he aha nā hui e hiki ke hoʻokaʻawale ʻia e ka centrifugation
Hoʻolauna i ka Centrifugation a me kona koʻikoʻi i ka hoʻokaʻawale ʻana i nā hui ʻana
He ʻenehana hoʻokolohua i hoʻohana nui ʻia ka centrifugation no ka hoʻokaʻawale ʻana i nā hui ʻana ma muli o ko lākou mau mānoanoa. Hoʻohana ia i nā kumumanaʻo o ka ikaika centrifugal e hoʻokaʻawale koke a maikaʻi i nā ʻāpana i loko o kahi hui ʻana. ʻO ka neʻe wili wikiwiki o kahi centrifuge e hana i kahi ikaika ʻupena i kuhikuhi ʻia mai ke kikowaena o ka wili ʻana, e hiki ai i nā ʻāpana a i ʻole nā mea o nā mānoanoa like ʻole ke hoʻokaʻawale ma muli o ka nui o ka sedimentation.
ʻO kekahi o nā pono nui o ka centrifugation ʻo ia kona hiki ke hoʻokaʻawale i nā hui ʻana i paʻakikī a hiki ʻole paha ke hoʻokaʻawale me ka hoʻohana ʻana i nā ʻano kuʻuna e like me ka kānana a i ʻole ka decantation. Ma kēia ʻatikala, e ʻimi mākou i nā hui like ʻole e hiki ke hoʻokaʻawale pono ʻia e ka centrifugation, e hōʻike ana i ke koʻikoʻi o kēia ʻano hana ma nā noiʻi ʻepekema a me nā noi ʻoihana.
Ka hoʻokaʻawale ʻana i nā ʻāpana koko no nā diagnostics lapaʻau
He kuleana koʻikoʻi ko ka centrifugation i nā diagnostics lapaʻau, ʻoi aku hoʻi ma ke kahua o ka hematology. Hiki ke hoʻokaʻawale ʻia ke koko, kahi hui paʻakikī o nā cell, platelets, a me ka plasma, i loko o kona mau ʻāpana like ʻole no ka nānā ʻana. Ma ka hoʻohana ʻana i ka ikaika centrifugal, hiki ke hoʻokaʻawale pono ʻia nā cell koko ʻulaʻula (RBC), nā cell koko keʻokeʻo (WBC), a me nā platelets mai ka plasma.
ʻO ke ʻano hana centrifugation ʻokoʻa, i ʻike ʻia ʻo ka density gradient centrifugation, kōkua i ka hoʻokaʻawale ʻana i nā WBC mai nā RBC. Hoʻopaʻa ʻia ka hui ʻana ma luna o kahi medium gradient me ka density kiʻekiʻe ma mua o nā cell koko ʻulaʻula akā haʻahaʻa ma mua o nā cell koko keʻokeʻo. Ma hope o ka centrifugation, e neʻe nā WBC i ka interface kahi e kūlike ai ka density o ka medium gradient me kā lākou iho, e hiki ai iā lākou ke hoʻokaʻawale mai nā RBC.
Ka Hoʻokaʻawale ʻana o nā ʻĀpana Kelepona a me nā Organelles no nā Kumu Noiʻi
Hoʻohana pinepine nā kānaka noiʻi i ka centrifugation e unuhi i nā ʻāpana cellular a i ʻole nā organelles no ka nānā hou aku. No ka laʻana, hiki ke hoʻokaʻawale ʻia ka mitochondria, nā hale mana o ke kelepona, me ka hoʻohana ʻana i ka centrifugation ʻokoʻa. Ma ka hoʻololi ʻana i ka wikiwiki a me ka manawa centrifugation, hiki i nā kānaka noiʻi ke hoʻokaʻawale i nā ʻāpana mitochondrial mai ke koena o nā ʻāpana cellular.
