Yintoni onokuyahlula nge-centrifuge
Intshayelelo kwiCentrifugation kunye nezicelo zayo
Ukwahlula iinxalenye zegazi ukuze zisetyenziswe kwezonyango
Ukucoca iDNA kunye neRNA kusetyenziswa iCentrifuge
Ukwahlula iCream kubisi: Indlela eqhelekileyo yokusebenzisa
Ukusetyenziswa kweCentrifugation kwimizi-mveliso: Ukwahlula ioyile namanzi
Intshayelelo
I-Centrifugation yindlela enamandla esetyenziswa kumashishini ahlukeneyo nakwiinkalo zesayensi ukwahlula nokucoca izinto ezahlukeneyo zomxube. Ngokusebenzisa amandla e-centrifugal, i-centrifuge inokwahlula izinto ngokusekelwe kubuninzi bazo kunye nobukhulu bazo. Eli nqaku liza kuhlola uluhlu olubanzi lwezicelo ze-centrifuges, kubandakanya ukwahlula izinto zegazi, ukucoca i-DNA kunye ne-RNA, ukwahlula ukhilimu kubisi, kunye nokusetyenziswa kwemizi-mveliso njengokwahlula ioyile namanzi.
Ukwahlula iinxalenye zegazi ukuze zisetyenziswe kwezonyango
Enye yezona ndlela zidumileyo zokusebenzisa i-centrifugation zikwicandelo lezonyango, ingakumbi kwiibhanki zegazi nakwiilebhu. Ngokujikelezisa iisampuli zegazi kwi-centrifuge, kunokwenzeka ukwahlulahlula izinto ezahlukeneyo ukuze zisetyenziswe kwezonyango ezahlukeneyo.
Xa igazi lifakwa kwi-centrifuge, liyahlukana libe ziingqimba ezintathu ezahlukeneyo: i-plasma, i-buffy coat, kunye neeseli ezibomvu zegazi. I-plasma, inxalenye engamanzi yegazi, ineeproteni, ii-electrolytes, iihomoni, kunye nezinye izinto ezisetyenziselwa uvavanyo lokuxilonga. I-buffy coat, umaleko omncinci ngaphezu kweeseli ezibomvu zegazi, ineeseli ezimhlophe zegazi kunye neeplatelets, ezibalulekileyo ekutofelweni igazi nasekuchongeni usulelo. Okokugqibela, iiseli ezibomvu zegazi, ezenza uninzi lwegazi, zihlala zisetyenziselwa utofelo kwizigulane ezine-anemia okanye ukulahleka kwegazi.
Ukucoca iDNA kunye neRNA kusetyenziswa iCentrifuge
I-Centrifugation idlala indima ebalulekileyo kwi-molecular biology kunye nophando lwe-genetics, apho ukwahlulwa kunye nokucocwa kwe-DNA kunye ne-RNA ngamanyathelo abalulekileyo. Ngokwahlula ezi nucleic acids kwezinye izinto zeseli, izazinzulu zinokuhlalutya kwaye zifunde ulwakhiwo lwazo, umsebenzi wazo, kunye nolwazi lwe-genetic.
Ukucoca i-DNA okanye i-RNA, inyathelo lokuqala kukuvula iiseli nokukhulula ii-nucleic acids. Emva koko, umxube ufakwa kwi-centrifugation, okuvumela i-DNA okanye i-RNA ukuba iphume ezantsi kwetyhubhu ngelixa inkunkuma esele isele kwi-supernatant. Le nkqubo iphindaphindwa amaxesha amaninzi ukuqinisekisa ukwahlukana okupheleleyo kunye nobunyulu bee-nucleic acids.
Ukwahlula iCream kubisi: Indlela eqhelekileyo yokusebenzisa
I-Centrifugation ibisoloko isetyenziswa kwishishini lobisi ukwahlula ukhilimu kubisi nokuvelisa iimveliso ezahlukeneyo zobisi. Iimolekyuli zamafutha kubisi zikhaphukhaphu kwaye azixinene kangako kunezinye izinto; ngoko ke, zihlala zinyuka ziye phezulu xa zifakwe kwi-centrifuge. Olu hluko lufezekiswa ngokujikelezisa ubisi ngokukhawuleza kwi-centrifuge, nto leyo ebangela ukuba ukhilimu uqokelele phezulu.
Nje ukuba ikhrimu yahlulwe, ingacutshungulwa ibe yibhotolo, ighee, ikhrimu etyumziweyo, kunye nezinye iimveliso zobisi. Le nkqubo ibalulekile ekufezekiseni umxholo wamafutha ofunekayo kwiimveliso ezahlukeneyo nokuqinisekisa umgangatho ohambelanayo.
Ukusetyenziswa kweCentrifugation kwimizi-mveliso: Ukwahlula ioyile namanzi
IiCentrifuge zisetyenziswa kakhulu kumashishini afana neoyile negesi, unyango lwamanzi amdaka, kunye nokwenziwa kweekhemikhali ukwahlula ulwelo olungaxutywanga njengeoyile namanzi. Umgaqo ongasemva kolu hluko kukuba ulwelo olubini lunobunzima obahlukeneyo, kwaye ngokusebenzisa amandla ecentrifugal, lunokwahlulwa ngokufanelekileyo.
Umzekelo, kwishishini leoyile, ii-centrifuge ezinkulu zoshishino zisetyenziselwa ukwahlula ioyile emanzini ngexesha lenkqubo yokucocwa kweoyile. Oku kuqinisekisa ukuveliswa kweemveliso zeoyile ezisemgangathweni ophezulu nezicocekileyo. Ngokufanayo, kwizityalo zonyango lwamanzi amdaka, ii-centrifuge zisetyenziselwa ukwahlula ioyile kunye namasuntswana aqinileyo kumanzi amdaka ngaphambi kokuba akhutshwe okanye asetyenziswe kwakhona.
Isiphelo
I-Centrifuge yindlela eguquguqukayo nebalulekileyo esetyenziswa kumacandelo amaninzi eemveliso kunye nesayensi. Ukukwazi kwayo ukwahlula izinto ngokusekelwe kubukhulu kunye nobuninzi kuyenza ibe luncedo kakhulu kwizicelo ezifana nokwahlula iinxalenye zegazi ukuze zisetyenziswe kwezonyango, ukucoca i-DNA kunye ne-RNA, ukwahlula ukhilimu kubisi, kunye nokwahlula ioyile namanzi kwiinkqubo zoshishino. I-Centrifuge iyaqhubeka nokuguqula amacandelo ahlukeneyo, ivumela abaphandi kunye namashishini ukuba bakhuphe, bacoce, kwaye bahlalutye iinxalenye ezahlukeneyo ukuze kuphuculwe uluntu.
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