nzira yekuparadzanisa serum kubva kuropa uchishandisa centrifuge

Nhanganyaya:

Kuparadzanisa ropa neropa inzira inoshandiswa zvakanyanya murabhoritari inoparadzanisa zvikamu zvakasiyana zvemuenzaniso zvichienderana nehuwandu hwazvo. Mukurapa, inoita basa rakakosha mukuparadzanisa ropa neropa kuti zviongororwe uye zviongororwe. Chinyorwa chino chichapa gwara rematanho-nhanho rekuti ungaparadzanisa sei ropa neropa uchishandisa centrifuge, pamwe chete nematanho ekudzivirira anodiwa kuti uve nechokwadi chekuti mhinduro dzakarurama uye dzakavimbika.

1. Kunzwisisa Centrifugation uye Kukosha Kwayo Mukuparadzaniswa kweSerum

- Tsanangudzo uye musimboti wekushanda kwecentrifuge

- Kukosha kwekuparadzaniswa kweserum mukushandiswa kwekurapa

Tisati tanyatsoongorora nzira yekuparadzanisa serum kubva muropa, zvakakosha kuti tinzwisise zvakajeka nezve centrifugation uye kukosha kwayo munyaya iyi. Centrifuge chishandiso cherabhoritari chinoshandisa simba re centrifugal kuparadzanisa zvidimbu zviri musampuli yemvura zvichienderana nehuwandu hwazvo kana saizi.

Kupatsanurwa kweropa kubva muropa kwakakosha pakuferefeta kwakasiyana-siyana kwezvekurapa, zvakaita sekuongororwa kwekuongorora, kuongororwa kwemakemikari, uye kuferefeta kwezvekurapa. Serum chikamu cheropa chinosara mushure mekugwamba, chisina masero nezvinhu zvinogwamba. Ine mapuroteni akasiyana-siyana, maenzyme, mahormone, uye maion anogona kupa ruzivo rwakakosha nezvehutano hwemunhu.

2. Kugadzirira Sampuli neMidziyo

- Zvinhu zvinodiwa pakupatsanurwa kweserum

- Kubata nemazvo uye kunyora mazita emasampuli eropa

Usati watanga nzira ye centrifugation, iva nechokwadi chekuti une zvese zvinodiwa. Izvi zvinosanganisira machubhu ekuunganidza ropa, muchina we centrifuge, zvishandiso zvekuchengetedza (zvakaita semagurovhosi nemajasi emurabhoritari), uye machubhu anokwanisa kuchengetedza serum yakaparadzaniswa.

Kubata nemazvo uye kunyora mazita emasampuli eropa kwakakosha kudzivirira kusanganiswa kana zvikanganiso zvekuzivikanwa. Muenzaniso wega wega unofanirwa kunyorwa zvakajeka nechiratidzo chakasiyana, senge zita remurwere kana nhamba yekuzivikanwa, kuitira kuti pave nemhedzisiro chaiyo uye kudzivirira kuvhiringidzika panguva yekuongorora kunotevera.

3. Kugadzirisa Maparamita eCentrifuge

- Kusarudzwa kwenzvimbo dzakakodzera dze centrifuge

- Kuziva simba repakati rinodiwa nenguva

Danho rinotevera rinosanganisira kugadzira ma parameter e centrifuge kuti pave nekupatsanurwa kwakakwana. Magadzirirwo akakodzera e centrifuge anonyanya kuenderana nezvinodiwa chaizvo zvebvunzo kana ongororo iri kuitwa.

Kuti ropa riparadzaniswe neropa, simba repakati pesimba rinotangira pa1,500 kusvika 2,000 kupfuura simba regiravhiti (rinoratidzwa sesimba repakati pesimba, kana kuti RCF) rinowanzo kukwana. Nguva yepakati pesimba inogona kusiyana zvichienderana nehuwandu uye rudzi rweropa ratorwa.

4. Kuisa Centrifuge muCentrifuge uye Kutanga Maitiro Ekuparadzanisa

- Kubata nemazvo uye kurodha masampuli eropa mu centrifuge

- Mazano ekuchengetedza kuenzana uye kugadzikana panguva ye centrifugation

Isa ropa nemazvo mu centrifuge, uchiita kuti rotor ipararire zvakaenzana panguva yekushanda. Zvakakosha kuisa machubhu akawanda zvakaenzana kana kushandisa zvinhu zvinotsiva ropa kuti rirambe rakagadzikana uye kudzivirira kukuvara kwe centrifuge.

Kana sampuli dzese dzaiswa zvakanaka, vhara chivharo che centrifuge zvakanaka wotanga nzira yekuparadzanisa. Centrifuge ichatenderedza sampuli nekukurumidza, ichishandisa simba guru re centrifugal rinoita kuti masero matsvuku eropa agare pasi pechubhu, ukuwo serum yakareruka ichiungana pamusoro.

5. Kuunganidza neKuchengeta Serum Yakaparadzaniswa

- Maitiro ekuunganidza serum nemazvo

- Nzira dzakanakisisa dzekuchengetedza serum kuti irambe yakasimba

Mushure mekupinza mvura mu centrifuge, bvisai machubhu acho nokungwarira musingakanganisi machubhu akaparadzaniswa. Uchishandisa pipette kana system yekuunganidza serum, bvisai serum layer kubva muchubhu yega yega, muchingwarira kudzivirira kusvibiswa.

Kuti sampuli yeropa irambe yakasimba, zvakakosha kuichengeta nemazvo. Serum inofanira kutamisirwa kumachubhu ekuchengetera akachena uye asina utachiona, zviri nani akagadzirwa nezvinhu zvisina simba zvakaita segirazi, kudzivirira chero kukanganiswa nekuongororwa kwerabhoritari kunotevera. Machubhu ane mazita akakodzera anofanira kuchengetwa patembiricha yakakodzera uye kudzivirirwa kubva kuchiedza kuderedza kuora.

Mhedziso:

Kubvisa ropa kubva muropa (centrifuge) inzira inokosha yekuparadzanisa ropa kubva muropa mukurapa nekutsvaga. Nekutevera gwara rinotaurwa muchinyorwa chino, vaongorori nenyanzvi dzezvekurapa vanogona kuwana samples dzeropa dzemhando yepamusoro kuti vaongororwe nemazvo uye kuti vaongorore zvesainzi. Sezvo ropa riine ruzivo rwakakosha maererano nehutano hwemunhu, kugona kupatsanura ropa uchishandisa centrifuge kwakakosha kune chero rabhoritari inoshanda mumunda wekurapa.