indlela yokuhlukanisa i-serum egazini nge-centrifuge

Isingeniso:

I-Centrifugation iyindlela esetshenziswa kabanzi yelabhorethri ehlukanisa izingxenye ezahlukene zesampula ngokusekelwe ekubuneni kwazo. Emkhakheni wezokwelapha, idlala indima ebalulekile ekuhlukaniseni i-serum egazini ngezinjongo zokuxilonga nokucwaninga. Lesi sihloko sizohlinzeka ngomhlahlandlela wesinyathelo ngesinyathelo wokuthi ungahlukanisa kanjani i-serum egazini usebenzisa i-centrifuge, kanye nezinyathelo zokuphepha ezidingekayo ukuqinisekisa imiphumela enembile nethembekile.

1. Ukuqonda i-Centrifugation kanye nokubaluleka kwayo ekuhlukanisweni kwe-Serum

- Incazelo kanye nesimiso sokusebenza se-centrifuge

- Ukubaluleka kokuhlukaniswa kwe-serum ekusetshenzisweni kwezokwelapha

Ngaphambi kokuba singene enkambisweni yokuhlukanisa i-serum egazini, kubalulekile ukuqonda ngokucacile i-centrifugation kanye nokufaneleka kwayo kulo mongo. I-centrifuge iyithuluzi lelebhu elisebenzisa amandla e-centrifugal ukuhlukanisa izinhlayiya ngaphakathi kwesampula yoketshezi ngokusekelwe ekubuneni noma usayizi wazo.

Ukuhlukaniswa kwe-serum egazini kubalulekile ekuphenyweni okuhlukahlukene kwezokwelapha, njengokuhlolwa kokuxilonga, ukuhlolwa kwamakhemikhali, kanye nocwaningo lwezokwelapha. I-serum yingxenye yegazi ewuketshezi esala ngemva kokujiya, engenawo amaseli nezinto ezibangela ukujiya kwegazi. Iqukethe izinhlobo eziningi zamaprotheni, ama-enzyme, ama-hormone, nama-ion anganikeza ukuqonda okubalulekile ngesimo sempilo yomuntu.

2. Ukulungiselela Amasampula Nemishini

- Izinto ezidingekayo ukuze kuhlukaniswe i-serum

- Ukuphathwa kahle kanye nokulebula amasampula egazi

Ngaphambi kokuqala inqubo ye-centrifugation, qiniseka ukuthi unazo zonke izinto ezidingekayo. Lokhu kufaka phakathi amapayipi okuqoqa igazi, umshini we-centrifuge, izinto zokuphepha ezifanele (njengegilavu ​​​​namajazi elebhu), kanye namapayipi akwazi ukugcina i-serum ehlukanisiwe.

Ukuphathwa kahle kanye nokulebula amasampula egazi kubalulekile ukuze kugwenywe noma yikuphi ukudideka noma amaphutha okuhlonza. Isampula ngayinye kufanele iphawulwe ngokucacile ngesihlonzi esiyingqayizivele, njengegama lesiguli noma inombolo yokuhlonza, ukuqinisekisa imiphumela enembile nokuvimbela ukudideka ngesikhathi sokuhlaziywa okulandelayo.

3. Ukusetha Amapharamitha E-Centrifuge

- Ukukhetha izilungiselelo ezifanele ze-centrifuge

- Ukunquma amandla nesikhathi se-centrifugal esifiselekayo

Isinyathelo esilandelayo sihilela ukusetha amapharamitha e-centrifuge ukuze kufezwe ukuhlukaniswa okuhle kakhulu. Izilungiselelo ezifanele ze-centrifuge zincike kakhulu ezidingweni ezithile zokuhlolwa noma ukuhlaziywa okwenziwayo.

Ukuze kuhlukaniswe i-serum egazini, amandla e-centrifugal asukela ku-1,500 kuya ku-2,000 amandla adonsela phansi (avezwa njengamandla e-centrifugal ahlobene, noma i-RCF) avame ukwanele. Isikhathi soku-centrifugation singahluka kuye ngobuningi kanye nohlobo lwesampula yegazi.

4. Ukulayisha i-Centrifuge kanye nokuqala inqubo yokuhlukanisa

- Ukuphathwa kahle kanye nokufakwa kwamasampula egazi ku-centrifuge

- Amathiphu okugcina ibhalansi nokuzinza ngesikhathi sokusebenza kwe-centrifugation

Faka amasampula egazi ngokucophelela ku-centrifuge, uqinisekise ukusatshalaliswa okulinganayo kuyo yonke i-rotor ukuze kulondolozwe ibhalansi ngesikhathi sokusebenza. Kubalulekile ukulayisha inani elilinganayo lamashubhu noma ukusebenzisa izinto eziphikisanayo ukuze kulondolozwe ukuzinza nokuvimbela ukulimala kwe-centrifuge.

Uma wonke amasampula eselayishwe kahle, vala isembozo se-centrifuge ngokuphephile bese uqala inqubo yokuhlukanisa. I-centrifuge izojikeleza amasampula ngokushesha, isebenzise amandla amakhulu e-centrifugal abangela ukuthi amangqamuzana abomvu egazi aminyene ahlale phansi kwepayipi, kuyilapho i-serum elula iqoqana phezulu.

5. Ukuqoqa Nokugcina I-Serum Ehlukanisiwe

- Amasu okuqoqwa kwe-serum efanele

- Izindlela ezinhle kakhulu zokugcina i-serum ukuze kulondolozwe ubuqotho besampula

Ngemva kokufaka i-centrifuge, susa ngokucophelela amapayipi ku-centrifuge ngaphandle kokuphazamisa izendlalelo ezihlukanisiwe. Usebenzisa i-pipette noma uhlelo olukhethekile lokuqoqa i-serum, khipha ungqimba lwe-serum ku-tube ngayinye, uqaphela ukugwema ukungcola.

Ukuze kugcinwe ubuqotho besampula ye-serum, kubalulekile ukuyigcina kahle. I-serum kufanele idluliselwe kumashubhu okugcina ahlanzekile futhi angenamagciwane, okungcono enziwe ngezinto ezingasebenzi njengengilazi, ukuze kugwenywe noma yikuphi ukuphazamiseka kokuhlaziywa kwelebhu okulandelayo. Amashubhu anelebula efanele kufanele agcinwe ekushiseni okufanele futhi avikelwe ekukhanyeni ukuze kuncishiswe ukuwohloka.

Isiphetho:

I-Centrifugation iyindlela ebalulekile yokuhlukanisa i-serum egazini kwezokwelapha kanye nocwaningo. Ngokulandela umhlahlandlela wesinyathelo ngesinyathelo onikezwe kulesi sihloko, abacwaningi kanye nochwepheshe bezokwelapha bangathola amasampula e-serum asezingeni eliphezulu ngokwethembeka ukuze kuhlolwe ngokunembile ukuxilonga kanye nophenyo lwesayensi. Njengoba i-serum iqukethe ulwazi olubalulekile mayelana nempilo yomuntu, ukwazi ubuciko bokuhlukanisa i-serum usebenzisa i-centrifuge kubalulekile kunoma yiliphi ilabhorethri elisebenza emkhakheni wezokwelapha.