zeziphi iingxube ezinokwahlulwa nge-centrifugation
Intshayelelo kwiCentrifugation kunye nokubaluleka kwayo ekwahlukaniseni imixube
I-Centrifugation yindlela esetyenziswa kakhulu kwelebhu yokwahlula imixube ngokusekelwe kubuninzi bayo. Isebenzisa imigaqo yamandla e-centrifugal ukwahlula ngokukhawuleza nangokufanelekileyo izinto ngaphakathi komxube. Intshukumo yokujikeleza ngesantya esiphezulu ye-centrifuge idala amandla angenanto ajoliswe kude nombindi wokujikeleza, nto leyo evumela amasuntswana okanye izinto ezinobunzima obahlukeneyo ukuba zahlukane ngokusekelwe kwisantya sokutyibilika.
Enye yezona nzuzo ziphambili ze-centrifugation kukukwazi kwayo ukwahlula imixube ebekuya kuba nzima okanye akunakwenzeka ukuyihlukanisa kusetyenziswa iindlela zemveli ezifana nokuhluza okanye ukususa ulwelo. Kweli nqaku, siza kuhlola imixube eyahlukeneyo enokwahlulwa ngempumelelo nge-centrifugation, sigqamise ukubaluleka kwale ndlela kuphando lwesayensi nakwimisebenzi yemizi-mveliso.
Ukwahlulwa kweMicandelo yeGazi ukuze kufunyanwe uxilongo lwezonyango
I-Centrifugation idlala indima ebalulekileyo ekuxilongweni kwezonyango, ingakumbi kwicandelo le-hematology. Igazi, umxube ontsonkothileyo weeseli, iiplatelets, kunye ne-plasma, linokwahlulwa libe ziinxalenye zalo ezahlukeneyo ukuze kuhlalutywe. Ngokusebenzisa amandla e-centrifugal, iiseli ezibomvu zegazi (ii-RBC), iiseli ezimhlophe zegazi (ii-WBC), kunye neeplatelets zinokwahlulwa ngokufanelekileyo kwi-plasma.
Indlela yokwahlulahlula i-centrifugation, eyaziwa ngokuba yi-density gradient centrifugation, inceda ekwahlukaniseni ii-WBC kwii-RBC. Lo mxube ubekwa kwi-gradient medium enoxinano oluphezulu kunolweeseli ezibomvu zegazi kodwa oluphantsi kunolweeseli ezimhlophe zegazi. Xa i-centrifugation isasazeka, ii-WBC ziya kufudukela kwi-interface apho uxinano lwe-gradient medium luhambelana nolwazo, nto leyo evumela ukwahlukana kwazo nee-RBC.
Ukwahlulwa kwezixhobo zeSeli kunye neeOrganelles ngeenjongo zoPhando
Abaphandi badla ngokuxhomekeka kwi-centrifugation ukuze bakhuphe izinto ezithile zeseli okanye ii-organelles ukuze bahlalutye ngakumbi. Umzekelo, i-mitochondria, iindawo zamandla zeseli, inokwahlulwa kusetyenziswa i-centrifugation eyahlukileyo. Ngokutshintsha isantya kunye nexesha le-centrifugation ngokucwangcisiweyo, abaphandi banokwahlula iinxalenye ze-mitochondrial kwezinye izinto zeseli.
Ngokufanayo, i-centrifugation ibalulekile ekuhlukaniseni iiproteni zeseli, ii-nucleic acids, kunye nezinye ii-macromolecules kwi-crude cell lysates. I-Ultracentrifugation, esebenzisa isantya esiphezulu kunye namaxesha amade e-centrifugation, ivumela ukwahlula amasuntswana angaphantsi kweseli ngokusekelwe kubukhulu bawo, imo, kunye noxinano. Le ndlela ifumana ukusetyenziswa ekufundeni iinkqubo zeseli, ekumiseleni izakhiwo ze-biomolecular, nasekuphuhliseni iindlela zonyango.
Ukwahlulwa kwezinto eziqinileyo ezixutyiweyo kwizinto ezingamanzi kwishishini lokutya neziselo
I-Centrifugation isetyenziswa kumashishini ahlukeneyo ukwahlula izinto eziqinileyo ezixotyulweyo kwizinto ezimanzi. Kwishishini lokutya neziselo, idlala indima ebalulekileyo ekucaciseni nasekucoceni iijusi, iiwayini, kunye nezinye izinto ezisetyenziswayo. Ngokubeka umxube kumandla aphezulu e-centrifugal, amasuntswana anzima ahlala ezantsi, eshiya ngasemva iqhekeza elicacileyo lolwelo.
Ukongeza, izixhobo zokwahlulahlula i-centrifugal zisetyenziswa kakhulu kwishishini lobisi ukwahlula i-cream kubisi, nto leyo evumela ukuveliswa kobisi olunamafutha aphantsi. Ngokusebenzisa i-centrifugation, i-cream, ekubeni incinci kakhulu kunobisi, inokususwa ngempumelelo. Le nkqubo ibalulekile ekuveliseni uluhlu lweemveliso zobisi ezifana nebhotolo, itshizi kunye neyogathi.
Ukwahlulwa kweeNgcambu kwiisampuli zeNdalo ukuze kuhlalutywe
Ukucoca i-centrifugation luncedo kakhulu kwisayensi yokusingqongileyo ekuhlalutyeni iisampulu zomhlaba, intlenga, kunye namanzi. Ivumela abaphandi ukuba bagxile kwiisuntswana okanye iintsholongwane ekujoliswe kuzo ezivela kwi-matrices ezintsonkothileyo nezixutyiweyo, nto leyo enceda ekufumaneni nasekuhlalutyeni kwazo.
Umzekelo, kwizityalo zokunyanga amanzi, iindlela zokucoca amanzi zisetyenziselwa ukususa amasuntswana axhonyiweyo, anje nge-algae, iibhaktheriya, kunye ne-sediment, emanzini achaphazelekileyo ngaphambi kokuba kuthathwe amanyathelo okucoca amanzi. Oku kunceda ekunciphiseni ukungcoliswa kwamanzi nokuqinisekisa ukususwa kwezifo ezinokubakho.
Isiphelo:
I-Centrifugation yindlela eguquguqukayo yokwahlula imixube ngokusekelwe kwiimpawu ezahlukeneyo zomzimba. Ukusetyenziswa kwayo kugubungela iinkalo ezahlukeneyo, kubandakanya ukuxilongwa kwezonyango, uphando lwesayensi, kunye neenkqubo zoshishino. Ngokusebenzisa amandla e-centrifugal force, imixube eyahlukeneyo inokwahlulwa ngokufanelekileyo, ivule indlela yohlalutyo olongezelelweyo, ukucoca, kunye nokusetyenziswa kwezinto ezahlukeneyo.
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