ke metsoako efe e ka aroloang ka centrifugation

Selelekela sa Centrifugation le Bohlokoa ba Yona ho Aroleng Metswako

Ho Centrifugation ke mokhoa o sebelisoang haholo oa laboratori bakeng sa ho arola metsoako ho latela bongata ba eona. E sebelisa melao-motheo ea matla a centrifugal ho arola likarolo ka potlako le ka katleho ka har'a motsoako. Motsamao o potolohang ka lebelo le phahameng oa centrifuge o hlahisa matla a letlooa a lebisitsoeng hole le bohareng ba potoloho, e leng se nolofalletsang likaroloana kapa lintho tse nang le bongata bo fapaneng ho arohana ho latela sekhahla sa ho senngoa ha mobu.

E 'ngoe ea melemo e meholo ea ho centrifugation ke bokhoni ba eona ba ho arola metsoako eo ho seng joalo e neng e tla ba thata kapa e ke keng ea khoneha ho e arola ho sebelisoa mekhoa ea setso joalo ka ho sefa kapa ho tlosa litšila. Sehloohong sena, re tla hlahloba metsoako e fapaneng e ka aroloang ka katleho ka ho centrifugation, re totobatsa bohlokoa ba mokhoa ona lipatlisisong tsa mahlale le lits'ebetsong tsa indasteri.

Karohano ea Likarolo tsa Mali bakeng sa Tlhahlobo ea Bongaka

Ho centrifugation ho phetha karolo ea bohlokoa tlhahlobong ea bongaka, haholo-holo lefapheng la hematology. Mali, motsoako o rarahaneng oa lisele, li-platelet le plasma, a ka aroloa ka likarolo tsa 'ona tse fapaneng bakeng sa tlhahlobo. Ka ho sebelisa matla a centrifugal, lisele tse khubelu tsa mali (RBC), lisele tse tšoeu tsa mali (WBC), le li-platelet li ka aroloa ka katleho ho plasma.

Mokhoa oa ho arola lisele tse tšoeu tsa mali ka mokhoa o fapaneng, o tsejoang e le density gradient centrifugation, o thusa ho arola lisele tse tšoeu tsa mali ho lisele tse khubelu tsa mali. Motsoako ona o kenngoa holim'a gradient medium e nang le density e phahameng ho feta ea lisele tse khubelu tsa mali empa e le tlase ho feta ea lisele tse tšoeu tsa mali. Ha e kenngoa ka centrifugation, lisele tse tšoeu tsa mali li tla fallela sebakeng seo ho sona density ea lisele tse khubelu tsa mali e lumellanang le ea tsona, e leng se nolofalletsang karohano ea tsona le lisele tse khubelu tsa mali.

Karohano ea Likarolo tsa Sele le Li-organelle bakeng sa Merero ea Lipatlisiso

Hangata bafuputsi ba itšetleha ka ho centrifugation ho ntša likarolo tse itseng tsa sele kapa li-organelle bakeng sa tlhahlobo e eketsehileng. Mohlala, mitochondria, e leng matlo a matla a sele, e ka aroloa ka ho sebelisa ho centrifugation e fapaneng. Ka ho fetola lebelo le nako ea ho centrifugation ka mokhoa o hlophisehileng, bafuputsi ba ka arola likaroloana tsa mitochondrial ho tsoa likarolong tse ling tsa sele.

Ka ho tšoanang, ho centrifugation ho bohlokoa ho aroleng liprotheine tsa sele, li-nucleic acid, le li-macromolecule tse ling ho tsoa ho li-lysate tsa sele tse tala. Ho centrifugation ka mokhoa oa Ultracentrifugation, ho sebelisa lebelo le phahameng le linako tse telele tsa ho centrifugation, ho nolofalletsa karohano ea likaroloana tse ka tlas'a sele ho latela boholo ba tsona, sebopeho le bongata. Mokhoa ona o fumana lits'ebetso ho ithuteng lits'ebetso tsa sele, ho fumana meaho ea limolek'hule, le ho nts'etsapele mekhoa ea phekolo.

Karohano ea Lintho tse Chesitsoeng tse Leketlileng ho Metsi a Indastering ea Lijo le Lino

Ho centrifugation ho sebediswa diindastering tse fapaneng ho arola dintho tse tiileng tse emisitsweng ho tse metsi. Indastering ya dijo le dino, e bapala karolo ya bohlokwa ho hlakiseng le ho hlwekiseng lero, veine le tse ding tse ka jewang. Ka ho beha motswako tlasa matla a maholo a centrifugal, dikarolwana tse boima di dula tlase, di siya karolo e hlakileng ya mokelikeli.

Ho phaella moo, di-centrifugal divider di sebediswa haholo indastering ya lebese ho arola tranelate ho lebese, e leng se nolofalletsang tlhahiso ya lebese le nang le mafura a tlase. Ka ho fokotsa mafura, tranelate, kaha ha e teteaneng hakaalo ho feta lebese, e ka fokotswa ka katleho. Tshebetso ena e bohlokwa ho hlahiseng mefuta e fapaneng ya dihlahiswa tsa lebese tse kang botoro, chisi le yogurt.

Karohano ea Likaroloana ho Mehlala ea Tikoloho bakeng sa Tlhahlobo

Ho centrifugation ho bohlokoa haholo mahlaleng a tikoloho bakeng sa ho sekaseka disampole tsa mobu, sediment le metsi. E dumella bafuputsi ho tsepamisa maikutlo dikarolwana kapa dikokwana-hloko tse rerilweng ho tswa ho matrices e rarahaneng le e hlapolotsweng ya tikoloho, ho thusa ho di lemoha le ho di sekaseka.

Mohlala, lifemeng tsa kalafo ea metsi, mekhoa ea ho centrifugation e sebelisoa ho tlosa likaroloana tse leketlileng, tse kang bolele, libaktheria le sediment, metsing a nang le tšusumetso pele ho mehato e meng ea ho hloekisa. Sena se thusa ho fokotsa ho fifala ha metsi le ho netefatsa hore ho tlosoa likokoana-hloko tse ka bang teng.

Qetello:

Ho centrifugation ke mokhoa o feto-fetohang oa ho arola metsoako ho latela thepa e fapaneng ea 'mele. Ts'ebeliso ea eona e akaretsa masimo a fapaneng, ho kenyeletsoa le tlhahlobo ea bongaka, lipatlisiso tsa mahlale le lits'ebetso tsa indasteri. Ka ho sebelisa matla a matla a centrifugal, metsoako e fapaneng e ka aroloa ka katleho, e bula tsela bakeng sa tlhahlobo e eketsehileng, tlhoekiso le ts'ebeliso ea likarolo tse fapaneng.