Hiki i kahi centrifuge ke hoʻokaʻawale i nā protein mai nā lipids

Hoʻolauna: Ke hoʻomaopopo nei i ke kuleana o ka Centrifugation i ka hoʻokaʻawale ʻana o ka Protein-Lipid

He ʻano hana koʻikoʻi ka centrifugation i hoʻohana ʻia ma nā ʻano ʻepekema like ʻole, me ka biochemistry a me ka biology molecular, no ka hoʻokaʻawale ʻana i nā mea ma muli o ko lākou nui. I ka wā e pili ana i ka hoʻokaʻawale ʻana i nā protein mai nā lipids, hiki i ka centrifugation ke lilo i mea hana ikaika. Ke ʻeli nei kēia ʻatikala i nā mea paʻakikī o ka hoʻokaʻawale ʻana o ka protein-lipid me ka hoʻohana ʻana i kahi centrifuge a hōʻike i kāna mau noi a me nā pilikia. Me ka hiki ke hoʻololi i ka noiʻi ma nā ʻano like ʻole, lilo ka hoʻomaopopo ʻana i nā hiki a me nā palena o kēia ʻano hana i mea nui.

I. Nā Kumu o ka Centrifugation: Ke wehe nei i nā Mana ma hope o ka hoʻokaʻawale ʻana

Hoʻohana ka centrifugation i ka ikaika centrifugal e hoʻokaʻawale i nā ʻāpana like ʻole i loko o kahi laʻana ma muli o ko lākou nuipa a i ʻole ka nui. ʻŌlelo ke kumumanaʻo o ka ikaika centrifugal e emi koke nā ʻāpana ʻoi aku ka nui ma mua o nā ʻāpana māmā. Eia nō naʻe, hiki i ka hoʻokaʻawale ʻana i nā protein mai nā lipids ke lilo i hana paʻakikī ma muli o ko lākou mau nui like. No ka hoʻokō ʻana i ka hoʻokaʻawale holomua, pono e noʻonoʻo ʻia nā mea ʻē aʻe e like me ka nui, ke ʻano, a me ka solubility.

II. Centrifugation ʻokoʻa: Hoʻohana ʻana i nā ʻokoʻa o ka nui a me ka nui

ʻO ke centrifugation ʻokoʻa kahi ʻano hana i hoʻohana nui ʻia e hoʻokaʻawale i nā protein mai nā lipids. Hoʻohana ia i nā ʻokoʻa o ka nui a me ka nui ma waena o nā ʻāpana. I ka wā mua, hana ʻia kahi hana centrifugation haʻahaʻa e hoʻokaʻawale i nā cell holoʻokoʻa a i ʻole nā ​​​​​​hoʻohuihui ʻiʻo mai nā ʻōpala nui a me nā nuclei. Ma hope o kēia, ua ukali ʻia e nā centrifugations wikiwiki kiʻekiʻe, e ʻae ai i ka hoʻokaʻawale ʻana o nā organelles a me nā ʻāpana subcellular, e like me mitochondria a i ʻole endoplasmic reticulum.

III. Ke Keʻena Centrifugation Gradient Density: He Laina Maikaʻi ma waena o ka Pono a me ka Paʻakikī

ʻO ka centrifugation gradient density kekahi ʻano hana i hoʻohana ʻia no ka hoʻokaʻawale ʻana o ka protein-lipid. Ma kēia ʻano hana, ua kau ʻia nā laʻana ma luna o kahi medium gradient density, e like me ka sucrose a i ʻole cesium chloride, i loko o kahi paipu centrifuge. I ka wā e centrifugation ai ka laʻana, hele nā ​​​​mea ma o ka gradient, e hoʻokaʻawale ana ma muli o ko lākou density buoyant. Hiki i kēia ʻano hana ke hoʻokaʻawale i nā ʻano palupalu, e like me nā lipoproteins, nona nā protein a me nā lipids.

