koj tuaj yeem cais dab tsi nrog lub centrifuge
Kev Taw Qhia Txog Centrifugation thiab Nws Cov Ntawv Thov
Kev Cais Cov Cheebtsam Ntshav Rau Kev Siv Kho Mob
Kev ntxuav DNA thiab RNA siv lub Centrifuge
Kev Cais Cov Tshuaj Pleev Xim Los Ntawm Mis Nyuj: Ib Daim Ntawv Thov Classic
Kev Siv Centrifugation Hauv Kev Lag Luam: Kev Sib Cais Roj Thiab Dej
Kev Taw Qhia
Kev siv centrifugation yog ib txoj kev siv zog heev uas siv rau ntau yam lag luam thiab kev tshawb fawb los cais thiab ntxuav cov khoom sib xyaw ua ke. Los ntawm kev siv lub zog centrifugal, lub centrifuge tuaj yeem cais cov tshuaj raws li lawv qhov ceev thiab qhov loj. Tsab xov xwm no yuav tshawb nrhiav ntau yam kev siv rau centrifuges, suav nrog kev cais cov khoom ntshav, kev ntxuav DNA thiab RNA, kev cais cov mis nyuj tawm ntawm mis nyuj, thiab kev siv hauv kev lag luam xws li kev cais roj thiab dej.
Kev Cais Cov Cheebtsam Ntshav Rau Kev Siv Kho Mob
Ib qho ntawm cov kev siv centrifugation uas paub zoo tshaj plaws yog nyob rau hauv kev kho mob, tshwj xeeb tshaj yog nyob rau hauv cov txhab ntshav thiab cov chaw soj nstuam. Los ntawm kev tig cov ntshav hauv lub centrifuge, nws muaj peev xwm cais cov khoom sib txawv rau kev siv kho mob sib txawv.
Thaum cov ntshav raug centrifuged, nws faib ua peb txheej sib txawv: plasma, buffy coat, thiab cov qe ntshav liab. Plasma, qhov kua ntawm cov ntshav, muaj cov protein, electrolytes, hormones, thiab lwm yam khoom siv rau kev kuaj mob. Lub buffy coat, ib txheej nyias nyias saum cov qe ntshav liab, muaj cov qe ntshav dawb thiab platelets, uas tseem ceeb rau kev hloov ntshav thiab txheeb xyuas cov kab mob. Thaum kawg, cov qe ntshav liab, uas yog feem ntau ntawm cov ntshav, feem ntau siv rau kev hloov ntshav rau cov neeg mob uas muaj ntshav tsis txaus lossis poob ntshav.
Kev ntxuav DNA thiab RNA siv lub Centrifuge
Kev centrifugation ua lub luag haujlwm tseem ceeb hauv kev tshawb fawb txog molecular biology thiab kev tshawb fawb txog noob caj noob ces, qhov twg kev cais thiab kev ntxuav DNA thiab RNA yog cov kauj ruam tseem ceeb. Los ntawm kev cais cov nucleic acids no ntawm lwm cov khoom hauv cellular, cov kws tshawb fawb tuaj yeem tshuaj xyuas thiab kawm lawv cov qauv, kev ua haujlwm, thiab cov ntaub ntawv txog noob caj noob ces.
Yuav kom DNA lossis RNA ntshiab, thawj kauj ruam yog rhuav cov cell thiab tso cov nucleic acids tawm. Tom qab ntawd, cov khoom sib tov raug centrifugation, cia DNA lossis RNA pellet rau hauv qab ntawm lub raj thaum cov seem cellular khib nyiab tseem nyob hauv supernatant. Cov txheej txheem no rov ua dua ntau zaus kom ntseeg tau tias muaj kev sib cais tag nrho thiab huv si ntawm nucleic acids.
Kev Cais Cov Tshuaj Pleev Xim Los Ntawm Mis Nyuj: Ib Daim Ntawv Thov Classic
Kev siv tshuab centrifugation tau siv ntev los lawm hauv kev lag luam mis nyuj los cais cov mis nyuj qab zib thiab tsim ntau yam khoom mis nyuj. Cov roj molecules hauv mis nyuj sib dua thiab tsis ntom dua li lwm cov khoom; yog li, lawv feem ntau nce mus rau saum toj thaum centrifuged. Qhov kev sib cais no yog ua tiav los ntawm kev tig mis nyuj sai sai hauv lub centrifuge, ua rau cov mis nyuj sib sau ua ke rau saum toj.
Thaum cov mis nyuj sib cais lawm, nws tuaj yeem ua tiav rau hauv butter, ghee, whipped cream, thiab lwm yam khoom noj mis nyuj. Cov txheej txheem no yog qhov tseem ceeb rau kev ua tiav cov rog uas xav tau hauv ntau yam khoom thiab xyuas kom meej tias zoo ib yam.
Kev Siv Centrifugation Hauv Kev Lag Luam: Kev Sib Cais Roj Thiab Dej
Cov tshuab centrifuges siv dav hauv kev lag luam xws li roj thiab roj av, kev kho dej khib nyiab, thiab kev tsim tshuaj lom neeg los cais cov kua dej uas tsis sib xyaw xws li roj thiab dej. Lub hauv paus ntsiab lus tom qab kev sib cais no yog tias ob qho kua dej muaj qhov sib txawv, thiab los ntawm kev siv lub zog centrifugal, lawv tuaj yeem sib cais tau zoo.
Piv txwv li, hauv kev lag luam roj, cov tshuab centrifuges loj hauv kev lag luam siv los cais cov roj tawm ntawm dej thaum lub sijhawm ua roj. Qhov no ua kom ntseeg tau tias muaj cov khoom siv roj zoo thiab huv. Ib yam li ntawd, hauv cov chaw kho dej khib nyiab, cov tshuab centrifuges siv los cais cov roj thiab cov khoom khov ntawm cov dej khib nyiab ua ntej nws raug tso tawm lossis rov siv dua.
Xaus lus
Kev siv centrifugation yog ib txoj kev siv tau ntau yam thiab tseem ceeb heev uas siv rau ntau yam lag luam thiab kev tshawb fawb. Nws lub peev xwm los cais cov tshuaj raws li qhov loj thiab qhov ceev ua rau nws muaj txiaj ntsig zoo rau kev siv xws li kev cais cov khoom ntshav rau kev siv kho mob, kev ntxuav DNA thiab RNA, kev cais cov mis nyuj tawm ntawm mis nyuj, thiab kev cais cov roj thiab dej hauv cov txheej txheem kev lag luam. Cov centrifuges txuas ntxiv hloov pauv ntau yam haujlwm, ua rau cov kws tshawb fawb thiab cov lag luam rho tawm, ntxuav, thiab tshuaj xyuas cov khoom sib txawv rau kev txhim kho ntawm lub zej zog.
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