Pēlā nō, he mea koʻikoʻi ka centrifugation i ka hoʻokaʻawale ʻana i nā protein cellular, nā nucleic acid, a me nā macromolecules ʻē aʻe mai nā lysates cell crude. ʻO ka Ultracentrifugation, me ka hoʻohana ʻana i nā wikiwiki kiʻekiʻe a me nā manawa centrifugation lōʻihi, hiki ke hoʻokaʻawale i nā ʻāpana subcellular ma muli o ko lākou nui, ke ʻano, a me ka density. Loaʻa i kēia ʻenehana nā noi i ke aʻo ʻana i nā kaʻina hana cellular, ka hoʻoholo ʻana i nā ʻano biomolecular, a me ka hoʻomohala ʻana i nā hana therapeutic.
Ka hoʻokaʻawale ʻana o nā mea paʻa i hoʻokuʻu ʻia mai nā wai i ka ʻoihana meaʻai a me ka mea inu
Hoʻohana ʻia ka centrifugation ma nā ʻoihana like ʻole e hoʻokaʻawale i nā mea paʻa i hoʻokuʻu ʻia mai nā wai. I ka ʻoihana meaʻai a me ka mea inu, he kuleana koʻikoʻi kona i ka hoʻomālamalama ʻana a me ka hoʻomaʻemaʻe ʻana i nā wai, nā waina, a me nā wai ʻē aʻe e hoʻopau ʻia. Ma ke kau ʻana i ka hui ʻana i nā ikaika centrifugal kiʻekiʻe, e noho ana nā ʻāpana kaumaha ma lalo, e waiho ana i kahi hapa wai maopopo.
Eia kekahi, hoʻohana nui ʻia nā mea hoʻokaʻawale centrifugal i ka ʻoihana waiū e hoʻokaʻawale i ka kirīmi mai ka waiū, e hiki ai ke hana ʻia ka waiū momona haʻahaʻa. Ma o ka centrifugation, hiki ke hoʻokaʻawale pono ʻia ka kirīmi, ʻoiai ʻaʻole i mānoanoa ma mua o ka waiū. He mea nui kēia kaʻina hana i ka hana ʻana i nā huahana waiū like ʻole e like me ka pata, ka tīhi, a me ka yogurt.
Ka Hoʻokaʻawale ʻana i nā ʻĀpana mai nā Laʻana Kaiapuni no ka Nānā ʻana
He mea nui ka centrifugation i nā ʻepekema kaiapuni no ka nānā ʻana i ka lepo, ka sediment, a me nā laʻana wai. Hiki iā ia ke ʻae i nā mea noiʻi e hoʻopili i nā ʻāpana a i ʻole nā microorganism i manaʻo ʻia mai nā matrices kaiapuni paʻakikī a hoʻoheheʻe ʻia, e kōkua ana i kā lākou ʻike ʻana a me ka nānā ʻana.
No ka laʻana, i loko o nā hale hana hoʻomaʻemaʻe wai, hoʻohana ʻia nā ʻano hana centrifugation e wehe i nā ʻāpana i hoʻokuʻu ʻia, e like me ka algae, bacteria, a me ka sediment, mai ka wai kahe ma mua o nā hana hoʻomaʻemaʻe hou aʻe. Kōkua kēia i ka hōʻemi ʻana i ka turbidity o ka wai a me ka hōʻoia ʻana i ka wehe ʻana i nā pathogens hiki ke loaʻa.
Hopena:
He ʻano hana maʻalahi ka centrifugation no ka hoʻokaʻawale ʻana i nā hui ʻana ma muli o nā ʻano kino like ʻole. Loaʻa kāna mau noi ma nā ʻano like ʻole, me nā diagnostics lapaʻau, ka noiʻi ʻepekema, a me nā kaʻina hana ʻoihana. Ma ka hoʻohana ʻana i ka mana o ka ikaika centrifugal, hiki ke hoʻokaʻawale pono ʻia nā hui like ʻole, e hoʻomākaukau ana i ke ala no ka nānā hou ʻana, ka hoʻomaʻemaʻe ʻana, a me ka hoʻohana ʻana i nā ʻāpana like ʻole.
.