịkpughe Ebe E Zoro Ezo nke Organelles

Okwu Mmalite:

Centrifugation bụ usoro dị ike e ji eme ihe n'ọtụtụ ngalaba sayensị dị iche iche, na-enye ohere ka e kewaa akụkụ dị iche iche nke mkpụrụ ndụ. N'ụwa na-adọrọ mmasị nke bayoloji mkpụrụ ndụ, organelles na-arụ ọrụ dị mkpa ma ịghọta ọrụ ha dị oke mkpa. Isiokwu a na-enyocha usoro na-adọrọ mmasị nke centrifugation ma na-ekpughe organelles nke enwere ike ikewapụ nke ọma site na iji usoro a.

1. Ịghọta Centrifugation:

Ntinye ihe n'ime centrifuge bụ usoro nke na-eji ike nke centrifugal acceleration kewaa ihe dị n'ime ngwakọta dabere na njupụta ha. Site n'itinye ngwakọta ahụ n'ime ntụgharị ọsọ dị elu n'ime centrifuge, ihe ndị dị arọ na-agagharị na ala, na-emepụta pellet, ebe ihe ndị dị mfe na-anọgide na supernatant. Nkewa a na-enye ndị ọkà mmụta sayensị ohere ikewapụ ma mụọ ihe dị n'ime ya nke ọ bụla.

2. Ike nke Ntinye Ọdịiche:

Usoro dị iche iche nke centrifugation bụ usoro a na-ejikarị ekewa organelles dabere na ọnụego sedimentation ha. Site n'iji usoro ntụgharị dị ala na ọsọ dị elu, usoro a na-enye ohere maka ikewapụ organelles dị iche iche n'usoro, dabere na njupụta ha. N'ụzọ dị otu a, enwere ike kewaa ngwakọta dị mgbagwoju anya n'ime ihe dị iche iche, nke ọ bụla nwere organelles pụrụ iche.

2.1 Nuclei na Mitochondria na Mbibi Mbụ:

A na-eji centrifugation mbụ na obere ọsọ kewaa organelles buru ibu dịka nuclei na mitochondria. organelles ndị a nwere njupụta dị elu ma nwee ike ịla n'iyi na ọsọ dị nwayọ. Mgbe ntụgharị mbụ gasịrị, ihe na-asọfe n'elu ya nwere akụkụ cytoplasmic, ebe pellet nwere nuclei na mitochondria.

2.2 Endoplasmic Reticulum na Golgi Apparatus na Nke Abụọ Spin:

Ihe na-esi n'elu mmiri nke e nwetara mgbe agbagharị mbụ gasịrị, nke nwere akụkụ cytoplasmic, nwere ike ịgafe ọsọ ọsọ iji mee ka pelletize endoplasmic reticulum (ER) na ngwa Golgi. Akụkụ ndị a, bụ ndị na-ahụ maka mmepụta protein, nhazi, na ibugharị, nwere njupụta dị n'etiti. Ya mere, a na-ekewa ha na usoro centrifugation nke abụọ a.

3. Ịkewapụ Lysosomes na Peroxisomes:

Lysosomes na peroxisomes, ndị dị mkpa maka mbibi nke macromolecules na iwepụ ihe ndị na-emerụ ahụ, n'otu n'otu, bụ akụkụ abụọ dị mkpa nke chọrọ usoro pụrụ iche maka nkewa.

3.1 Njupụta Gradient Centrifugation maka Lysosomes:

Iji kewapụ lysosomes, a na-ejikarị centrifugation gradient njupụta eme ihe. Usoro a gụnyere ịmepụta gradient nke njupụta na-abawanye, yana ngwọta kachasị njupụta n'ala na nke kachasị mfe n'elu. Mgbe a na-etinye ngwakọta nwere lysosomes centrifuge, organelles ndị a na-aga na mpaghara njupụta ha kpọmkwem n'ime gradient, na-ekwe ka ha kewapụ na ihe ndị ọzọ.

3.2 Ultracentrifugation maka Peroxisomes:

N'ihi myirịta dị n'etiti peroxisomes na organelles ndị ọzọ, a chọrọ ụzọ ọzọ maka ikewapụ ha. Enwere ike iji Ultracentrifugation, site na iji ọsọ dị elu, kewapụ peroxisomes na ngwakọta ndị ọzọ. Site n'iji obere ọdịiche dị na njupụta, enwere ike ịkpụ peroxisomes n'ụzọ dị iche iche, na-eme ka ọmụmụ na nyocha ha dịkwuo mfe.

4. Echiche na Ọganihu n'Ọdịnihu:

Ka teknụzụ na-aga n'ihu, ọganihu ọhụrụ na usoro centrifugation na-eme ka nghọta anyị banyere organelles na ọrụ ha dịkwuo mma. Mmepe ọhụrụ ndị a na-eme site na iji isopycnic density gradient centrifugation na iji nanotechnology enyerela aka nke ukwuu na nhazi na arụmọrụ nke nkewa organelle, na-eme ka ha bụrụ ngwaọrụ dị ike maka nyocha bayoloji sel.

Mmechi:

Centrifugation na-arụ ọrụ dị mkpa n'ịghọta usoro ihe owuwu sel dị mgbagwoju anya site n'ikwe ka organelles kewaa. Site na nuclei ruo mitochondria, endoplasmic reticulum ruo golgi apparatus, lysosomes ruo peroxisomes, organelles ndị a nke ọ bụla nwere ọrụ na usoro pụrụ iche. Usoro centrifugation, yana ụzọ ọhụrụ, na-enyere ndị ọkà mmụta sayensị aka inyocha ụwa dị mgbagwoju anya nke sel, na-emepe ihe nzuzo zoro ezo n'ime organelles ha.