indlela yokwahlula i-serum egazini nge-centrifuge
Intshayelelo:
I-Centrifugation yindlela esetyenziswa kakhulu elebhu eyahlulahlula izinto ezahlukeneyo zesampuli ngokusekelwe kubuninzi bazo. Kwicandelo lezonyango, idlala indima ebalulekileyo ekuhlukaniseni i-serum egazini ngeenjongo zokuxilonga nophando. Eli nqaku liza kubonelela ngesikhokelo senyathelo ngenyathelo sendlela yokwahlula i-serum egazini usebenzisa i-centrifuge, kunye namanyathelo okhuseleko afunekayo ukuqinisekisa iziphumo ezichanekileyo nezithembekileyo.
1. Ukuqonda i-Centrifugation kunye nokubaluleka kwayo ekwahlulweni kwe-Serum
- Inkcazo kunye nomgaqo wokusebenza we-centrifuge
- Ukubaluleka kokwahlulwa kwe-serum kwizicelo zonyango
Ngaphambi kokuba singene kwinkqubo yokwahlula i-serum egazini, kubalulekile ukuba siqonde ngokucacileyo i-centrifugation kunye nokubaluleka kwayo kule meko. I-centrifuge sisixhobo selabhoratri esisebenzisa amandla e-centrifugal ukwahlula amasuntswana ngaphakathi kwisampuli yolwelo ngokusekelwe kubuninzi okanye ubungakanani bawo.
Ukwahlulwa kwe-serum egazini kubalulekile kwiiphando ezahlukeneyo zonyango, ezinje ngovavanyo lokuxilonga, uvavanyo lwe-biochemical, kunye nophando lwezonyango. I-serum yinxalenye yolwelo lwegazi ehlala emva kokujiya, engenazo iiseli kunye nezinto ezibangela ukujiya kwegazi. Iqulethe uluhlu olubanzi lweeproteni, ii-enzymes, iihomoni, kunye nee-ion ezinokubonelela ngengcaciso ebalulekileyo ngemeko yempilo yomntu.
2. Ukulungiselela iiSampuli kunye nezixhobo
- Izinto ezifunekayo ukuze kwahlulwe i-serum
- Ukuphathwa kakuhle kunye nokulebhelisha iisampuli zegazi
Ngaphambi kokuba uqale inkqubo yokucoca igazi, qiniseka ukuba unazo zonke izinto ezifunekayo. Ezi ziquka iityhubhu zokuqokelela igazi, umatshini wokucoca igazi, izixhobo zokhuseleko ezifanelekileyo (ezifana neeglavu kunye neengubo zelebhu), kunye neetyhubhu ezikwaziyo ukugcina i-serum eyahlukileyo.
Ukuphathwa kakuhle kunye nokubhalwa kweesampuli zegazi kubalulekile ukuze kuthintelwe naluphi na ukudideka okanye iimpazamo zokuchonga. Isampulu nganye kufuneka iphawulwe ngokucacileyo ngesazisi esahlukileyo, njengegama lesigulana okanye inombolo yokuchonga, ukuqinisekisa iziphumo ezichanekileyo kunye nokuthintela ukudideka ngexesha lohlalutyo olulandelayo.
3. Ukuseta iiParameters zeCentrifuge
- Ukukhetha useto olufanelekileyo lwe-centrifuge
- Ukuchonga amandla e-centrifugal afunekayo kunye nexesha
Inyathelo elilandelayo libandakanya ukuseta iiparameter ze-centrifuge ukuze kufunyanwe ukwahlulwahlulwa okufanelekileyo. Useto olufanelekileyo lwe-centrifuge luxhomekeke kakhulu kwiimfuno ezithile zovavanyo okanye uhlalutyo olwenziwayo.
Ukuze kwahlulwe i-serum egazini, amandla e-centrifugal aqala kwi-1,500 ukuya kwi-2,000 amaxesha amandla omxhuzulane (achazwa njenge-relative centrifugal force, okanye i-RCF) ahlala anele. Ixesha le-centrifugation linokwahluka ngokuxhomekeke kumthamo kunye nohlobo lwesampuli yegazi.
4. Ukulayisha iCentrifuge kunye nokuqalisa inkqubo yokwahlulahlula
- Ukuphathwa kakuhle kunye nokufakwa kweesampuli zegazi kwi-centrifuge
- Iingcebiso zokugcina ibhalansi kunye nozinzo ngexesha lokufaka i-centrifugation
Faka iisampuli zegazi ngononophelo kwi-centrifuge, uqinisekise ukuba usasazo lulingana kwi-rotor ukuze kugcinwe ibhalansi ngexesha lokusebenza. Kubalulekile ukulayisha inani elilinganayo leetyhubhu okanye ukusebenzisa izinto ezichaseneyo ukugcina uzinzo nokuthintela umonakalo kwi-centrifuge.
Nje ukuba zonke iisampuli zilayishwe ngokufanelekileyo, vala isiciko se-centrifuge ngokukhuselekileyo uze uqalise inkqubo yokwahlula. I-centrifuge iya kujikeleza ngokukhawuleza iisampuli, isebenzise amandla amakhulu e-centrifugal abangela ukuba iiseli ezibomvu zegazi ezixineneyo zihlale ezantsi kwetyhubhu, ngelixa i-serum elula iqokelelana phezulu.
5. Ukuqokelela nokugcina iSerum ehlukanisiweyo
- Iindlela zokuqokelela i-serum ngokufanelekileyo
- Iindlela ezilungileyo zokugcina i-serum ukuze kugcinwe ukuthembeka kwesampuli
Emva kokufaka i-centrifuge, susa ngononophelo iityhubhu kwi-centrifuge ngaphandle kokuphazamisa iileya ezahluliweyo. Usebenzisa i-pipette okanye inkqubo ekhethekileyo yokuqokelela i-serum, khupha ileya ye-serum kwityhubhu nganye, ulumke ukuthintela ungcoliseko.
Ukuze kugcinwe ukuthembeka kwesampuli yeserum, kubalulekile ukuyigcina ngokufanelekileyo. I-serum kufuneka idluliselwe kwiityhubhu zokugcina ezicocekileyo nezingenazintsholongwane, okungcono zenziwe ngezinto ezingasebenziyo ezifana neglasi, ukuze kuthintelwe naluphi na uphazamiseko kuhlalutyo lwelebhu olulandelayo. Iityhubhu ezineelebheli ezifanelekileyo mazigcinwe kubushushu obufanelekileyo kwaye zikhuselwe ekukhanyeni ukuze kuncitshiswe ukubola.
Isiphelo:
I-Centrifugation yindlela ebalulekileyo yokwahlula i-serum egazini kwizicelo zonyango nophando. Ngokulandela isikhokelo senyathelo ngenyathelo esinikwe kweli nqaku, abaphandi kunye neengcali zonyango banokufumana ngokuthembekileyo iisampulu ze-serum ezikumgangatho ophezulu ukuze kuhlolwe ngokuchanekileyo ukuxilongwa kunye nophando lwesayensi. Njengoko i-serum inolwazi oluxabisekileyo malunga nempilo yomntu, ukwazi ubuchule bokwahlula i-serum usebenzisa i-centrifuge kubalulekile kuyo nayiphi na ilabhoratri esebenza kwicandelo lezonyango.
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