Yini ongayihlukanisa nge-centrifuge

Isingeniso ku-Centrifugation kanye nezicelo zayo

Ukuhlukanisa Izingxenye Zegazi Zokusetshenziswa Kwezokwelapha

Ukuhlanza i-DNA ne-RNA kusetshenziswa i-Centrifuge

Ukuhlukanisa ukhilimu nobisi: Indlela Yokusebenzisa Ejwayelekile

Ukusetshenziswa Kwe-Centrifugation Ezimbonini: Ukuhlukanisa Uwoyela Namanzi

Isingeniso

I-Centrifugation iyindlela enamandla esetshenziswa ezimbonini ezahlukene kanye nasezifundweni zesayensi ukuhlukanisa nokuhlanza izingxenye ezahlukene zengxube. Ngokusebenzisa amandla e-centrifugal, i-centrifuge ingahlukanisa izinto ngokusekelwe ekubuneni kwazo nobukhulu bazo. Lesi sihloko sizohlola ububanzi bezicelo ze-centrifuge, okuhlanganisa ukuhlukanisa izingxenye zegazi, ukuhlanza i-DNA ne-RNA, ukuhlukanisa ukhilimu nobisi, kanye nokusetshenziswa kwezimboni njengokuhlukanisa uwoyela namanzi.

Ukuhlukanisa Izingxenye Zegazi Zokusetshenziswa Kwezokwelapha

Enye yezindlela ezaziwa kakhulu zokusebenzisa i-centrifugation isemkhakheni wezokwelapha, ikakhulukazi emabhange egazi nasema-laboratory. Ngokujikelezisa amasampula egazi ku-centrifuge, kungenzeka ukuhlukanisa izingxenye ezahlukene zokusetshenziswa okuhlukile kwezokwelapha.

Uma igazi lifakwa ku-centrifuge, lihlukana libe yizingqimba ezintathu ezihlukene: i-plasma, i-buffy coat, kanye namaseli egazi abomvu. I-plasma, ingxenye ewuketshezi yegazi, iqukethe amaprotheni, ama-electrolyte, ama-hormone, nezinye izinto ezisetshenziselwa ukuhlolwa kokuxilonga. I-buffy coat, ungqimba oluncane ngaphezu kwamaseli abomvu egazi, iqukethe amaseli egazi amhlophe nama-platelet, abalulekile ekumpompelweni nasekutholeni izifo. Okokugcina, amaseli egazi abomvu, akha iningi levolumu yegazi, avame ukusetshenziselwa ukumpontshelwa igazi ezigulini ezine-anemia noma ukulahlekelwa igazi.

Ukuhlanza i-DNA ne-RNA kusetshenziswa i-Centrifuge

I-Centrifugation idlala indima ebalulekile ku-biology yama-molecular kanye nocwaningo lwe-genetics, lapho ukuhlukaniswa nokuhlanzwa kwe-DNA ne-RNA kuyizinyathelo ezibalulekile. Ngokuhlukanisa lawa ma-nucleic acid kwezinye izingxenye zamaseli, ososayensi bangahlaziya futhi bafunde isakhiwo sawo, umsebenzi wawo, kanye nolwazi lwe-genetic.

Ukuze kuhlanzwe i-DNA noma i-RNA, isinyathelo sokuqala ukuvula amaseli bese kukhululwa ama-nucleic acid. Ngemuva kwalokho, ingxube ifakwa ku-centrifugation, okuvumela i-DNA noma i-RNA ukuthi iqhume phansi kwepayipi kuyilapho ukungcola okusele kweseli kuhlala ku-supernatant. Le nqubo iphindaphindwa izikhathi eziningana ukuqinisekisa ukuhlukaniswa okuphelele kanye nobumsulwa bama-nucleic acid.

Ukuhlukanisa ukhilimu nobisi: Indlela Yokusebenzisa Ejwayelekile

I-Centrifugation ibilokhu isetshenziswa embonini yobisi ukuhlukanisa ukhilimu nobisi nokukhiqiza imikhiqizo yobisi eyahlukahlukene. Ama-molecule amafutha obisini alula futhi awaqinile kangako kunezinye izingxenye; ngakho-ke, avame ukukhuphukela phezulu uma efakwa ku-centrifuge. Lokhu kuhlukaniswa kufezwa ngokuphotha ubisi ngokushesha ku-centrifuge, okubangela ukuthi ukhilimu uqoqeke phezulu.

Uma ukhilimu usuhlukanisiwe, ungacutshungulwa ube ibhotela, i-ghee, ukhilimu oqoshiwe, neminye imikhiqizo yobisi. Le nqubo ibalulekile ekufinyeleleni okuqukethwe kwamafutha okufiselekayo emikhiqizweni ehlukahlukene nokuqinisekisa ikhwalithi ehambisanayo.

Ukusetshenziswa Kwe-Centrifugation Ezimbonini: Ukuhlukanisa Uwoyela Namanzi

Ama-centrifuge asetshenziswa kakhulu ezimbonini ezifana nowoyela negesi, ukwelashwa kwamanzi amdaka, kanye nokukhiqizwa kwamakhemikhali ukuze kuhlukaniswe uketshezi olungaxubeki njengowoyela namanzi. Isimiso salokhu kuhlukaniswa ukuthi uketshezi olubili lunobukhulu obuhlukene, futhi ngokusebenzisa amandla e-centrifugal, lungahlukaniswa ngempumelelo.

Embonini kawoyela, isibonelo, ama-centrifuge amakhulu ezimboni asetshenziswa ukuhlukanisa uwoyela namanzi ngesikhathi senqubo yokucwenga uwoyela. Lokhu kuqinisekisa ukukhiqizwa kwemikhiqizo kawoyela esezingeni eliphezulu nehlanzekile. Ngokufanayo, ezitshalweni zokuhlanza amanzi amdaka, ama-centrifuge asetshenziswa ukuhlukanisa uwoyela nezinhlayiya eziqinile emanzini amdaka ngaphambi kokuba akhishwe noma asetshenziswe kabusha.

Isiphetho

I-Centrifugation iyindlela eguquguqukayo nebalulekile esetshenziswa ezimbonini eziningi kanye nasezindaweni zesayensi. Amandla ayo okuhlukanisa izinto ngokusekelwe kubukhulu kanye nobuningi ayenza ibe usizo kakhulu ekusetshenzisweni njengokuhlukanisa izingxenye zegazi ukuze zisetshenziswe kwezokwelapha, ukuhlanza i-DNA ne-RNA, ukuhlukanisa ukhilimu nobisi, kanye nokuhlukanisa uwoyela namanzi ezinqubweni zezimboni. I-Centrifuge iyaqhubeka nokuguqula imikhakha eyahlukahlukene, ivumela abacwaningi kanye nezimboni ukuthi bakhiphe, bahlanze, futhi bahlaziye izingxenye ezahlukene ukuze kuthuthukiswe umphakathi.