IV. Nā Pilikia i ka Hoʻokaʻawale ʻana o ka Protein-Lipid: Ke Lanakila nei i ke Kūlana Paʻa

ʻO kekahi o nā pilikia kūlohelohe i ka hoʻokaʻawale ʻana i nā protein mai nā lipids aia i ko lākou hui pū ʻana. Hoʻopili pinepine nā protein a me nā lipids a hana i nā paʻakikī, e paʻakikī ai ke hoʻokaʻawale iā lākou ma o ka centrifugation wale nō. No ka hoʻoponopono ʻana i kēia pilikia, hiki ke hoʻohana ʻia nā detergents mālie a i ʻole nā ​​​​​​lāʻau hoʻoheheʻe organik e hoʻopilikia i nā pilina protein-lipid. Eia nō naʻe, ʻo ka loaʻa ʻana o nā kūlana kūpono e mālama ai i ka pono o ka laʻana me ka hoʻomaʻamaʻa ʻana i ka hoʻokaʻawale ʻana he hana hoʻāʻo a hewa.

V. Nā ʻenehana Centrifugation holomua: Ma ʻō aku o nā ʻano hana maʻamau

Ke hoʻomohala mau nei nā kānaka noiʻi i nā ʻano hana centrifugation holomua e hoʻonui i ka pono o ka hoʻokaʻawale ʻana o ka protein-lipid. ʻO kekahi o ia ʻano hana, ʻo ia ka centrifugation zonal a i ʻole isopycnic, kahi e hoʻohana ai i nā gradients density me nā papa he nui e hoʻokaʻawale i nā molekala o nā densities like ʻole. ʻAe kēia ʻano hana i ka fractionation pololei akā pono nā lako hana kūikawā a me ka ʻike loea e hana pono ai. Eia kekahi, ʻo nā ʻano hana hou e like me ka ultracentrifugation a me ka centrifugation super-speed e hāʻawi paha i nā ala hou e hoʻoponopono ai i nā hiʻohiʻona hoʻokaʻawale paʻakikī.

VI. Nā Hoʻohana: Ke Wehe ʻana i nā ʻAno Hana Kelepona a me nā Alanui Maʻi

He hopena koʻikoʻi ka hoʻokaʻawale ʻana o ka protein-lipid me ka hoʻohana ʻana i ka centrifugation ma nā wahi noiʻi like ʻole. No ka laʻana, ʻo ka hoʻomaopopo ʻana i ke ʻano o ka launa pū ʻana o nā protein me nā lipid i loko o nā membrane cellular e hōʻike ana i nā ʻike koʻikoʻi i ka transduction hōʻailona cellular a me nā ala maʻi. Ma ka hoʻokaʻawale ʻana i nā protein a i ʻole lipids kikoʻī, hiki i nā kānaka ʻepekema ke wehe i kā lākou kuleana i nā kaʻina hana physiological e like me ka pelu ʻana o ka protein, ka neʻe ʻana o nā cell, a me ka mahele ʻana o nā cell.

Hopena: He ʻenehana ikaika i ka noiʻi Protein-Lipid

He mea hana maʻalahi ka centrifugation no ka hoʻokaʻawale ʻana i nā protein mai nā lipids, ʻoiai he mau pilikia ia ma muli o ko lākou mau densities like a me nā pilina. Ma ka hoʻohana ʻana i nā ʻano hana centrifugation like ʻole e like me ka differential centrifugation a me ka density gradient centrifugation, hiki i nā mea noiʻi ke hoʻokaʻawale a hoʻokaʻawale pono i nā protein a me nā lipids mai nā laʻana paʻakikī. ʻO ka holomua mau ʻana i ka ʻenehana centrifugation e hoʻomākaukau i ke ala no nā ʻano hana hoʻokaʻawale protein-lipid ʻoi aku ka pololei a me ka ikaika, e hiki ai ke ʻike i nā ʻano hana cellular a me nā ala maʻi.