na sekontirifu se ka arola liprotheine ho mafura

Selelekela: Ho Utloisisa Karolo ea Centrifugation ho Karohano ea Protheine le Lipid

Ho centrifugation ke mokhoa oa motheo o sebelisoang mafapheng a fapaneng a saense, ho kenyeletsoa biochemistry le baeloji ea limolek'hule, bakeng sa ho arola lintho ho latela bongata ba tsona. Ha ho tluoa tabeng ea ho arola liprotheine ho tsoa ho lipids, ho centrifugation e ka ba sesebelisoa se matla. Sengoloa sena se hlahloba ka botebo mathata a karohano ea protheine le lipid ho sebelisoa centrifuge 'me se totobatsa lits'ebetso le liphephetso tsa eona. Ka bokhoni ba ho fetola lipatlisiso libakeng tse 'maloa, ho utloisisa bokhoni le mefokolo ea mokhoa ona ho ba bohlokoa haholo.

I. Metheo ea ho Centrifugation: Ho Senola Matla a Ka mor'a Karohano

Ho centrifugation ho sebedisa matla a centrifugal ho arola dikarolo tse fapaneng ka hara sampole ho latela boima ba tsona kapa bongata ba tsona. Molao-motheo wa matla a centrifugal o bolela hore dikarolwana tse teteaneng di tla tsholoha ho ya tlase ho tube ka lebelo le potlakileng ho feta dikarolwana tse bobebe. Leha ho le jwalo, ho arola diprotheine ho lipids e ka ba mosebetsi o rarahaneng ka lebaka la bongata ba tsona bo tshwanang. Ho fihlella karohano e atlehileng, dintlha tse ding tse kang boholo, sebopeho le ho qhibiliha di hloka ho nahanelwa.

II. Phapang ea ho Fokotsa Matla: Ho Sebelisa Botenya le Phapang ea Boholo

Ho arola dipharologanyo tsa dipharologanyo ke mokgwa o sebediswang haholo ho arola diprotheine ho lipids. E nka monyetla ka dipharologanyo tsa bongata le boholo pakeng tsa dikarolo. Qalong, mohato wa ho arola dipharologanyo tsa ...

III. Ho Fokotsa Bokgoni ba Methapo e Meholo: Moeli o Monyenyane Pakeng tsa Bokgoni le ho Sebetsana

Ho centrifugation ea gradient ea density ke mokhoa o mong o sebelisoang bakeng sa karohano ea protheine le mafura. Mokhoeng ona, lisampole li beoa holim'a sebaka se nang le gradient ea density, joalo ka sucrose kapa cesium chloride, ka har'a tube ea centrifuge. Ha sampole e ntse e feta centrifugeng, lintho li tsamaea ka har'a gradient, li arohana ho latela bongata ba tsona bo phaphametseng. Mokhoa ona o nolofalletsa ho aroloa ha meaho e bonolo, joalo ka lipoprotein, tse nang le liprotheine le mafura.

IV. Liphephetso tsa Karohano ea Protheine le Lipid: Ho Hlōla Boemo bo Khomarelang

Bothata bo bong ba tlhaho ba ho arola liprotheine ho lipids bo ho kamano ea tsona. Liprotheine le lipids hangata lia sebelisana 'me li theha metsoako, e leng se etsang hore ho be thata ho li arola ka ho li centrifugation feela. Ho rarolla phephetso ena, sesepa se bonolo kapa mekhoa ea ho rarolla mathata a tlhaho e ka sebelisoa ho sitisa likamano tsa protheine le lipid. Leha ho le joalo, ho fumana maemo a matle a bolokang botšepehi ba sampole ha ho ntse ho nolofalletsa karohano e ntse e le ts'ebetso ea teko le phoso.

V. Mekhoa e Tsoetseng Pele ea ho Fokotsa Matla: Ka Nģ'ane ho Mekhoa e Tloaelehileng

Bafuputsi ba ntse ba tsoela pele ho nts'etsapele mekhoa e tsoetseng pele ea ho kopanya liprotheine le mafura ho ntlafatsa katleho ea karohano ea protheine le mafura. Mokhoa o mong o joalo ke ho kopanya liprotheine le liprotheine ka libaka kapa ka isopycnic, e sebelisang li-gradients tsa density tse nang le mekhahlelo e mengata ho arola limolek'hule tsa thickness tse fapaneng. Mokhoa ona o lumella karohano e nepahetseng empa o hloka lisebelisoa le boiphihlelo bo khethehileng ho sebetsa ka katleho. Ho phaella moo, mekhoa e mecha e kang ho kopanya li-centrifugation ka matla le ho kopanya li-centrifugation ka lebelo le phahameng e ka fana ka mekhoa e mecha ea ho sebetsana le maemo a rarahaneng le ho feta a karohano.

VI. Litšebeliso: Ho Senola Mekhoa ea Lisele le Litsela tsa Mafu

Karohano ea protheine le mafura ka ho sebelisa centrifugation e na le litlamorao tse kholo libakeng tse fapaneng tsa lipatlisiso. Mohlala, ho utloisisa hore na liprotheine li sebelisana joang le mafura ka har'a lera la sele ho senola temohisiso ea bohlokoa mabapi le phetisetso ea matšoao a sele le litsela tsa lefu. Ka ho arola liprotheine kapa mafura a itseng, bo-rasaense ba ka senola mesebetsi ea bona lits'ebetsong tsa 'mele tse kang ho mena ha protheine, ho falla ha sele le karohano ea sele.

Qetello: Mokhoa o Matla oa Lipatlisiso tsa Protheine le Lipid

Ho centrifugation ke sesebelisoa se sebetsang ka ho fetisisa bakeng sa karohano ea liprotheine ho lipids, leha ho hlahisa liphephetso ka lebaka la bongata ba tsona le likamano tsa tsona tse tšoanang. Ka ho sebelisa mekhoa e fapaneng ea ho centrifugation joalo ka ho centrifugation e fapaneng le ho centrifugation ea density gradient, bafuputsi ba ka arola le ho arola liprotheine le lipids ka katleho ho tsoa lisampoleng tse rarahaneng. Tsoelo-pele e tsoelang pele theknolojing ea ho centrifugation e bula tsela bakeng sa mekhoa e nepahetseng le e matla ea ho arola liprotheine le lipids, e nolofalletsang ho sibolloa ha mekhoa ea lisele le litsela tsa mafu.